Kinetics of Bacterial Fluorescence Staining with 3,3′-Diethylthiacyanine

Thomas, Marlon S.; Nuñez, Vicente; Upadhyayula, Srigokul; Zielins, Elizabeth R.; Bao, Duoduo; Vasquez, Jacob M.; Bahmani, Baharak; Vullev, Valentine I.

doi:10.1021/la1013279

Cited by 20 publications

(31 citation statements)

References 87 publications

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“…The data analysis was conducted using IgorPro v. 6 installed on MacOS and Windows workstations (Thomas et al 2010c;Jones and Vullev 2002;Vullev and Jones 2002;Wan et al 2008;Bao et al 2009;Jones et al 2007). …”

Section: Examination Of Qa Semi-quantitativelymentioning

confidence: 99%

Dependence of the quality of adhesion between poly(dimethylsiloxane) and glass surfaces on the composition of the oxidizing plasma

Chau

Millare

Lin

et al. 2010

Microfluid Nanofluid

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Controlled surface oxidation of polydimethylsiloxane (PDMS) is essential for permanent adhesion between device components composed of this elastomer. The permanent adhesion between such microdevice components results from covalent crosslinking across the interfaces between PDMS and other silica-based materials, such as glass, quartz, and PDMS. Optimal duration and conditions of oxidation, attained via treatments with oxygen-containing plasma, are crucial for microfabrication procedures with quantitative yields. While insufficient PDMS oxidation does not provide high enough surface density of siloxyl groups for cross-interface linking, overoxidation of PDMS yields rough silica surface layers that prevent the adhesion between flat substrates. Ideally, for a set of plasma conditions, the range of treatment durations producing permanent adhesion should be as broad as possible: i.e., the surface oxidation of PDMS sufficient for irreversible binding has to complete significantly before the effects of overoxidation become apparent. Such a requirement assures that relatively small fluctuations in the treatment conditions will not result in over-or under-oxidation and, hence, will not compromise the yields of the fabrication procedures. We examined the dependence of the quality of adhesion (QA) between plasma-treated PDMS and glass substrates on the composition of the oxygen-containing plasma and on the radio frequency (RF) of the plasma generator. We observed that plasma generated at megahertz RF provided superior conditions than kilohertz RF. Concurrently, an increase in the oxygen content of binary gas mixtures, used for the plasma, broadened the treatment durations that afford superior QA.

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Section: Examination Of Qa Semi-quantitativelymentioning

confidence: 99%

Dependence of the quality of adhesion between poly(dimethylsiloxane) and glass surfaces on the composition of the oxidizing plasma

Chau

Millare

Lin

et al. 2010

Microfluid Nanofluid

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“…1a, b) [10,12]. We recorded the kinetics of fluorescence staining with ThT using protocols developed in our lab [11].…”

Section: Methodsmentioning

confidence: 99%

“…The timed progression of staining, monitored via the enhancement of the fluorescence of the staining dye, differed for different species, which, in turn, provided venues for statistically significant discernibility among microbial organisms [11]. Subsequently, we previously demonstrated the completion of mixing for this experimental setup in less than 1-2 seconds [11].…”

mentioning

confidence: 87%

“…Recently, we demonstrated that the kinetics of fluorescence enhancement of cyanine stains, when applied to different vegetative bacteria, are characteristic of the species, regardless of whether the bacteria are Gram positive or Gram negative [11]. The timed progression of staining, monitored via the enhancement of the fluorescence of the staining dye, differed for different species, which, in turn, provided venues for statistically significant discernibility among microbial organisms [11].…”

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confidence: 99%

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Dynamic staining of Bacillus endospores with Thioflavin T

Upadhyayula

Lam

et al. 2012

2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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Rapid detection and identification of endospores presents a range of complex challenges. Dynamic staining approach, developed in our lab, utilizes the time-course fluorescence enhancement of an amyloid-staining dye, Thioflavin T (ThT), after mixing with intact endospores. We examined the kinetics of staining Bacillus atrophaeus and Bacillus thuringiensis endospores, and the rates of staining were different for the two bacilli when intact endospores were treated with ThT. This finding demonstrates an avenue for attaining information about the sporulated bacterial species without lysing, germinating or other pretreatment steps.

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“…9), using a solution of coumarin 151 in ethanol as a standard [170]. C NMR spectra were recorded with complete proton decoupling.…”

Section: Experimental Materials General Synthesis Informationmentioning

confidence: 99%

Bioinspired approach toward molecular electrets: synthetic proteome for materials

Espinoza

Larsen-Clinton

Krzeszewski

et al. 2017

Pure and Applied Chemistry

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Molecular-level control of charge transfer (CT) is essential for both, organic electronics and solarenergy conversion, as well as for a wide range of biological processes. This article provides an overview of the utility of local electric fields originating from molecular dipoles for directing CT processes. Systems with ordered dipoles, i.e. molecular electrets, are the centerpiece of the discussion. The conceptual evolution from biomimicry to biomimesis, and then to biological inspiration, paves the roads leading from testing the understanding of how natural living systems function to implementing these lessons into optimal paradigms for specific applications. This progression of the evolving structure-function relationships allows for the development of bioinspired electrets composed of non-native aromatic amino acids. A set of such non-native residues that are electron-rich can be viewed as a synthetic proteome for hole-transfer electrets. Detailed considerations of the electronic structure of an individual residue prove of key importance for designating the points for optimal injection of holes (i.e. extraction of electrons) in electret oligomers. This multifaceted bioinspired approach for the design of CT molecular systems provides unexplored paradigms for electronic and energy science and engineering.

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Kinetics of Bacterial Fluorescence Staining with 3,3′-Diethylthiacyanine

Cited by 20 publications

References 87 publications

Dependence of the quality of adhesion between poly(dimethylsiloxane) and glass surfaces on the composition of the oxidizing plasma

Dependence of the quality of adhesion between poly(dimethylsiloxane) and glass surfaces on the composition of the oxidizing plasma

Dynamic staining of Bacillus endospores with Thioflavin T

Bioinspired approach toward molecular electrets: synthetic proteome for materials

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