Kinetics of degradation of carbendazim by B. subtilis strains: possibility of in situ detoxification

Salunkhe, Varsha P.; Sawant, Indu S.; Banerjee, Kaushik; Wadkar, Pallavi N.; Sawant, Sanjay D.; Hingmire, Sandip

doi:10.1007/s10661-014-4027-8

Cited by 34 publications

(15 citation statements)

References 22 publications

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“…when cultured at a concentration of 250 μg mL -1 carbendazim showed the best growth rates ( Figure 1). Zhang et al (2014) Fang et al (2010), Zhang et al (2013), and Salunkhe et al (2014) showed that bacterial strains used in biodegradation tests can be isolated using carbendazim as the only source of carbon. According to Fang et al (2010), such characteristics are required when isolating microorganisms to be used for bioremediation purposes.…”

Section: Resultsmentioning

confidence: 99%

“…(Xiao et al, 2013), Bacillus subtilis TL-171 (Salunkhe et al, 2014), and Brevibacillus borstelensis (Arya & Sharma, 2015). Salunkhe et al (2014) reported that four strains of B. subtilis individually degraded from 75.7 to 95.2% MBC in a concentration of 10 μg mL -1 after 15 days of incubation. The concentration of MBC used to validate the degradation potential by the bacterial isolates Stenotrophomonas sp.…”

Section: Resultsmentioning

confidence: 99%

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Biodegradation of the fungicide carbendazim by bacteria from Coriandrum sativum L. rhizosphere

Santos

Batista

Barroso

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. The biocidal agrochemicals commonly used in agriculture can remain in the soil, affecting the environmental conditions and causing serious risks to health. Knowing that soil microorganisms, especially those from the rhizosphere, can degrade environmental xenobiotics, it was evaluated the potential of bacteria isolated from Coriandrum sativum L. rhizosphere to biodegrade carbendazim (MBC), a fungicide extensively used by agriculturists from rural farming communities in Manaus, Amazonas. Cultures carried out in medium containing carbendazim as a sole carbon source enabled the isolation of 80 bacteria, in the established conditions. Assays to determine degradation potential allowed the selection of the two elite isolates identified as Stenotrophomonas sp. and Ochrobactrum sp. Quantitative assays with each strain individually or in consortium, were carried out using minimal salt medium added with carbendazim (250 μg mL ) and incubated at 30°C, under agitation (125 rpm) for 21 days. Samples used in the biodegradation test were HPLC analyzed for final fungicide quantitation. The Stenotrophomonas sp. strain was more efficient (68.9%) to degrade carbendazim and showed no toxicity in tests with Artemia salina.Keywords: Amazon soil, fungicide, coriander, carbendazim-degrading bacteria, Stenotrophomonas, xenobiotics.Biodegradação do agrotóxico carbendazim por bactérias da rizosfera de Coriandrum sativum L.RESUMO. Agrotóxicos são comumente utilizados na produção agrícola, podendo persistir no solo, afetar a qualidade do ambiente e causar sérios riscos à saúde. Sabendo-se que micro-organismos do solo, principalmente aqueles da rizosfera, podem degradar produtos xenobióticos avaliou-se o potencial de bactérias isoladas da rizosfera de Coriandrum sativum L. em degradar carbendazim, um fungicida usado extensivamente em comunidades de agricultores rurais em Manaus, Amazonas. Procedimentos de cultivo em meio, contendo carbendazim como única fonte de carbono, mostraram que 80 bactérias cresceram nas condições estabelecidas. Ensaios de eficiência de degradação permitiram a seleção dos dois melhores isolados que foram identificados como Stenotrophomonas sp. e Ochrobactrum sp. Os ensaios quantitativos, com cada cepa individualmente e com as duas em consórcio, foram conduzidos em meio mínimo contendo sais, acrescido de carbendazim (250 μg mL -1 ) e incubados a 30°C, 125 rpm, por 21 dias. A quantificação final do fungicida nas amostras do ensaio de biodegradação foi realizada em HPLC. A linhagem Stenotrophomonas sp. apresentou maior eficiência, degradando 68,9% do total de carbendazim e não apresentou toxicidade nos testes realizados com Artemia salina.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Biodegradation of the fungicide carbendazim by bacteria from Coriandrum sativum L. rhizosphere

Santos

Batista

Barroso

et al. 2017

Acta Sci. Biol. Sci.

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“…Zhang et al [42] report that the effectiveness of carbendazim degradation by B. pumilus NY97-1 at concentrations of 10, 30, 50, 100, and 300 mg/L was 42.44%, 48.97%, 77.19%, 78.66%, and 90.07%, respectively. Salunkhe et al [43] report that the degradation of carbendazim by four B. subtilis strains, TL-171, TS-204, DR-39 and CS-126, was within the range of 75.7%-95.2%. A degradation effectiveness of 73.2% was also observed by Panda et al [44] for B. licheniformis JTC-3 bacteria.…”

Section: Discussionmentioning

confidence: 99%

Influence of Bacillus subtilis and Trichoderma harzianum on Penthiopyrad Degradation under Laboratory and Field Studies

et al. 2020

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In plant protection, biological preparations are used alternately with chemical pesticides. The applied microorganism can influence the concentration of chemical substances. Laboratory and field studies were conducted to assess the influence of Bacillus subtilis and Trichoderma harzianum on the penthiopyrad concentration. In laboratory studies, the effectiveness of penthiopyrad degradation by B. subtilis was approximately 5% during 14 days of the experiment. For penthiopyrad treated with T. harzianum strains, the degradation effectiveness ranged from 34.2% on Day 3 to 56.9% on Day 14. In experiments testing the effects of mixed culture of microorganisms, the effectiveness of penthiopyrad degradation ranged from 23.7% on Day 3 to 29.1% on Day 14. After treatment of apple trees of Gala and Golden Delicious varieties with a biological preparation, a maximum degradation of penthiopyrad of 20% was found in both varieties. Samples of apples were prepared by the quick, easy, cheap, effective, rugged and safe (QuEChERS) method, and penthiopyrad was analyzed by gas chromatography with a mass detector. A determined value of the chronic exposure to penthiopirad was 1.02% of the acceptable daily intake, both for children and for adults. The acute exposure amounted to 7.2% and 1.9% of the acute reference dose for children and adults, respectively. These values were considered to be acceptable and not threatening to health.

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“…Some strains of Bacillus even facilitates as plant growth accelerant or antifungal biopesticide [20][21][22] . Therefore, the application of Car4 combined with several Bacillus strains is feasible in MBC degradation and probiotic enrichment [10,11] . However, MBC functions as an antibiotic in controlling plant disease, and bacterial bioremediation is invalid when MBC concentration is high.…”

Section: Discussionmentioning

confidence: 99%

“…In previous researches, MBC-degrading strains were isolated such as Microbacterium and Ralstonia. In contrast, there is only a few literatures reported the MBC-degrading strain that belongs to Bacillus genus [10,11] . The advantage of using B. amyloliquefaciens over the other strains was that B. amyloliquefaciens was a defined agricultural probiotic, meaning that it can be used directly in soil bioremediation [12,13] .…”

Section: Isolation and Characterization Of Mbc-degrading Strainmentioning

confidence: 99%

Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food

Liu¹,

Chi²,

Ge³

2019

International Journal of Agricultural and Biological Engineering

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Carbendazim (MBC) is an effective antifungal and antibacterial pesticide in agricultural applications. However, the MBC-contaminated soil and food are difficult to be restored. In this work, a novel MBC-hydrolase HY-1 encoded by gene hy-1 from an isolated MBC-degrading bacteria Bacillus amyloliquefaciens has been screened and identified. The 858 bp hydrolase gene was expressed in E. coli BL21 and the 32 kDa hydrolase HY-1 was purified. The purified HY-1 was able to catalyze MBC into 2-aminobenzimidazole (2-AB) without the need for any cofactors. Then bioremediation experiment was conducted and both the strain Car4 and cell crude extract of E. coli (pET-hy1) accelerated MBC degradation in soil. Moreover, purified HY-1 was available in removing MBC residue on the surface of cucumber. This work explored the possibility of microbial and enzymatic bioremediation on MBC-contaminated soil and food, provide a new way for bioremediation of pesticide contaminations.

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Kinetics of degradation of carbendazim by B. subtilis strains: possibility of in situ detoxification

Cited by 34 publications

References 22 publications

<b>Biodegradation of the fungicide carbendazim by bacteria from <i>Coriandrum sativum</i> L. rhizosphere

<b>Biodegradation of the fungicide carbendazim by bacteria from <i>Coriandrum sativum</i> L. rhizosphere

Influence of Bacillus subtilis and Trichoderma harzianum on Penthiopyrad Degradation under Laboratory and Field Studies

Identification of a novel hydrolase encoded by hy-1 from Bacillus amyloliquefaciens for bioremediation of carbendazim contaminated soil and food

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