Kinetic analysis of inhibition of α-glucosidase by leaf powder from <i>Morus australis</i> and its component iminosugars

Qiao, Ying; Nakayama, J; Ikeuchi, Takeaki; Ito, Masaaki; Kimura, Toshiyuki; Kojima, Kenji; Takita, Teisuke; Yasukawa, Kiyoshi

doi:10.1080/09168451.2020.1783991

Cited by 15 publications

(10 citation statements)

References 22 publications

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“…We previously quantified iminosugars in M. australis leaf (Qiao et al., 2020). In this study, to quantify iminosugars in M. australis fruit, each of standard 1‐DNJ, fagomine, and GAL‐DNJ were applied to LC‐MS/MS to make a calibration curve.…”

Section: Resultsmentioning

confidence: 99%

“…Liquid chromatography‐MS/MS (LC‐MS/MS) analysis was carried out by the method we reported previously (Qiao et al., 2020). Briefly, powdered M. australis fruits (500 mg) were dissolved in 50% ethanol (5.0 ml) followed by sonication for 5 min and centrifugation (10,000 × g , 4°C) for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…Measurement was carried out as described previously (Qiao et al, 2020(Qiao et al, , 2021. Briefly, 70 µl of 0.1 M phosphate buffer (pH 6.0), 10 µl of maltose, sucrose, or isomaltose solution, and 10 µl of M. australis fruit extract, C3G, C3R, or glucose solution were mixed and incubated at 37 • C for 10 min.…”

Section: Measurement Of Maltase Sucrase and Isomaltase Activitiesmentioning

confidence: 99%

“…Elution gradient were carried out 20-60% solvent B for 0-2.0 min, 60% solvent B for 2.0-5.5 min, 60-20% solvent B for 5.5-5.6 min, and 20% solvent B for 5.6-8.0 min at a flow rate of 0.2 ml/min. Other conditions corresponded to those described previously (Qiao et al, 2020). To estimate the concentrations of 1-DNJ, fagomine, and GAL-DNJ in powdered M. australis fruit extracts, each iminosugar was used to make a standard curve.…”

Section: Liquid Chromatography-ms/ms Analysismentioning

confidence: 99%

“…Consumption of the M. australis leaf tea suppressed sucroseinduced increase in blood glucose and insulin levels in human (Kume et al, 2019(Kume et al, , 2021. We previously examined the inhibitory effects of powdered M. australis leaf extract and its component iminosugars 1-DNJ, fagomine, and 2-O-α-D-galactopyranosyl deoxynojirimycin (GAL-DNJ) (Qiao et al, 2020(Qiao et al, , 2021, showing that 1-DNJ is a major inhibitor of α-glucosidase in the M. australis leaf extract. In addition to iminosugars, M. australis contains a range of phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Qiao

Ikeda

Ito

et al. 2022

Journal of Food Science

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The inhibition of α‐amylase and α‐glucosidase are important for the maintenance of blood glucose level. Mammalian α‐glucosidase includes maltase–glucoamylase and sucrase–isomaltase complexes. In this study, we examined the inhibitory effects of Morus australis fruit extract and its components, that is, three iminosugars (1‐deoxynojirimycin [1‐DNJ], fagomine, and 2‐O‐α‐D‐galactopyranosyl deoxynojirimycin), two anthocyanins (cyanidin‐3‐glucoside and cyanidin‐3‐rutinoside), and glucose, against α‐amylase and α‐glucosidase. We also quantified the concentration of each component in M. australis fruit extract. The IC50 values of the fruit extracts of four M. australis subspecies were >10 mg/ml for α‐amylase, 1.1–1.7 mg/ml for maltase, 6.9–8.6 mg/ml for glucoamylase, 0.13–1.0 mg/ml for sucrase, and 0.46–1.4 mg/ml for isomaltase. When the IC50 value of each component and the concentration of each component in the fruit extract were taken into consideration, our results indicated that glucose are involved in the inhibition of α‐amylase, and 1‐DNJ and glucose are involved in the inhibition of α‐glucosidase. This is in contrast to that in M. australis leaf, neither anthocyanin nor glucose are contained, and 1‐DNJ is a main inhibitor. Practical Application It is widely accepted that inhibition of α‐amylase and α‐glucosidase is one of the strategies to treat type‐2 diabetes. Today, acarbose, miglitol, and voglibose are clinically used for this purpose. Our results that 1‐DNJ and anthocyanin are present in Morus australis fruit and are involved in the inhibition of α‐amylase and α‐glucosidase suggest that M. australis fruit is a healthy sweetener.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Measurement Of Maltase Sucrase and Isomaltase Activitiesmentioning

confidence: 99%

Section: Liquid Chromatography-ms/ms Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Qiao

Ikeda

Ito

et al. 2022

Journal of Food Science

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Fisetin, a major component derived from mulberry (Morus australis Poir.) leaves, prevents vascular abnormal contraction

2021

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Mulberry (Morus australis Poir.) leaves have long been consumed in the form of tea or tincture especially in Asia, owing to their high antioxidant and blood pressure-regulating properties. Although it is thought that vascular abnormal contraction may be involved in the blood pressure-suppressing effect, the effect of mulberry on vascular abnormal contraction is still unknown. Therefore, we investigated mulberry leaves as a potential source of bioactive compounds that prevent vascular abnormal contraction. Mulberry leaves were divided into fresh leaves and tea leaves and further classified according to the age of the tree: more or less than 20 years old, into roasted and unroasted. Mulberry fruits were also evaluated.We assessed the preventive effect of mulberry extracts on vascular abnormal contraction. Extracts from mulberry leaves of trees more than 20 years old showed a strong preventive effect on vascular abnormal contraction of human coronary artery smooth muscle cells. Therefore, to identify the active components in mulberry leaves, we fractionated the active fractions by gel filtration chromatography and reversed-phase high-performance liquid chromatography. The active fraction was further analyzed by mass spectrometry and nuclear magnetic resonance; an active component of the mulberry leaf extract was fisetin. In addition, our results indicated that the hydroxyl group at the C-3 position of fisetin is crucial for its activity. These results prove that fisetin is effective in preventing vascular abnormal contraction. Overall, mulberry leaves and fisetin are expected to be used in a wide range of fields such as functional foods, nutraceuticals, and drug targets.

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Identification of Potential α-Glucosidase Inhibitors from American Ginseng Processed Products by UHPLC-Q-Orbitrap/MS and Molecular Docking

Liang,

Liu,

Shao

et al. 2024

Food Biophysics

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Kinetic analysis of inhibition of α-glucosidase by leaf powder from Morus australis and its component iminosugars

Cited by 15 publications

References 22 publications

Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Inhibition of α‐amylase and α‐glucosidase by Morus australis fruit extract and its components iminosugar, anthocyanin, and glucose

Fisetin, a major component derived from mulberry (Morus australis Poir.) leaves, prevents vascular abnormal contraction

Identification of Potential α-Glucosidase Inhibitors from American Ginseng Processed Products by UHPLC-Q-Orbitrap/MS and Molecular Docking

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