Kinetic spectrophotometric methods for the determination of artificial sweetener (sucralose) in tablets

Abstract

Two simple and sensitive kinetic spectrophotometric methods for the determination of sucralose are described. The first method is based upon a kinetic investigation of the oxidation reaction of the drug with alkaline potassium permanganate at room temperature for a fixed time of 30 min. The absorbance of the green coloured manganate ions produced was measured at 610 nm. The second method is based on the reaction of sucralose with cerium (IV) ammonium sulfate in the presence of perchloric acid with the subsequent measurement of the excess unreacted cerium (IV) ammonium sulfate at 320 nm at a fixed time of 30 min in a thermostated water bath at 60 ± 1 °C. This principle is adopted to develop a kinetic method for sucralose determination. The absorbance concentration plots in both methods were rectilinear over the range 4-16 and 10-30 µg ml(-1) , for the first and second methods, respectively. The different experimental parameters affecting the development and stability of the colours were carefully studied and optimized. The determination of sucralose by rate constant method, fixed concentration method, and fixed-time method was also feasible with calibration equations obtained but the latter method was found to be more applicable. The two methods have been applied successfully to commercial tablets.

Toward Improving Medication Adherence: The Suppression of Bitter Taste in Edible Taste Films

“…Sucralose content of microspheres was assayed by the procedure of Youssef et al [ 49 ] except that a stock concentration of 5.5 mM KMnO 4 was used, and the incubation time was extended to 35 minutes. For the assay, sucralose and sucralose-containing microspheres were fully dissolved in HPLC-grade acetonitrile.…”

HPLC/Elsd Determination and Validation of Alpha Lipoic Acid and Sucralose in Bulk and in Their Pharmaceutical Dosage Forms

“…3 Sucralose (SUC) (Figure 1) is an official artificial and sugar substitute in USP. 14 Most ingested sucralose is not broken down by the body, so it is non-caloric. It is produced by the chlorination of sucrose.…”

“…3 Different HPLC with various detectors is reported as UV, 4,5 liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), 6 electrochemical (EC), 7,8 fluorimetry, 9,10 coulometric electrode array detector (CEAD), and an electrospray ionization mass spectrometer (ESI-MS), 11 DAD, 12 and capillary electrophoresis. 13 Literature review reveals several methodologies for SUC determination as Spectrophotometry, 14,15 TLC, 16 HPTLC, 17 DSC, 18 IR, 19 and FT-IR. 20 Many chromatographic methods for SUC determination with different detectors as UPLC coupled with DAD and CAD detectors 21 and HPLC coupled with (PAD-IC), 22 RID, 23 ELSD, 24 also Mass spectrometry applied as (SPE-LC-MS/MS), 25,26 triple quadrupole mass spectrometry 27 and liquid chromatography/time-of-flight mass spectrometry.…”