Kinetic study of polydopamine sphere synthesis using TRIS: relationship between synthesis conditions and final properties

García-Mayorga, Juan Carlos; Rosu, H. C.; Jasso-Salcedo, Alma Berenice; Escobar-Barrios, Vladimir Alonso

doi:10.1039/d2ra06669f

Cited by 18 publications

(8 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The thermal profile of pDA similarly presents four thermal events, and the overall behavior verifies the successful polymerization from DA. The first weight loss (25–100 °C) is attributed to the evaporation of weakly bound water due to high pDA hygroscopicity . The second weight loss (100–240 °C) proves the presence of intramolecular, strongly bonded water trapped after polymerization.…”

Section: Resultsmentioning

confidence: 99%

Biocompatible Hybrid Graphenic Thin Coatings on Flexible Substrates through Matrix-Assisted Pulsed Laser Evaporation (MAPLE)

Alfe,

Minopoli,

Tartaglia

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

This work reports the production of biocompatible thin layers for biomedical applications based on a graphene-like material (GL), a graphene-related material (GRM) obtained from carbon black. GL was combined in a hybrid fashion with polydopamine (pDA), a mussel-inspired water-resistant wet adhesive bonding obtained by the oxidative polymerization of dopamine (DA), and polyvinyl pyrrolidinone (PVP), a nontoxic synthetic polymer with intrinsic adhesion properties, to obtain a tighter adhesion of the thin layer to the substrate (silicone slices). Matrix-assisted pulsed laser evaporation (MAPLE) was used to coat PDMS slices with thin films of GL-pDA and GL-PVP directly from their frozen suspensions in water. The results indicate that the relevant chemical−physical characteristics of both thin films (evidenced by FTIR and AFM) were maintained after MAPLE deposition and that the films exhibit uniformity also at the nanometric level. After deposition, the GL-pDA and GL-PVP films underwent a biological survey toward murine fibroblasts (NIH3T3), human keratinocytes (HaCAT), and human cervical adenocarcinoma epithelial-like (HeLa) cells to assess the feasibility of this approach. Results indicate that both the GL-pDA and GL-PVP films did not perturb the biological parameters evaluated, including cytoskeleton alterations. Both hybrid films enhanced the effects of GL on cellular vitality across all cell lines. Specifically, the GL-pDA film exhibited a more stable effect over time (up to 72 h), whereas the GL-PVP film behaved similarly to the GL film in NIH3T3 and HeLa cell lines after long-term exposure. These promising results make the GL-pDA and GL-PVP films potential candidates for the manufacture of coated flexible devices for biomedical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Biocompatible Hybrid Graphenic Thin Coatings on Flexible Substrates through Matrix-Assisted Pulsed Laser Evaporation (MAPLE)

Alfe,

Minopoli,

Tartaglia

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…However, in our study, BC hydrogel showed an absorption peak at 260 nm, presumed to have come from the adherence of bacterial proteins or bacterial media components. The maximum absorbance of dopamine monomer lies at 280 nm [ 24 ]. On the other hand, BC/PDA-150 and BC/PDA-300 hydrogels containing polydopamine (PDA) change the biomaterial’s optical properties.…”

Section: Resultsmentioning

confidence: 99%

Polydopamine-Functionalized Bacterial Cellulose as Hydrogel Scaffolds for Skin Tissue Engineering

Narayanan

Bhaskar

Sudhakar

et al. 2023

Gels

View full text Add to dashboard Cite

Bacterial cellulose (BC) is a natural polysaccharide polymer hydrogel produced sustainably by the strain Gluconacetobacter hansenii under static conditions. Due to their biocompatibility, easy functionalization, and necessary physicochemical and mechanical properties, BC nanocomposites are attracting interest in therapeutic applications. In this study, we functionalized BC hydrogel with polydopamine (PDA) without toxic crosslinkers and used it in skin tissue engineering. The BC nanofibers in the hydrogel had a thickness of 77.8 ± 20.3 nm, and they could be used to produce hydrophilic, adhesive, and cytocompatible composite biomaterials for skin tissue engineering applications using PDA. Characterization techniques, namely Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and Raman spectroscopy, were performed to investigate the formation of polydopamine on the BC nanofibers. The XRD peaks for BC occur at 2θ = 14.65°, 16.69°, and 22.39°, which correspond to the planes of (100), (010), and (110) of cellulose type Iα. Raman spectroscopy confirmed the formation of PDA, as indicated by the presence of bands corresponding to the vibration of aromatic rings and aliphatic C–C and C–O stretching at 1336 and 1567 cm−1, respectively. FTIR confirmed the presence of peaks corresponding to PDA and BC in the BC/PDA hydrogel scaffolds at 3673, 3348, 2900, and 1052 cm−1, indicating the successful interaction of PDA with BC nanofibers, which was further corroborated by the SEM images. The tensile strength, swelling ratio, degradation, and surface wettability characteristics of the composite BC biomaterials were also investigated. The BC/PDA hydrogels with PDA-functionalized BC nanofibers demonstrated excellent tensile strength and water-wetting ability while maintaining the stability of the BC fibers. The enhanced cytocompatibility of the BC/PDA hydrogels was studied using the PrestoBlue assay. Culturing murine NIH/3T3 fibroblasts on BC/PDA hydrogels showed higher metabolic activity and enhanced proliferation. Additionally, it improved cell viability when using BC/PDA hydrogels. Thus, these BC/PDA composite biomaterials can be used as biocompatible natural alternatives to synthetic substitutes for skin tissue engineering and wound-dressing applications.

show abstract

“…PDA NPs were prepared through self-polymerization of dopamine in Tris-buffer (pH 8.5). 34 The obtained spherical NPs had an average hydrodynamic radius of 158.4 AE 39.2 nm and the characteristic negative surface potential (À29.2 AE 1.9 mV), owing to the abundant presence of imine and quinone moieties within the PDA backbone. 35,36 To obtain positively charged carriers and facilitate nucleic acid binding, PDA NPs were incubated with poly-L-arginine (pArg, M w = 5-15 kDa) under mild basic conditions (10 mM NH 4 OH, aq.)…”

Section: Nanoscale Horizonsmentioning

confidence: 99%

A catch-and-release nano-based gene delivery system

Franck,

Bistrovic Popov,

Ahmed

et al. 2023

Nanoscale Horiz.

View full text Add to dashboard Cite

show abstract

Kinetic study of polydopamine sphere synthesis using TRIS: relationship between synthesis conditions and final properties

Abstract: Synthesized PDA shows unexpected regular structure giving crystallinity. Kinetic parameters for spherical PDA are reported showing that polymerization depends upon TRIS concentration and temperature.

Cited by 18 publications

References 74 publications

Biocompatible Hybrid Graphenic Thin Coatings on Flexible Substrates through Matrix-Assisted Pulsed Laser Evaporation (MAPLE)

Biocompatible Hybrid Graphenic Thin Coatings on Flexible Substrates through Matrix-Assisted Pulsed Laser Evaporation (MAPLE)

Polydopamine-Functionalized Bacterial Cellulose as Hydrogel Scaffolds for Skin Tissue Engineering

A catch-and-release nano-based gene delivery system

Contact Info

Product

Resources

About