Kinetic studies on recombinant stem bromelain

Bala, Manju; Mel, Maizirwan; Jami, Mohammed Saedi; Amid, Azura; Salleh, Hamzah Mohd.

doi:10.4236/aer.2013.13006

Cited by 19 publications

(22 citation statements)

References 28 publications

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“…There are numerous therapeutic applications of the thiol protease in inflammation, arthritis, indigestion, hay fever, ulcers and pulmonary edema (Brien et al 2004;Beuth 2008;Fitzhugh et al 2008). Furthermore, it increases the absorption of antibiotics used for the treatment of bacterial infections (Bala et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…mercury, silver, copper, cobalt, zinc and trans-epoxysu-ccinyl-L-leucylamido {4-guanidino} butane, etc. (McGarvey and Christoffersen 1992;Shukor et al 2008;Masdor and Said 2011;Padma et al 2012;Bala et al 2013). In 2012, Marshall and Golden characterized bromelain from Morinda citrifolia (Noni) and observed the noncompetitive inhibition of bromalein by HgCl 2 .…”

Section: Introductionmentioning

confidence: 99%

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Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions

Kaur

Grewal

2014

J Food Sci Technol

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The kinetics of cysteine and divalent ion modulation viz. Ca 2+ , Cu 2+, Hg 2+ of fruit bromelain (EC 3.4.22.33) have been investigated in the present study. Kinetic studies revealed that at pH 4.5, cysteine induced V-type activation of bromelain catalyzed gelatin hydrolysis. At pH 3.5, Ca 2+ inhibited the enzyme noncompetitively, whereas, both K-and V-type activations of bromelain were observed in the presence of 0.5 mM Ca 2+ at pH 4.5 and 7.5. Bromelain was inhibited competitively at 0.6 mM Cu 2+ ions at pH 3.5, which changed to an uncompetitive inhibition at pH 4.5 and 7.5. An uncompetitive inhibition of bromelain catalyzed gelatin hydrolysis was observed in the presence of 0.6 mM Hg 2+ at pH 3.5 and 4.5. These findings suggest that divalent ions modulation of fruit bromelain is pH dependent.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions

Kaur

Grewal

2014

J Food Sci Technol

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“…Fruit bromelain presents an isoelectric point at 4.6 (Nadzirah et al, 2013) and molecular mass of 31 kDa (Souza et al, 2005). All commercially available bromelain is derived from the stem (Bala et al, 2013).…”

Section: Bromelainmentioning

confidence: 99%

“…In the food industry bromelain has been used for meat tenderization, brewing, baking (Ketnawa et al, 2012;Amid et al, 2011), prevention of browning of apple juice (Bernela et al, 2016), clarification of beer and as a food supplement (Bala et al, 2013). During the process of tenderization, bromelain promote softening of meat by breaking the fibrous material .…”

Section: Applications Of Bromelainmentioning

confidence: 99%

“…Bromelain has extensive applications as an active ingredient in tooth-whitening dentifrices and skin products to treat acne, wrinkles, and dry skin, reduce post-injection bruising and swelling (Arshad et al, 2014). It is used as an active ingredient to provide mild peeling effects (Bala et al, 2013) and as a cleansing agent (Chaurasiya and Hebbar, 2013).…”

Section: Applications Of Bromelainmentioning

confidence: 99%

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Bromelain Separation and Purification Processes From Pineapple Extract

Abreu

Figueiredo

2019

Braz. J. Chem. Eng.

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The concern about the effective use of agro-industrial residues from the food industry has increased. Bromelain is an enzyme mainly obtained from the pineapple plant (A. comosus), including its parts normally considered as waste. It is a high value-added product with a wide variety of applications such as in food, cosmetic and pharmaceutical industries. Many approaches have been used to purify this enzyme, but an efficient method to produce highly active and pure bromelain is still highly desirable. The investigated methods include ion exchange chromatography, precipitation, an aqueous two-phase system as well as affinity and filtration membranes. This paper gives an overview about the advantages and limitations of each one. Chronologic results and advances for these separation processes are also shown. The major tendency is the use of combined processes, such as hybrid or integrated ones.

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Semiconducting Polymer Nanoenzymes with Photothermic Activity for Enhanced Cancer Therapy

Xie

Huang

et al. 2018

Angewandte Chemie

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Regulation of enzyme activity is fundamentally challenging but practically meaningful for biology and medicine.However,noninvasive remote control of enzyme activity in living systems has been rarely demonstrated and exploited for therapy. Herein, we synthesizeasemiconducting polymer nanoenzyme with photothermic activity for enhanced cancer therapy. Upon near-infrared (NIR) light irradiation, the activity of the nanoenzyme can be enhanced by 3.5-fold to efficiently digest collagen in the tumor extracellular matrix (ECM), leading to enhanced nanoparticle accumulation in tumors and consequently improved photothermal therapy (PTT). This study thus provides ap romising strategy to remotely regulate enzyme activity for cancer therapy.

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Kinetic studies on recombinant stem bromelain

Abstract: ABSTRACT

Cited by 19 publications

References 28 publications

Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions

Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions

Bromelain Separation and Purification Processes From Pineapple Extract

Semiconducting Polymer Nanoenzymes with Photothermic Activity for Enhanced Cancer Therapy

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