Kinetics of Agrobacterium tumefaciens ubiquinone-10 batch production

Gu, Song; Yang, Jianming; Yuan, Qipeng; Xue, Pei-Jian; Zheng, Zhiming; Zhang, Yu

doi:10.1016/j.procbio.2006.04.002

Cited by 34 publications

(18 citation statements)

References 20 publications

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“…ZUTEO3 after 38 h of the coupled fermentationextraction process was lower than that found in fed-batch process in previous reports [4][5][6][7][8], this study achieved the highest speciWc CoQ 10 content (Table 1). Agrobacterium tumefaciens exhibited the highest CoQ 10 concentration among all strains for CoQ 10 production.…”

Section: Evect Of Addition Time Of Cell Membrane Permeability Accelercontrasting

confidence: 62%

Enhanced production of CoQ10 by newly isolated Sphingomonas sp. ZUTEO3 with a coupled fermentation–extraction process

Zhong

Fang

Liu

et al. 2009

J Ind Microbiol Biotechnol

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The use of coenzyme Q(10) (CoQ(10)) as a complementary therapy in heart failure will increase in proportion to the growth of the ageing population and the expansion of statins consumption. Economical production of CoQ(10) by microbes will become more important due to the growing demands of the pharmaceutical industry. Process simplification and integration might be one desirable pathway for economic production of CoQ(10) by microbial fermentation. In this report, the effect of a coupled fermentation-extraction process on CoQ(10) production by newly isolated Sphingomonas sp. ZUTEO3 was evaluated. It was found that the CoQ(10) yield of the coupled process was significantly higher than that of the traditional process. As optimal conditions in our experiment, 2% soybean oil was added to the original culture to enhance cell membrane permeability, and 50 mL hexane was added to the 30 h culture as an extracting solvent for the subsequent coupled fermentation-extraction process. The maximal yield of CoQ(10) reached 43.2 mg/L and 32.5 mg/g dry cell weight after 38 h of total fermentation period. The coupled process represents one potential pathway for CoQ(10) production with even higher yield and lower cost. This is the first report of CoQ(10) production by Sphingomonas sp. using a coupled fermentation-extraction process.

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Section: Evect Of Addition Time Of Cell Membrane Permeability Accelercontrasting

confidence: 62%

Enhanced production of CoQ10 by newly isolated Sphingomonas sp. ZUTEO3 with a coupled fermentation–extraction process

Zhong

Fang

Liu

et al. 2009

J Ind Microbiol Biotechnol

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“…This value of the specific CoQ 10 content corresponded to 1.28 times more than that achieved from batch operation. However, the increase of CoQ 10 production in the fed-batch fermentation was not high, compared with the result of fed-batch culture by Ha et al [11] (1.3 times higher) or Gu et al [32] (3.5 times higher), which was carried out in laboratory-scale fermenters. This was caused probably by difficulty of scale-up and/or feeding strategy in fed-batch operation [21].…”

Section: Optimum Coq 10 Production In a Batch Fermentationcontrasting

confidence: 57%

“…To obtain more CoQ 10 accumulation in the fermenter, a fed-batch operation was adopted using the four-stage feeding strategy proposed by Gu et al [32]. The working volume in the fed-batch fermentation increased from 50 to 90 l by intermittent feeding of concentrated culture medium.…”

Section: Pilot-scale Fermentation For Coq 10 Productionmentioning

confidence: 99%

Coenzyme Q10 production in a 150-l reactor by a mutant strain of Rhodobacter sphaeroides

Kien

Kong

Lee

et al. 2010

J Ind Microbiol Biotechnol

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For the commercial production of CoQ(10), batch-type fermentations were attempted in a 150-l fermenter using a mutant strain of R. sphaeroides. Optimum temperature and initial aeration rate were found to be 30 degrees C and 2 vvm, respectively. Under optimum fermentation conditions, the maximum value of specific CoQ(10) content was achieved reproducibly as 6.34 mg/g DCW after 24 h, with 3.02 g/l of DCW. During the fermentation, aeration shift (from the adequate aeration at the early growth phase to the limited aeration in active cellular metabolism) was a key factor in CoQ(10) production for scale-up. A higher value of the specific CoQ(10) content (8.12 mg/g DCW) was achieved in fed-batch fermentation and comparable to those produced by the pilot-scale fed-batch fermentations of A. tumefaciens, which indicated that the mutant strain of R. sphaeroides used in this study was a potential high CoQ(10) producer. This is the first detailed study to demonstrate a pilot-scale production of CoQ(10) using a mutant strain of R. sphaeroides.

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“…A 10% ( v /v) seed culture was inoculated into a 10 L fermentor with a working volume of 7 L. The fermentation was done by altering the parameters like temperature (25°C, 30°C and 30°C), agitation (300, 500 and 600 rpm) and aeration (0.2 and 0.6 vvm) [20][21][22]. The continuous feeding was started after 24 h of growth with 20.5% cane molasses solution at the flow rate of 10 ml/h.…”

Section: Fermentation Optimizationmentioning

confidence: 99%

Process and Strain Development for Reduction of Broth Viscosity with Improved Yield in Coenzyme Q10 Fermentation by Agrobacterium tumefaciens ATCC 4452

Tokdar

Wani²,

Kumar³

et al. 2013

Ferment Technol

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Viscous nature of the fermentation broth has phenomenal influence on process conditions and parameters in a fermentor. Though broth rheology has attracted significant influence in process research, still there is a challenge to modify fluid dynamics of fermentation broth. During the production of coenzymeQ 10 (CoQ 10 ) by Agrobacterium tumefaciens ATCC 4452, the culture broth becomes highly viscous due to excessive synthesis of exopolysaccharides. This hinders the CoQ 10 yield and complicates the downstream process. The present study describes how this problem was tackled by media modification and mutation. Induced mutants were generated using UV and EMS as mutagenic agents followed by rational selection based on antibiotic resistance. On screening of these mutants in sucrose based PM-2 medium, UV induced, vancomycin resistant mutant M-6, showed significant reduction (6.29 fold) in viscosity development in the broth. Mutant M-6(S), a natural variant of mutant M-6, resistant to high substrate concentration was further selected for the CoQ 10 production. Cane molasses as carbon source was found to be best suitable for CoQ 10 fermentation using mutant M-6(S). Replacing sucrose with cheaper cane molasses significantly reduced the broth viscosity with improved specific CoQ 10 content, thereby generating cost effective fermentation process. The newly developed mutant strain produced 48.89 mg/L of CoQ 10 with specific CoQ 10 content of 1.87 mg/g of DCW at 25°C, 500 rpm agitation and 0.2 vvm aeration using continuous fed batch fermentation and newly formulated cane molasses medium.

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Kinetics of Agrobacterium tumefaciens ubiquinone-10 batch production

Cited by 34 publications

References 20 publications

Enhanced production of CoQ10 by newly isolated Sphingomonas sp. ZUTEO3 with a coupled fermentation–extraction process

Enhanced production of CoQ10 by newly isolated Sphingomonas sp. ZUTEO3 with a coupled fermentation–extraction process

Coenzyme Q10 production in a 150-l reactor by a mutant strain of Rhodobacter sphaeroides

Process and Strain Development for Reduction of Broth Viscosity with Improved Yield in Coenzyme Q10 Fermentation by Agrobacterium tumefaciens ATCC 4452

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