Kinetics of chromate reduction during naphthalene degradation in a mixed culture

Shen, H. Z.; Pritchard, P. H.; Sewell, Guy W.

doi:10.1002/(sici)1097-0290(19961105)52:3<357::aid-bit1>3.0.co;2-j

Cited by 16 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After operating the reactors under oxygen stressed con- (Shen et al 1996), Bacillus cereus, Bacillus thirungiensis (Francisco et al 2002;Camargo et al 2003;Faisal & Hasnain 2006), and…”

Section: Characterisation Of Microcosm Bacteria (After 15 Days)mentioning

confidence: 99%

Microbial culture dynamics and chromium (VI) removal in packed-column microcosm reactors

Molokwane

Chirwa

2009

Water Science and Technology

View full text Add to dashboard Cite

Microbial Cr(VI) reduction in groundwater aquifer media was investigated in microcosm reactors extracted from Cr(VI) contaminated sites in South Africa. The reactors were operated under an influent Cr(VI) concentration of 40 mg/L to simulate the current Cr(VI) level at the contaminated site. Near complete Cr(VI) removal was observed in microcosm reactors inoculated with Cr(VI) reducing bacteria from dried activated sludge collected from a treatment plant receiving periodic loadings of Cr(VI). The best performance was observed under low hydraulic loading (flow rate, Q=0.310 cm(3)/hr). Microbial culture characterisation results showed a change in culture composition after 17 days of reactor operation, indicating Bacillus and Lysinibacillus species as the most dominant organisms in reactors that reduced Cr(VI). The predominance of Bacillus and Lysinibacillus species was either due to resilience against toxicity or adaptation to the changing conditions in the reactor. This research was the initial step towards the development of an in situ bioremediation process to contain the spread of a Cr(VI) plume in a groundwater aquifer at contaminated site in Brits, South Africa. South Africa holds about 72% percent of the world's chromium resources, the majority of which is mined in the North Eastern region of the country formally known as Transvaal.

show abstract

“…After operating the reactors under oxygen stressed con- (Shen et al 1996), Bacillus cereus, Bacillus thirungiensis (Francisco et al 2002;Camargo et al 2003;Faisal & Hasnain 2006), and…”

Section: Characterisation Of Microcosm Bacteria (After 15 Days)mentioning

confidence: 99%

Microbial culture dynamics and chromium (VI) removal in packed-column microcosm reactors

Molokwane

Chirwa

2009

Water Science and Technology

View full text Add to dashboard Cite

show abstract

“…Cr(VI) reduction was observed throughout this ORP range by Wang et al 20 , who also found no correlation between Cr(VI) reduction and the ORP values of the culture medium. No definite redox potential requirement has been made for bacterial Cr(VI) reduction as varying ORP requirements with different bacteria have been found 21,22 . The U(VI) tolerance and reduction by Thermus SA-01 was also determined during aerobic growth by varying the concentration of U(VI).…”

Section: Metal Reduction During Growthmentioning

confidence: 99%

Metabolic promiscuity from the deep subsurface: a story of survival or superiority

et al. 2008

View full text Add to dashboard Cite

The Witwatersrand Supergroup is a 2.9-billion-year-old formation of low permeability sandstone and shale with minor volcanic units and conglomerates with an ambient rock temperature of approximately 60°C. Thermus scotoductus SA-01 was isolated from fissure water at a depth of 3.2 kmbls in a South African gold mine and it shows the ability to reduce a variety of heavy metals under anaerobic conditions. It has been postulated that such microorganisms could play an important role in nutrient and metal cycling within the subsurface. Recently, our studies indicate that the cycling of metals could also occur under aerobic conditions and not only by the action of redox active enzymes, but other diverse metabolic proteins as well.In this study the capability of specific proteins to interact with metals is elucidated. Using Thermus SA-01 and its now completed genome sequence, metal reduction is studied through classic proteomic-and genomic methods. Finally we identify thermostable enzymes responsible for the transformation of various metals (Iron, Chrome, Uranium, Gold, etc) and discuss that reduction occurs via the serendipitous action of enzymes with other primary physiological functions, some of which are classical catabolic enzymes and anabolic proteins. This paper discusses the use of a ubiquitous enzyme/protein performing more than one function, possibly detoxifying the environment and using moonlighting as resource to decrease cellular energy requirements rather than elaborate metabolism in the subsurface.

show abstract

“…The amount of Cr(VI) reduction was 1.0-1.5% for the abiotic experiment, 3.9-4.5% for biotic, and 7.2% for biotic in the presence of dissolved toluene. A. oxydans can aerobically reduce Cr(VI) at a faster pace during concomitant biodegradation of volatile organic compoundssuch as toluene.Other bacteria with similar abilitiesto detoxify mixtures of Cr(VI) and organic compounds have been reported elsewhere (Shen et al 1996).…”

Section: Identi Cation Of Cultivable Cr(vi)-tolerant and -Reducing Enmentioning

confidence: 99%

“…Recent studies have con rmed that certain species of bacteria (Wang and Shen 1995;Turick et al 1996;Lovley and Coates 1997;Silver 1998), molecules of mixed mineral oxides [such as Fe(II), Fe(III), Mn(II), Mn(III), and Mn(IV)], and many small organic compounds can catalyze the reduction of Cr(VI) to Cr(III) (Bidoglio et al 1993;Deng and Stone 1996;Guo et al 1997). This has stimulated keen interest in the exploration of the biogeochemical reduction of the Cr(VI) metal ions in geologic materials (e.g., Losi et al 1994;Shen et al 1996;Ahmann, 1997) to obtain insights into the selection and improvement of remediation strategies to immobilize and detoxify Cr(VI) . Downloaded by [University of Saskatchewan Library] at 12:10 03 January 2015…”

mentioning

confidence: 99%

“…Arthrobacter oxydans, a widespread Gram-positive endolithic bacterium found in our geologicsamples (see below), was used to model Cr(VI)-tolerant and -reducing bacteria. Toluene vapor was selected as a model organic co-contaminant,because this type of small aromatic hydrocarbon is commonly found as a co-contaminant in many Cr-contaminated sites (Shen et al 1996). At the end of the SR-FTIR spectromicroscopy study, the nal spatial distribution of chromium on surfaces of geologic samples was validated by micro-x-ray uorescence (l -XRF) spectroscopy.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Real-Time Characterization of Biogeochemical Reduction of Cr(VI) on Basalt Surfaces by SR-FTIR Imaging

Holman¹,

Perry²,

Martin³

et al. 1999

Geomicrobiology Journal

View full text Add to dashboard Cite

Kinetics of chromate reduction during naphthalene degradation in a mixed culture

Cited by 16 publications

References 12 publications

Microbial culture dynamics and chromium (VI) removal in packed-column microcosm reactors

Microbial culture dynamics and chromium (VI) removal in packed-column microcosm reactors

Metabolic promiscuity from the deep subsurface: a story of survival or superiority

Real-Time Characterization of Biogeochemical Reduction of Cr(VI) on Basalt Surfaces by SR-FTIR Imaging

Contact Info

Product

Resources

About