Kinetic Parameters Determination of the Phenolic Derivatives Assimilation byTrichosporon CutaneumR57

Abstract

The kinetic parameters characteristic of the growth and degradation capacity of Trichosporon cutaneum R57 strain in relation to o-, m-, p-chlorophenol, o-, m-, p-cresol and o-, m-, p-nitrophenol are presented in this study. The investigated strain degraded up to 0.1 g/l of all mono-chlorophenols. A concentration of 0.2 g/l p-cresol was completely degraded whereas m-cresol was degraded to an extent of 32%. No degradation of o-cresol was observed. O-nitrophenol in concentration of 0.1 g/l was less readily degraded than the same concentration of m-nitrophenol and p-nitrophenol was not degraded. The Haldane equation has frequently been used to describe the biodegradation of toxic substrates but little is known about the kinetic constants for the biodegradation of aromatic compounds. The coefficients obtained confirmed the higher toxicity of the investigated phenol derivatives on Trichosporon cutaneum R57. The yield coefficient (Y) was least of all in the medium supplemented with o-chlorophenol where µ max was also minimum. The highest value of K i characteristic of p-cresol assimilation correlated with the ability of Trichosporon cutaneum R57 to degrade up to 0.4 g/l of this compound. The model prediction was compared with experimental data. It was found that the designed model described the trend of experimental data satisfactorily.

Phenol and cresol mixture degradation by the yeast Trichosporon cutaneum

“…The higher value of k s(cr) demonstrated the lower rate of cresol utilization than that established for phenol. Correspondingly k i(ph) had higher value than k i(cr) showing the stronger toxic effect of cresol on the T. cutaneum R57 strain development [24]. This conclusion was in accordance with interaction coefficients values received.…”

“…The degradation of 0.1 g/l m-cresol was almost completely accomplished (85%). The degradation of 0.1 or 0.2 g/l p-cresol was completed out in 24 h [24].…”

Bioremediation of 2-chlorophenol containing wastewater by aerobic granules-kinetics and toxicity

“…Zlateval et al [39] reported the degradation rate of 2-CP to be 350 mg2-CP gMLVSS −1 d −1 and kinetic parameters K s and K i to be 150 and 50 mg L −1 , respectively.…”

Biodegradation kinetics of o-cresol by Pseudomonas putida DSM 548 (pJP4) and o-cresol removal in a batch-recirculation bioreactor system

“…In the natural environment, the microorganisms perform a major role in the biodegradation of toxic chemicals. Phenol is the most widely focused compound in biodegradation studies (Essam et al 2010), even though a few of them consider cresol derivatives as a mixture of phenolic pollutants (Kar et al 1997;Zlateva et al 2005). Several microorganisms utilize cresol as a sole carbon source, despite of its toxicity (Maeda et al 2005).…”