Kicked by Mos and tuned by MPF—the initiation of the MAPK cascade in<i>Xenopus</i>oocytes

Russo, Christophe; Beaujois, Rémy; Bodart, Jean‐François; Blossey, Ralf

doi:10.2976/1.3265771

Cited by 6 publications

(7 citation statements)

References 64 publications

(80 reference statements)

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“…These observations indicate that the microinjection of the PIP region of PfRCC-PIP and the presence of progesterone are clearly two synergized events to induce GVBD. Of note, the progesterone-induced GVBD in Xenopus oocytes is characterized by feedback loops of the downstream MAPK and MPF (cdc2/cyclinB) signaling cascades that are capable to potentiate upstream events ( Ferrell and Machleder, 1998 ; Russo et al, 2009 ). GVBD induced by low concentrations of progesterone can be potentiated by extracellular addition of insulin growth factor at a concentration that had no apparent effect on GVBD on its own or by intracellular injections of modulators that directly target kinases and/or phosphatases involved in these feedback events.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Protein Phosphatase Type-1 and a Kinase Anchoring Protein in Plasmodium falciparum

et al. 2018

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With its multiple regulatory partners, the conserved Protein Phosphatase type-1 (PP1) plays a central role in many functions of the biology of eukaryotic cells, including Plasmodium falciparum. Here, we characterized a protein named PfRCC-PIP, as a major partner of PfPP1. We established its direct interaction in vitro and its presence in complex with PfPP1 in the parasite. The use of Xenopus oocyte model revealed that RCC-PIP can interact with the endogenous PP1 and act in synergy with suboptimal doses of progesterone to trigger oocyte maturation, suggesting a regulatory effect on PP1. Reverse genetic studies suggested an essential role for RCC-PIP since no viable knock-out parasites could be obtained. Further, we demonstrated the capacity of protein region containing RCC1 motifs to interact with the parasite kinase CDPK7. These data suggest that this protein is both a kinase and a phosphatase anchoring protein that could provide a platform to regulate phosphorylation/dephosphorylation processes.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Protein Phosphatase Type-1 and a Kinase Anchoring Protein in Plasmodium falciparum

et al. 2018

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“…During meiotic maturation, its main activator is Mos and its main target is Rsk [26] . Activation of Mos above a threshold level triggers a phosphorylation cascade of MAP kinases characterized by a sharp all-or-none activation which is amplified by the feedback phosphorylation of Mos by ERK [27] – [29] . The activity of MAP kinases and of their main upstream and target components have been implicated in many control points of MPF activity.…”

Section: Resultsmentioning

confidence: 99%

A Dynamical Model of Oocyte Maturation Unveils Precisely Orchestrated Meiotic Decisions

et al. 2012

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Maturation of vertebrate oocytes into haploid gametes relies on two consecutive meioses without intervening DNA replication. The temporal sequence of cellular transitions driving eggs from G2 arrest to meiosis I (MI) and then to meiosis II (MII) is controlled by the interplay between cyclin-dependent and mitogen-activated protein kinases. In this paper, we propose a dynamical model of the molecular network that orchestrates maturation of Xenopus laevis oocytes. Our model reproduces the core features of maturation progression, including the characteristic non-monotonous time course of cyclin-Cdks, and unveils the network design principles underlying a precise sequence of meiotic decisions, as captured by bifurcation and sensitivity analyses. Firstly, a coherent and sharp meiotic resumption is triggered by the concerted action of positive feedback loops post-translationally activating cyclin-Cdks. Secondly, meiotic transition is driven by the dynamic antagonism between positive and negative feedback loops controlling cyclin turnover. Our findings reveal a highly modular network in which the coordination of distinct regulatory schemes ensures both reliable and flexible cell-cycle decisions.

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“…Consistent with this hypothesis, it was reported that at low levels of MAPK–phosphatase, a system stimulated by PDGF exhibits bistable states and that increasing the levels of MAPK–phosphatase drives the system into a monostable regime [Bhalla et al, 2002]. One has also to note that MPF play a crucial role in the dynamics of Erk activity, potentially enabling the tuning of the cascade [Russo et al, 2009a] while fine tuning of the threshold system itself could be achieved by a nested feedback loop, engaging the inhibition of the glycogen synthase kinase 3‐β (Gsk3‐β) [Justman et al, 2009].…”

Section: Case Study Of Female Gametes: All‐or‐none Erk Responsementioning

confidence: 88%

Extracellular‐regulated kinase—mitogen‐activated protein kinase cascade: Unsolved issues

Bodart

2010

J of Cellular Biochemistry

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This review point out several aspects regarding the mitogen-activated protein kinase (MAPK)/extracellular-regulated kinase (Erk) network, which are still pending issues in the understanding how this pathway integrate information to drive cell fates. Focusing on the role of Erk during cell cycle, it has to be underlined that Erk downstream effectors, which are required for mitosis progression and contribute to aneuploidy during tumorigenesis, remain to be determined. In addition to the identity of the terminal enzymes or effectors of Erk, it has to be stressed that the dynamic nature of the Erk signal is itself a key factor in cell phenotype decisions. Development of biophotonics strategies for monitoring the Erk network at the spatiotemporal level in living cells, as well as computational and hypothesis-driven approaches, are called to unravel the principles by which signaling networks create biochemical and biological specificities. Finally, Erk dynamics might also be impacted by other post-translational modification than phosphorylation, such as O-GlcNAcylation.

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Kicked by Mos and tuned by MPF—the initiation of the MAPK cascade inXenopusoocytes

Cited by 6 publications

References 64 publications

Characterization of a Protein Phosphatase Type-1 and a Kinase Anchoring Protein in Plasmodium falciparum

Characterization of a Protein Phosphatase Type-1 and a Kinase Anchoring Protein in Plasmodium falciparum

A Dynamical Model of Oocyte Maturation Unveils Precisely Orchestrated Meiotic Decisions

Extracellular‐regulated kinase—mitogen‐activated protein kinase cascade: Unsolved issues

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