1985
DOI: 10.1128/iai.49.2.452-454.1985
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Kinetics of growth and toxigenicity of Clostridium botulinum in experimental wound botulism

Abstract: An animal model of wound botulism was developed in mice using an inoculum of Clostridium botulinum type A spores. The number of C. botulinum in infected wounds was quantitated by culturing on egg yolk agar, and the level of C. botulinum toxin in infected wound tissue was measured by a bioassay in mice and by an enzyme-linked immunosorbent assay. All infected mice receiving no further treatment developed neuroparalytic symptoms consistent with botulism after an incubation period of ca. 48 h, and all of these an… Show more

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Cited by 12 publications
(3 citation statements)
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“…They included five type A (A-21, A-22, A-28, ATCC 3502 Hall, and ATCC 19397) and five proteolytic type B (B-35, B-42, B-45, 208 Bean, and ATCC 25765) strains. C. botulinum spores prepared by a conventional procedure (4) were distributed into small vials and kept frozen at -70°C. The number of spores present in frozen-thawed vials was then measured by viable plate count (4).…”
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confidence: 99%
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“…They included five type A (A-21, A-22, A-28, ATCC 3502 Hall, and ATCC 19397) and five proteolytic type B (B-35, B-42, B-45, 208 Bean, and ATCC 25765) strains. C. botulinum spores prepared by a conventional procedure (4) were distributed into small vials and kept frozen at -70°C. The number of spores present in frozen-thawed vials was then measured by viable plate count (4).…”
mentioning
confidence: 99%
“…C. botulinum spores prepared by a conventional procedure (4) were distributed into small vials and kept frozen at -70°C. The number of spores present in frozen-thawed vials was then measured by viable plate count (4). By mixing equal numbers of the five type A spores together and the five type B spores together, we prepared two separate spore mixtures which were used for inoculation.…”
mentioning
confidence: 99%
“…Nastaje kao posledica proizvodnje BoNT, in vivo, a nakon kolonizacije povređenog tkiva sporama C. botulinum. Dovoljno je 10 spora C. botulinum tipa A da izazove botulizam rana kod miševa (46). Ovaj oblik botulizma se najčešće dovodi u vezu sa ranama od fizičkih povreda, operacija, korišćenja droge putem injekcija ili zapaljenja nazalnih puteva usled ušmrkavanja kokaina (32).…”
Section: Forme Bolestiunclassified