Kinematic study on the effect of pH on bull sperm function

Contri, Alberto; Gloria, Alessia; Robbe, Domenico; Valorz, Claudio; Wegher, Laura; Carluccio, Augusto

doi:10.1016/j.anireprosci.2012.11.008

Cited by 45 publications

(49 citation statements)

References 41 publications

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“…Normalmente, as avaliações mais rotineiras realizadas para determinar a qualidade seminal englobam os testes de motilidade e morfologia espermática (MAZIERO et al, 2009), além de técnicas para avaliar a integridade e função da membrana plasmática do espermatozoide, como o teste de eosina-nigrosina e o teste hiposmótico (BARROS et al, 2007;SELVARAJU;GHOSH;RAVINDRA, 2009). De forma complementar, técnicas mais elaboradas foram desenvolvidas mais recentemente para aumentar o grau de precisão das avaliações andrológicas, como a análise computadorizada da motilidade espermática (CASA -Computer Assisted Sperm Analisys) (CONTRI et al, 2010;CONTRI et al, 2013;HUSSAIN;LESSARD;ANZAR, 2013) e as técnicas baseadas no uso de sondas fluorescentes (CELEGHINI et al, 2007;KOONJAENAK et al, 2007).…”

Section: Introductionunclassified

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

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This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility (P>0.05), but palm oil supplementation presented a positive effect on spermatic ability to progress (derived from BCF, STR and LIN) in Period IV (P<0.05). High correlation (91%) was observed between IP-H342 and E-N techniques (Y = 0.9155 x + 2.9843, R 2 = 0.82, P≤0.05). Thus, supplementation with UFAs increased the progressivity of spermatozoa after 90 days, and had no effect on other seminal parameters. Eosin-nigrosin stain can be used efficiently as a predictor of the level of PMI when conditions for implementing fluorescent probes not present.

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Section: Introductionunclassified

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

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“…It was found that total motility of spermatozoa had similar values at pH 6 to pH 7; however, the sperm motility was reduced at pH 5 and 8.5. (Contri et al., ). Rheotaxis, on the other hand, was sensitive to pH value.…”

Section: Discussionmentioning

confidence: 99%

Effect of pH on rheotaxis of bull sperm using microfluidics

El-sherry

Abdel-Ghani

Abou-Khalil

et al. 2017

Reprod Domestic Animals

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The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected from bulls was diluted with SOF medium (1:10). pH of the medium was adjusted using a digital pH meter to the following pH values: 6.0, 6.2, 6.4, 6.4, 6.8, 7.0. All kinetic parameters of sperm (n = 3,385) were determined through a computer-assisted sperm analysis (CASA) system using microfluidic devices with controlled flow velocity. The following parameters were determined: total motility (TM%), positive rheotaxis (PR%), straightline velocity (VSL, μm/s), average path velocity (VAP, μm/s), linearity (LIN, as VSL/VCL, %), beat cross-frequency (BCF, Hz) and curvilinear velocity (VCL, μm/s). Nitric oxide, calcium and potassium were estimated in semen at different pH values. To confirm the effect of nitric oxide and K , we used sodium nitroprusside (an NO donor) and KCL as (a K donor) to see their effect on sperm PR%. The results showed no difference in TM% at pH (6-7). The PR% was the lowest at pH 6 and 7. The best parameters for the PR% were at pH 6.4-6.6. The concentration of Ca did not change at different pH values. The mean NO values decreased with the increase of pH; however, the mean values of K increased with the increase of pH. Addition of high concentration of NO and K to the semen media at fixed pH level had a negative effect on TM% and PR%. In conclusion, the bull sperm had the best rheotaxis properties at pH 6.4-6.6 and sensitive to the change of seminal NO and K .

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“…An acid environment is required for sperm to mature as they traverse the epididymis. However, an alkaline luminal pH is required to initiate motility and to induce fertilizing capacity of sperm cells (8,30,36). The critical role of CFTR in the male reproductive system has been demonstrated by the magnitude of infertility that is associated with CFTR mutations including the high frequency of CBAVD in both severely and mildly affected cystic fibrosis patients.…”

Section: Discussionmentioning

confidence: 99%