Kinetics of galactooligosaccharide (GOS) production with two β-galactosidases combined: Mathematical model and raw material effects

Rico-Rodriguez, Fabián; Noriega, M.A.; Lancheros, Ruth; Serrato-Bermúdez, Juan C.

doi:10.1016/j.idairyj.2021.105015

Cited by 8 publications

(4 citation statements)

References 28 publications

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“…1 but is mandatory for the assumption that each individual reaction step is elementary. Assuming homogeneous reaction conditions and no mass transfer limitation, the rate laws can be described by molecularity 8–10. Accordingly, there are nine reaction steps leading to nine single step frequencies and a comparatively extensive balancing of each component and enzyme species.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, modeling approaches are different and above all based on hardly comparable systems. Zhou et al 6 and Kim et al 7 used an approach derived from Michaelis‐Menten, while most publications describe a simplified power law approach wherein molecularity is assumed 8–10. Boon et al 11 even used an approach for multistep reactions according to King and Altman 12.…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides

Mueller

Runne

Hamel

2022

Chemie Ingenieur Technik

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The galacto-oligosaccharide synthesis by b-galactosidase from Bacillus circulans is described mathematically using three kinetic models of different complexity -a simple power law approach, an extended Michaelis Menten kinetics and, in particular, an advanced, innovative mechanistic kinetic model for biocatalysis. Based on a detailed experimental study, a comprehensive reaction network is identified, and a reliable database provided. The evaluation of the kinetic models studied is performed by the Akaike information criterion, which allows the assessment of the model accuracy taking into account complexity and transferability.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides

Mueller

Runne

Hamel

2022

Chemie Ingenieur Technik

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“…When cheese whey was used, respective maximum GOS yields were 35.6 and 29.6% of total carbohydrates. Additionally, they reported that although the combination of the two enzymes resulted in similar GOS yields to those obtained when the two β-galactosidases were studied separately, there were significant differences in the profile of the GOS products regarding their degree of polymerization [32].…”

Section: Comparison Of Galacto-oligosaccharide Profile Produced By Th...mentioning

confidence: 97%

Production of Prebiotic Galacto-Oligosaccharides from Acid Whey Catalyzed by a Novel β-Galactosidase from Thermothielavioides terrestris and Commercial Lactases: A Comparative Study

Limnaios,

Tsevdou,

Tsika

et al. 2023

Catalysts

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The steadily increasing global popularity of Greek strained yoghurt has necessitated alternative valorization approaches for acid whey, the major straining process effluent. In this context, prebiotic galacto-oligosaccharides can be enzymatically synthesized from acid whey lactose, via either commercial or novel β-galactosidases. A comparative study of galacto-oligosaccharide production from acid whey was carried out, employing two commercial β-galactosidases (from Kluyveromyces lactis and Aspergillus oryzae) and one novel, in-house produced (from Thermothielavioides terrestris), as a function of the initial lactose content and enzyme load. Selected reaction conditions for β-galactosidases from K. lactis, A. oryzae, and T. terrestris were 35 °C at pH 7.2, 45 °C at pH 4.5, and 50 °C at pH 4.0, respectively. Maximum galacto-oligosaccharide yields equal to 23.7, 23.4, and 25.7% were achieved with, respectively, 0.13 U/mL of K. lactis β-galactosidase in non-concentrated acid whey, 4 U/mL of A. oryzae β-galactosidase, and 8 U/mL of T. terrestris β-galactosidase in acid whey concentrated to 20% w/v initial lactose content. The increased galacto-oligosaccharide productivity of the thermophilic β-galactosidase from T. terrestris can be a determining asset in a combined concentration and oligomerization industrial process. This will allow for high galacto-oligosaccharide yields for efficient, cost-effective production of valuable prebiotics from acid whey.

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“…In the whey, the respective maximum y GOS values were 35.6 and 29.6% of the total sugars. Furthermore, it was noted that the simultaneous use of the enzymes yielded GOS quantities comparable to those obtained when the enzymes were used individually, yet there were notable distinctions in the GOS product profiles in terms of their polymerization degree[32].…”

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confidence: 99%

Cheese and Yogurt By-Products as Valuable Ingredients for the Production of Prebiotic Oligosaccharides

Limnaios,

Tsevdou,

Zafeiri

et al. 2024

Dairy

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The growing global market of dairy products has led to the need for alternative approaches regarding whey valorization, which is the primary by-product of cheese and strained yogurt production. In this context, prebiotic galactooligosaccharides can be produced enzymatically from whey using commercially available β-galactosidases. A comparative study was conducted to assess the production of galactooligosaccharides from sweet and acid whey, thereby employing two commercial β-galactosidases from Aspergillus oryzae and Kluyveromyces lactis. The study considered the initial lactose content and enzyme load as variables. The maximum yields of galactooligosaccharides in concentrated sweet whey (15% w/v initial lactose) and raw acid whey (3.1% w/v initial lactose) reached 34.4 and 14.7% with lactase from Kluyveromyces lactis (0.13 U/mL), respectively. The corresponding galactooligosaccharide yields for lactase from Aspergillus oryzae were equal to 27.4 and 24.8% in the most concentrated sweet and acid whey, respectively, using enzyme loads of 2 U/mL in sweet whey and 1 U/mL in acid whey. Concerning the profile of the produced galactooligosaccharides, the Kluyveromyces lactis lactase hydrolyzed lactose more rapidly and resulted in higher levels of allolactose and lower levels of 6-galactosyl-lactose, compared to the lactase from Aspergillus oryzae, and achieved in both cases a polymerization degree of up to six.

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Kinetics of galactooligosaccharide (GOS) production with two β-galactosidases combined: Mathematical model and raw material effects

Cited by 8 publications

References 28 publications

Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides

Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides

Production of Prebiotic Galacto-Oligosaccharides from Acid Whey Catalyzed by a Novel β-Galactosidase from Thermothielavioides terrestris and Commercial Lactases: A Comparative Study

Cheese and Yogurt By-Products as Valuable Ingredients for the Production of Prebiotic Oligosaccharides

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