Kinetics of antifungal activity of home-generated ozonated Water on Candida albicans

Abstract

Background and Purpose: Candida-associated denture stomatitis is one of the most common forms of oral candidiasis among denture wearers. Regarding this, the aim of the present study was to evaluate the antifungal effects of home-generated ozonated water on the adhesion of the C. albicans attached to the surface of the denture base acrylic resins.Materials and Methods:For the purpose of the study, different concentrations of C. albicans were added to the tubes containing acrylic resin blocks, and then incubated for 2 h at 35°C. The samples were assigned into three groups, each of which contained 42 samples, including normal saline (NS) solution as the negative control, nystatin (N) solution as the positive control, and ozonated water as the test group. The samples were washed and placed in an ultrasonic bath. Subsequently, the saline solution was cultured on Sabouraud dextrose agar. The concentrations of Candida were evaluated during the contact times.Results:The test group (i.e., ozonated water) with 114 colony-forming units (CFU) showed a significant reduction of Candida colonies, compared to the NS group with 2,172 CFU. The 120- and 1-minute incubation with ozonated water showed the highest and lowest effects on the viability of Candida adhered to the acrylic resin, respectively. Conclusion:Based on the findings, home-generated ozonated water can be applied to remove the Candida attached to the surface of the denture plates.

Comparison of Virulence Factors of Different Candida Species Isolated from the Oral Cavity of Cancer Patients and Normal Individuals

“…Infections caused by Candida species are increasing in immunocompromised patients. Oral candidiasis is one of the most common opportunistic infections in this group of patients (29)(30)(31). The main cause of candidiasis is C. albicans although non-albicans such as C. glabrata and C. krusei are increasing (32).…”

Effect of Ozone and Two Common Denture Cleaners on Tensile Bond Strength and Surface Hardness of a Silicone Soft Liner

“…These devices are available, relatively cheap, and easy to use and require no special training. In a recent study, the anti-fungal efficacy of this device was tested, and the results revealed that with enough exposure time (80 minutes), the colony count drops significantly [20]. The data regarding the effect of ozonated water on the mechanical properties of denture soft lining materials are scarce.…”

Successful treatment with oral methylprednisolone and cyclosporine for refractory pyoderma gangrenosum in children: Report of a case and review of the literature

“…Topical government of PG is required to contribute to the healing of ulcers. About 5% potassium permanganate solution and ozonated water have been reported as effective treatment methods for certain types of wounds . We had no idea why the lesion was in progress before he came to us, although with prednisolone over 1 mg/kg/d for more than 1 month.…”

“…About 5% potassium permanganate solution and ozonated water have been reported as effective treatment methods for certain types of wounds. 11,12 We had no idea why the lesion was in progress before he came to us, although with prednisolone over 1 mg/kg/d for more than 1 month. We considered that besides systemic treatment, we actually strengthened measures for wound care.…”