“…To minimize introduction of pathogens into the laboratory environment, researchers often use synthetic wastewaters in laboratory treatment studies (Cao et al, 1992; Chen et al, 1995; Kargi and Karapinar, 1995; Kuai and Verstraete, 1998; Noto et al, 1998; Raj and Murphy, 1998; Rodgers, 1999; Romanski et al, 1997; Yoo et al, 1999). Laboratory chemicals were combined to develop a synthetic wastewater with characteristics similar to typical septic tank effluent (Brooks et al, 1984; Crites and Tchobanoglous, 1998; Kristiansen, 1981; Piluk and Hao, 1989; Rock et al, 1984; USEPA, 1977, 2002).…”