Kinetics of Antibody Response to Polypeptides of Pathogenic and Nonpathogenic Treponemes in Experimental Syphilis

Wicher, Konrad; Wos, Susan M.; Wicher, Victoria

doi:10.1097/00007435-198610000-00008

Cited by 11 publications

(5 citation statements)

References 29 publications

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“…Serum immunoglobulin G (IgG) and IgM responses to T. pallidum proteins in syphilis patients and in experimentally infected animals have been studied extensively by both Western blot (12,41,50,68,69,105,112,113,153,227,242,243) and radioimmunoprecipitation (4,17,18,133,195,198,218) techniques. Although it is difficult to correlate the results of these studies precisely, the following general observations appear to be consistent.…”

Section: Antibody Responsesmentioning

confidence: 99%

“…Similar results have been obtained in time course experiments with rabbits, guinea pigs, and hamsters infected with T. pallidum subsp. pallidum or other T. pallidum subspecies (4,64,67,112,113,239,242,243). Successful treatment of syphilis causes a gradual diminution of anti-T. pallidum antibodies, particularly the IgM response (113).…”

Section: Antibody Responsesmentioning

confidence: 99%

See 1 more Smart Citation

Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

Norris

1993

Microbiological Reviews

116

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Section: Antibody Responsesmentioning

confidence: 99%

Section: Antibody Responsesmentioning

confidence: 99%

Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

Norris

1993

Microbiological Reviews

116

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“…pallidum is a spirochaetal bacterium that causes venereal syphilis. Experimental infection of rabbits closely resembles primary infection in humans and is characterized by an early infiltration of T lymphocytes and macrophages into the lesion site [1][2][3] with concurrent development of specific antibodies [4][5][6][7]. Based on histological studies [8][9][10][11], rapid clearance of treponemes from lesions is thought to be the result of active phagocytosis and dissolution in macrophages.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the serum requirement for macrophage-mediated killing of Treponema pallidum ssp. pallidum: Relationship to the development of opsonizing antibodies

Baker-Zander¹

1993

FEMS Immunology and Medical Microbiology

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Serum pools were collected from rabbits bled at various times after intra-testicular infection with Treponema pallidum ssp. pallidum. These were tested for their ability to opsonize T. pallidum and promote killing of the organisms by macrophages. Compared to normal sera, significant opsonization was first seen on day 10 of infection as measured by both ingestion (P < 0.001) and macrophage-mediated killing (P = 0.006); significant levels of functional antibodies persisted through 300 days of infection. Although opsonic activity peaked early in infection, antibodies that promoted optimal macrophage-mediated killing developed much later, suggesting that these two functions may represent activities of antibodies with differing specificities or affinities. The initial development of antibodies that augment both phagocytosis and killing corresponds with the in vivo clearance of treponemes from the primary site of infection. These observations support the hypothesis that macrophages are the major effector mechanism for elimination of T. pallidum during early syphilis infection.

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“…The Western blot (WB) method has been used for the last 15 years to investigate the immune response to individual Treponema pallidum antigens in sera from experimentally infected animals (1,16,25,26,43,46) and from humans with naturally occurring syphilis (4,5,6,10,17,28,30,40,47,49). This method has been proposed as a possible alternative to either FTA-ABS or MHA-TP for the confirmation of the serological diagnosis of syphilis.…”

mentioning

confidence: 99%

“…This method has been proposed as a possible alternative to either FTA-ABS or MHA-TP for the confirmation of the serological diagnosis of syphilis. At least nine T. pallidum polypeptides with apparent molecular masses of 15 (TpN15), 17 (TpN17), 33, 37 (TpN37), 39,43,45 (TmpA), 47 (TpN47), and 97 kDa have been identified as major immunogens (2,11,31,33,34,45,46). Among these polypeptides, at least five (TpN15, TpN17, TpN37, TmpA, and TpN47) proved to be of diagnostic relevance (4,23,28,31,32).…”

mentioning

confidence: 99%

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

Sambri¹,

Marangoni²,

Eyer³

et al. 2001

Clin Diagn Lab Immunol

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Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially cross-reactive) were tested to evaluate the diagnostic performance of recombinant Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) and T. pallidum hemagglutination (MHA-TP) for the laboratory diagnosis of syphilis. None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from patients with secondary and late syphilis and 94.6 and 98.6% with sera from patients with primary and latent syphilis, respectively). The overall sensitivity of recWB was 98.8% and the specificity was 97.1% with MHA-TP as the reference method. These values for sensitivity and specificity were slightly superior to those calculated for wclWB (sensitivity, 97.1%, and specificity, 96.1%). With wclWB as the standard test, the sensitivity and specificity of recWB were 98.9 and 99.3%, respectively. These findings suggest that the five recombinant polypeptides used in this study could be used as substitutes for the whole-cell lysate T. pallidum antigens and that this newly developed recWB test is a good, easy-to-use confirmatory method for the detection of syphilis antibodies in serum.

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Kinetics of Antibody Response to Polypeptides of Pathogenic and Nonpathogenic Treponemes in Experimental Syphilis

Cited by 11 publications

References 29 publications

Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

Polypeptides of Treponema pallidum: progress toward understanding their structural, functional, and immunologic roles. Treponema Pallidum Polypeptide Research Group.

Characterization of the serum requirement for macrophage-mediated killing of Treponema pallidum ssp. pallidum: Relationship to the development of opsonizing antibodies

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

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