Kinetics of Enzyme Adsorbed on Adsorbent

SYMPOSIUM: Immobilized Enzymes in Food Systems

“…Glucoamylase also has been physically adsorbed or bound onto various matrices such as activated clay (Usami et al, 1968;Usami and Shirasaki, 1970) and activated charcoal (Usami and Shirasaki, 1970;Kimura et al, 1969). Glucoamylase, ionically bound t o DEAE cellulose, exhibited 1 6 t o 55% of the activity of the soluble enzyme but decreased temperature stability (Bachler et al, 1970).…”

Pilot plant production of glucose with glucoamylase immobilized to porous silica

“…The adsorbed enzyme showed a lower activity and lower temperature and pH stability than the free enzyme. Kimura et al (48) adsorbed glucoamylase on activated charcoal and acid clay. The enzyme was elutable, the increased, and the ^^nax decreased for the complex compared to the free enzyme.…”

The effect of slow substrate diffusion on the activity, stability, and selectivity of immobilized enzymes: a theoretical and experimental study

“…viii) Containment within semipermeable membrane devices. Glucoamylase was adsorbed on acid clay, activated charcoal, and diatomaceous earth and some of the adsorbed enzyme was descrbed from the surface (85,190). Many ion exchange resins such as Amberlite, Duolite, and derivatives of cellulose or Sephadex were also used as adsorbents ( Table 2).…”