Kinetics of fetal cellular and cell‐free DNA in the maternal circulation during and after pregnancy: implications for noninvasive prenatal diagnosis

Ariga, Hiromichi; Ohto, Hitoshi; Busch, Michael P.; Imamura, Shinya; Watson, Robert H.; Reed, William; Lee, Tzong‐Hae

doi:10.1046/j.1537-2995.2001.41121524.x

Cited by 248 publications

(172 citation statements)

References 62 publications

Supporting

Mentioning

162

Contrasting

Unclassified

Order By: Relevance

“…After delivery, the frequency of fetal microchimeric cells in the circulation rapidly decreases within 40 days (1). Multiple studies demonstrate that in a subset of women, fetal cells can persist for decades in blood (2) and specific organs (7,10,12,13,15).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The influence of fetal loss on the presence of fetal cell microchimerism: A systematic review

Khosrotehrani¹,

Johnson²,

Lau³

et al. 2003

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. Fetal cells enter the maternal circulation during most pregnancies. Their persistence for years occurs in only some women and has been associated with several autoimmune diseases such as systemic sclerosis. The objective of this study was to determine whether pregnancy history influences the persistence of fetal microchimeric cells.Methods. We reviewed all reports of studies on fetal cell microchimerism, defined as male DNA in maternal tissue, that describe individual pregnancy histories, disease diagnoses, and microchimerism status. The total numbers of pregnancies, births, and sons, the history of fetal loss (spontaneous abortion and elective termination), and the presence of a maternal autoimmune disease were tested as factors potentially associated with persistent microchimerism.Results. One hundred twenty-four subjects from 11 studies met the inclusion criteria. Only fetal loss was significantly associated with the presence of microchimerism (odds ratio 2.4, 95% confidence interval 1.2-6.0).Conclusion. These results suggest that fetomaternal cell trafficking following fetal loss may be important for the engraftment of microchimeric cells in maternal tissue. This may be due to an increased amount of fetomaternal transfusion or to transfer of a cell type that is more likely to engraft. We recommend that investigators in future studies on microchimerism report detailed pregnancy information, since these data are critical for the understanding of factors that influence the development of fetal cell microchimerism.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Most, if not all, pregnant women have fetal cells in their circulation (1). The persistence of these cells for decades after pregnancy has also been demonstrated (2).…”

mentioning

confidence: 99%

The influence of fetal loss on the presence of fetal cell microchimerism: A systematic review

Khosrotehrani¹,

Johnson²,

Lau³

et al. 2003

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…Fetal cells have been observed in the maternal circulation after human pregnancy [1] and persist there for decades [2] .…”

Section: Introductionmentioning

confidence: 99%

Fetal cell microchimerism in the maternal mouse spinal cord

Zhang

Zhao

et al. 2013

Neurosci. Bull.

View full text Add to dashboard Cite

Fetal cell microchimerism refers to the persistence of fetal cells in the maternal tissues following pregnancy. It has been detected in peripheral organs and the brain, but its existence in the spinal cord has not been reported. Our aim was to detect fetal cell microchimerism in the spinal cord of maternal mice. C57BL/6 female mice were crossed with GFP transgenic male mice and sacrificed after their first or third delivery. GFP-positive cells, which were presumably from fetuses whose fathers were GFP transgenic, were detected in the spinal cord by fl uorescence microscopy and immunohistochemistry. PCR was also performed to detect GFP DNA, which must come from GFP hemizygous fetuses. We found GFP-positive cells and detectable GFP DNA in most of the maternal spinal cords. Twenty percent (1/5) of the mice that were only pregnant once had detectable fetal cells, while 80% (4/5) of those that were pregnant three times had detectable fetal cells. Some fetal cells, which not only emitted green fluorescence but also expressed NeuN, were detected in the spinal cords from maternal mice. These results indicate that fetal cells migrate into the spinal cord of a maternal mouse during and/or after the gestational period, and the fetal cells may differentiate into neurons in the spinal cord.

show abstract

“…Previous studies have revealed that migration of fetal cells into maternal circulation starts during early gestation, and the frequency of fetal cell-associated DNA and cell free DNA in maternal blood increases with gestational age in humans. 7 In murine pregnancy both cellfree DNA and fetal cells have similarly been identified. 8,9 Migration of microchimeric fetal cells into maternal circulation before placenta formation Rei Sunami, Mayuko Komuro, Hikaru Tagaya and Shuji Hirata* Department of Obstetrics and Gynecology; University of Yamanashi; Chuo, Yamanashi Japan This manuscript has been published online, prior to printing.…”

mentioning

confidence: 99%

Migration of microchimeric fetal cells into maternal circulation before placenta formation

et al. 2010

View full text Add to dashboard Cite

Kinetics of fetal cellular and cell‐free DNA in the maternal circulation during and after pregnancy: implications for noninvasive prenatal diagnosis

Cited by 248 publications

References 62 publications

The influence of fetal loss on the presence of fetal cell microchimerism: A systematic review

The influence of fetal loss on the presence of fetal cell microchimerism: A systematic review

Fetal cell microchimerism in the maternal mouse spinal cord

Migration of microchimeric fetal cells into maternal circulation before placenta formation

Contact Info

Product

Resources

About