Kinetic Modeling of Ethanol Batch Fermentation by Escherichia Coli FBWHR Using Hot-Water Sugar Maple Wood Extract Hydrolyzate as Substrate

Wang, Yang; Liu, Shijie

doi:10.3390/en7128411

Cited by 14 publications

(3 citation statements)

References 30 publications

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“…After hemicellulose and cellulose hydrolysis, a high concentration of hexoses and pentoses sugars can be obtained and subsequently fermented to produce different molecules such as organic acids or biofuels such as bioethanol. Bacteria such as Escherichia coli can metabolize hexoses and pentoses efficiently and to grow, in the presence or absence of oxygen, in minimum media without the addition of complex components . In addition, E. coli has been genetically modified to produce ethanol as the main product of fermentation and it has been demonstrated that ethanologenic E. coli strains can tolerate diverse toxic compounds present in lignocellulosic hydrolysates , including acetate …”

Section: Introductionmentioning

confidence: 99%

Volumetric oxygen transfer coefficient as a means of improving volumetric ethanol productivity and a criterion for scaling up ethanol production withEscherichia coli

Fernández‐Sandoval

Galíndez‐Mayer

Moss-Acosta

et al. 2016

J. Chem. Technol. Biotechnol.

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BACKGROUND: This study evaluated the influence of different micro-aerated conditions, including the aeration rate, the volumetric oxygen transfer coefficient (k L a) and the oxygen transfer rate (OTR), on improving ethanol productivity and scale-up of the fermentation step of the ethanologenic Escherichia coli strain MS04 in mineral medium supplemented with xylose, glucose, and sodium acetate. RESULTS: Growth and ethanol production results using 0.75 L of fermenter showed that micro-aeration (0.1 vvm, 400 rpm) improved the volumetric ethanol productivity and sugar consumption rate compared with the anaerobic condition (0 vvm, 400 rpm) or higher aeration rates (>0.2 vvm) without reducing significantly the ethanol yield. The k L a and the OTR were estimated and a k L a value of 7.2 h −1 was used as a criterion to scale-up the fermentation process from 0.75 L to 9.16 L and 110 L. During scale-up, the volumetric ethanol productivity and ethanol yield of consumed sugars were maintained at similar levels to those obtained in the laboratory in the 0.75 L fermenter. CONCLUSIONS: The controlled supply of low levels of oxygen promoted an increase in the concentration of biomass favoring the production and volumetric productivity of ethanol. The use of k L a allowed the fermentation step to be scaled up with ethanologenic E. coli maintaining similar ethanol yields and productivities.

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Section: Introductionmentioning

confidence: 99%

Volumetric oxygen transfer coefficient as a means of improving volumetric ethanol productivity and a criterion for scaling up ethanol production withEscherichia coli

Fernández‐Sandoval

Galíndez‐Mayer

Moss-Acosta

et al. 2016

J. Chem. Technol. Biotechnol.

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show abstract

“…Therefore, effect of these inhibitors on ethanol yield and productivity must be studied for commercialization of lignocellulosic ethanol process. (Nichols et al 2008;Pampulha and Loureiro-Das 2000;Abbott and Ingledew 2004;Graves et al 2000;Wang and Liu 2014).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of fermentation kinetics of acid-treated corn cob hydrolysate for xylose fermentation in the presence of acetic acid by Pichia stipitis

Kashid

Ghosalkar

2017

3 Biotech

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The efficient utilization of lignocellulosic biomass for ethanol production depends on the fermentability of the biomass hydrolysate obtained after pretreatment. In this work we evaluated the kinetics of ethanol production from xylose using Pichia stipitis in acid-treated corn cob hydrolysate. Acetic acid is one of the main inhibitors in corn cob hydrolysate that negatively impacts kinetics of xylose fermentation by P. stipitis. Unstructured kinetic model has been formulated that describes cell mass growth and ethanol production as a function of xylose, oxygen, ethanol, and acetic acid concentration. Kinetic parameters were estimated under different operating conditions affecting xylose fermentation. This is the first report on kinetics of xylose fermentation by P. stipitis which includes inhibition of acetic acid on growth and product formation. In the presence of acetic acid in the hydrolysate, the model accurately predicted reduction in maximum specific growth rate (from 0.23 to 0.15 h) and increase in ethanol yield per unit biomass (from 3 to 6.2 gg), which was also observed during experimental trials. Presence of acetic acid in the fermentation led to significant reduction in the cell growth rate, reduction in xylose consumption and ethanol production rate. The developed model accurately described physiological state of P. stipitis during corn cob hydrolysate fermentation. Proposed model can be used to predict the influence of xylose, ethanol, oxygen, and acetic acid concentration on cell growth and ethanol productivity in industrial fermentation.

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“…Nowadays alternate form of energy production, chemicals and fuels are being focused due to scarcity and high prices of available natural resources [1][2][3]. Biomass can be used as source of raw material to fulfill ongoing demand of fuel.…”

Section: Introductionmentioning

confidence: 99%

Production of Ethanol from Quetta Pinus halepensis by Fermentation

Ghouri¹,

Noor²,

Munawar³

2015

J Chem Eng Process Technol

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Pinus halepensis is wasted in large amount in Quetta, Pakistan. The direct discharge of Pinus halepensis enhances environmental pollution so it is urgent demand to treat this waste Pinus halepensis in such a way to produce beneficial products and reduce the environmental impacts. In this work Pinus halepensis are first crushed in jaw crusher to a size of 212 µm (65 mesh number) and 600 µm (28 mesh number) Tyler sieve standard. The crushed powder is then converted into brix by H 2 SO 4 (1N) hydrolysis at 363.15 K. The brix are then converted into ethanol by fermentation. During the experiments acid hydrolysis and various parameters such as pH of Pinus halepensis solutions, sugar brix, specific gravity of Pinus halepensis solutions and fermentation time were studied. The best conversion of Pinus halepensis to ethanol has been recorded at 4.5 pH. The statistical analysis was carried out in order to study the percentage contribution of different parameters and their effect on the production of ethanol.

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Kinetic Modeling of Ethanol Batch Fermentation by Escherichia Coli FBWHR Using Hot-Water Sugar Maple Wood Extract Hydrolyzate as Substrate

Cited by 14 publications

References 30 publications

Volumetric oxygen transfer coefficient as a means of improving volumetric ethanol productivity and a criterion for scaling up ethanol production withEscherichia coli

Volumetric oxygen transfer coefficient as a means of improving volumetric ethanol productivity and a criterion for scaling up ethanol production withEscherichia coli

Evaluation of fermentation kinetics of acid-treated corn cob hydrolysate for xylose fermentation in the presence of acetic acid by Pichia stipitis

Production of Ethanol from Quetta Pinus halepensis by Fermentation

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