Kinetic Study of Cell Proliferation of Saccharomyces cerevisiae Strains by Sedimentation/Steric Field Flow Fractionation in Situ

Abstract

The technique of Sedimentation/Steric Field Flow Fractionation (Sd/StFFF) is applied to the kinetic study of cells proliferation of Saccharomyces cerevisiae strains. The experimental parameter varied is the time from the preparation of the yeast sample dispersion in the culture medium. The determination of the size and mass distributions of the yeast cells is combined with the growth of the yeast cells and their life cycle. The experimental results are compared with those obtained by scanning electron microscopy (SEM) and those found in the literature. Useful conclusions concerning the budding and the fission of these yeast cells were extracted.

Analysis of relationship between cell cycle stage and apoptosis induction in K562 cells by sedimentation field-flow fractionation

“…A M71B4 Carpanelli engine (Bologna, Italy) associated with a Mininvert 370 pilot unit (Richard Systems, Les Ullis, France), controlled the rotating speed of the centrifuge baskets. Sedimentation fields were expressed in units of gravity, 1 g = 980 cm/s 2 , and calculated as previously described [37]. Cleaning and decontamination procedures have been previously described [26].…”

“…These advantages are based on the drastic limitation of cell-solid phase interaction by the use of: (1) a specific separation device: empty ribbon-like channel without stationary phase and (2) a device setup allowing the "Hyperlayer" elution mode, a size/density driven separation mechanism. Since the report of Caldwell et al [14] on mammalian cells, FFF, SdFFF and related technologies have been used in many biological fields such as hematology, microorganism analysis, biochemistry/biotechnology and molecular biology, neurology and cancer research [4,20,22,[31][32][33][34][35][36][37][38][39][40][41][42][43]7,[44][45][46][47][48]. Over several years, we have studied the use of SdFFF in cancer research to study chemical induced apoptosis or differentiation in cancer cell lines.…”

Sedimentation field-flow fractionation separation of proliferative and differentiated subpopulations during Ca2+-induced differentiation in HaCaT cells

“…In SdFFF, a multi-gravitational external field is generated by rotation of the separation channel in a rotor basket, constituting one of the most complex devices used in FFF separation [17,27,28]. Since the report of Caldwell et al [29] on mammalian cells, FFF, SdFFF and related technologies have been used in many biological fields such as hematology, microorganism analysis, biotechnology, molecular biology, neurology and cancer research [18,[30][31][32][33][34][35][36][37][38][39]. Over several years, we used SdFFF in cancer research to study chemical induced apoptosis or differentiation in cancer cell lines.…”