Kinetic-Spectrophotometric Determination of Thiocyanate in Human Saliva Based on Landolt Effect in Presence of Astrafloxine FF

Abstract

In the present study a kinetic-spectrophotometric method for thiocyanate determination is described. The suggested method for the determination of thiocyanate is based on its "Landolt effect" on the reaction of bromate with hydrobromic acid, which leads to the formation of only one halogen bromine. The reaction was monitored spectrophotometrically at the maximum wavelength of astrafloxine FF light absorption at 535 nm. The absorbance of reactants mixture decreased with an increase of the reaction time. The calibration curve for thiocyanate determination was obtained in the concentration range of 0.03-2.0 μg mL −1 under the optimal conditions (pH 1.5; C BrO 3-= 7.6 × 10-4 mol L-1 ; C astrafloxine FF = 1 × 10-5 mol L-1). The limit of detection was 0.01 μg mL −1. The method was successfully applied to the determination of thiocyanate in human saliva samples with satisfactory results.

Highly sensitive kinetic spectrophotometric method for tramadol trace level detection and process optimization using response surface methodology

“…Kinetic spectrophotometric analytical methods become important means in trace analysis as various methods have been reported for the determination of trace amount of numerous elements. [15][16][17] The methods are based on the catalytic or inhibitory effect of the analyte on the reaction in colored (Vis) or colorless (UV) solutions. [18] The application of these methods offered some specific advantages such as improved selectivity and high sensitivity.…”

Comparative determination of sodium in saliva by atomic absorption spectrophotometry and flame photometry

“…Åêñïåðèìåíòàëüíà ÷àñòèíà Ðîç÷èí Na ç êîíöåíòðàö³ºþ 0,1 ìîëü/ë ãîòóâàëè ³ç ô³êñàíàëó, à ðîç÷èíè ç ìåíøèìè êîí-öåíòðàö³ÿìè -øëÿõîì â³äïîâ³äíîãî ðîçâåäåííÿ âèõ³äíîãî áåçïîñåðåäíüî ïåðåä âèêîðèñòàííÿì. Ìîäåëüí³ ðîç÷èíè ñëèíè ãîòóâàëè çã³äíî ç ïðîïîçèö³ºþ [10], à çàá³ð ñëèíè çä³éñíþâàëè â³äïîâ³äíî äî ðåêîìåíäàö³é [11,12]. Äëÿ ïðèãîòóâàííÿ ðîç÷èí³â âèêîðèñòîâóâàëè á³äèñòèëüîâàíó âîäó, à âñ³ âèêîðèñòîâóâàí³ ðåàêòèâè ìàëè êâàë³ô³êàö³þ íå íèae÷ó «õ.÷.».…”