Ki-67 Prognostic Value in Different Histological Grades of Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma

Abstract

Introduction:Abnormal cell proliferation appears to be a possible predictor of tumorigenesis, Ki-67 protein expression is closely related to the cell proliferation and could be used as a biomarker for the growth in the most of human tumors. The aim of the study: Investigating of Ki-67 expression in the pathological grades of oral epithelial dysplasia and oral squamous cell carcinomas.Materials and Methods:The sample consisted of 30 formalin-fixed, paraffin-embedded specimens of oral epithelial dysplasia (OED), 30 other of oral squamous cell carcinomas (OSCC), and 10 normal oral epithelium (NOE) were conventionally stained with hematoxylin and eosin and immunohistochemically stained with Ki-67 monoclonal antibody.Results:Expression of Ki-67 was restricted to the basal layers in the normal oral epithelium whereas Ki-67 positive cells in oral epithelial dysplasia (OED) were located in the basal, suprabasal and spinous layers, Ki-67 expression was increased in high-risk cases. Ki-67 positive cells in well-differentiated (OSCC) were located mainly in the periphery of the tumor nests, in moderately-differentiated (OSCC) were located in both peripheral and part of a center of the tumor nests whereas it was diffused in most of the Poorly-differentiated (OSCC). Statistical analysis indicated a significant difference between the expression in (OED) and (NOE), (OSCC) and (NOE), and no differences between (OED) and (OSCC).Conclusion:This study has concluded that Ki-67 antigen could be used as a marker for the histological grading of OED and OSCC, Expression of Ki 67 increased according to the severity of oral epithelial dysplasia.

p63+Krt5+ basal cells are increased in the squamous metaplastic epithelium of patients with radiation-induced chronic Rhinosinusitis

“…Ki-67 is a nuclear protein present during all active phases of the cell cycle (G1, S, G2, and M), but is absent in resting cells (G0) [31]. Ki67 + cells are mainly located along the basal layer of nasal epithelium and specifically located in basal cells, indicating that cell proliferation in nasal epithelium mainly occurred in basal cells [16].…”

An immunohistochemical study showing Ki-67 as an analytical marker in oral malignant and premalignant lesions

“…No statistical noteworthy distinction was present amid NOE and mild OED, this shows that the pace of carcinomatous change is more in entities that display moderate to severe dysplasia. [ 11 ] There was a statistical noteworthy distinction in the expression of Ki-67 among well-differentiated OSCC, moderately differentiated OSCC, poorly differentiated OSCC, and NOM, this shows that the appearance of Ki‑67 amplified progressively with the increase of differentiation of OSCC. Macluskey et al [ 12 ] showed statistical noteworthy relation with mean Ki‑67 in healthy tissue, dysplasia, and in carcinomatous tissue, thus suggestive of increase in epithelial propagation throughout the changeover from dysplasia to cancer [ Table 3 ].…”

“…We saw that the cell proliferation in OSCC expanded with increasing histological grades of OSCC. Comparable outcomes were accounted by Takkem et al [ 11 ] and Tumuluri et al [ 20 ]…”

p63+Krt5+ Basal Cells are Increased in the Squamous Metaplastic Epithelium of Patients with Radiation-induced Chronic Rhinosinusitis

“…Ki-67 is a nuclear protein present during all active phases of the cell cycle (G1, S, G2, and M), but is absent in resting cells (G0) [29]. Ki67 + cells are mainly located along the basal layer of nasal epithelium and speci cally located in basal cells, indicating that cell proliferation in nasal epithelium mainly occurred in basal cells [16].…”