Kinetics of Cellular Viability in Warm Versus Cold Ischemia Conditions of Kidney Preservation

Abstract

We have determined the kinetics of the cellular viability ratio (CVR), defined as the number of living cells over the total cell count, in pig kidneys using propidium iodide and fluorescein diacetate staining, as a function of time and preservation conditions. The kidneys were preserved in warm or cold ischemia in order to mimic the conditions of transplantation from non-heart-beating donors or multiple removal with optimal preservation of the graft, respectively. To determine the CVR, the cells were obtained by a fine-needle aspiration biopsy, which minimizes the damage to the graft. A biometric analysis by regression enabled the determination of the time dependence for warm ischemia (CVR(t) = 80.0 x e(-0.733-t)(+2.7/-0.36)) and for cold ischemia (CVR(t) = 80.0 x e(-0.022-t)(+1.57/-0.64)) with a confidence interval of 95%. These master curves allow us to predict, under the described conditions, the CVR after a given ischemia time. The half-life of the cells can be deduced from the time-dependent CVR(t), and is 0.64 hr (38 min) for warm ischemia and 21.4 hr for cold ischemia. Further, the CVR for a given kidney can be used to assess its condition at removal: if the CVR is below 48% at 2 hr after removal, one can conclude that the organ has suffered a period of warm ischemia.

Morphological and ultrastructural changes occurring during degeneration of goat preantral follicles preserved in vitro

“…Braun-Collins solution functions via hypothermic preservation in hyperosmotic solution, followed by a low cellular dehydration, thus avoiding consequent cell swelling. At greater temperatures, our results were similar to those obtained by Savioz et al (1996) with kidney preservation at 14°C. These authors demonstrated the lack of effectiveness of BraunCollins solution when its use was not associated with preservation at 4°C.…”

Effect of Braun-Collins and Saline solutions at different temperatures and incubation times on the quality of goat preantral follicles preserved in situ

“…Braun-Collins solution has been successfully used in preservation at 4°C of lung (Fukuse et al, 1996), liver (Adam et al, 1996), kidneys (Savioz et al, 1996) and heart (Demmy et al, 1997) tissues. At greater temperatures, our results were similar to those obtained by Savioz et al (1996) with kidney preservation at 14°C. These authors showed the low efficiency of Braun-Collins solutions when its use was not associated with the hypothermic principle.…”

“…Saline solution (0.9%) is commonly used as a conservation media for ovaries during short periods of time (Solano et al, 1994, Schernthaner et al, 1997and Azambuja et al, 1998, although this solution has not been tested as a medium for preantral follicle storage for longer periods. Braun-Collins solution is another medium that has been successfully used in the preservation of different organs, such as lungs (Fukuse et al, 1996), liver (Adam et al, 1996), kidney (Savioz et al, 1996) and heart (Demmy et al, 1997).…”

Influence of Cold Storage on Renal Ischemia Reperfusion Injury after Non-Heart-Beating Donor Explantation

“…For organ preservation and transplantation, it is extremely important to define the degree of ischemia reperfusion injury [3]. The circumstances of explantation, ischemic periods and the preservation solution are known to be highly important factors with regard to organ quality not only for the kidney [4, 5]. In general, 30–60 min ischemia time to explant kidneys from NHBD is considered to represent the maximum time limit for viable transplantation [4, 6].…”