Kinetics of Heat Inactivation of Ribonucleic Acid of Tobacco Mosaic Virus

Ginoza, William

doi:10.1038/181958a0

Cited by 75 publications

(15 citation statements)

References 27 publications

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“…1), which is also reported for tobacco mosaic virus (Ginoza, 1958), Sindbis virus (Barnes et al, 1969) and baculovirus (Gotoh et al, 2008). The corresponding Arrhenius plot (Fig.…”

Section: Ajbbsupporting

confidence: 76%

Influence of Process Conditions on Measles Virus Stability

Weiss

Salzig

Röder

et al. 2013

American Journal of Biochemistry and Biotechnology

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Recombinant measles viruses are currently tested in clinical trials as oncolytic agent to be applied in cancer therapy. Contrary to their use as vaccine where 10 3 infectious virus particles per dose are needed, for cancer therapy 10 9 virus particles should be provided per dose. This leads to other challenges for the production process when compared to vaccine production. This study presents measles virus stability with regard to conditions during production and storage of the virus. Relevant process parameters such as temperature (4-37°C), pH (pH 4-11), conductivity (1.5 to 137.5 mS cm ) and oxygen partial pressure were analyzed. The infectivity of measles virus particles decreased highly at 37 and 32°C, while at 22 and 4°C it remained stable for several hours or even days, respectively. The thermal inactivation reactions followed first order kinetics and the thermodynamic parameters enthalpy and entropy were estimated. Towards changes in pH measles virus particles were very sensitive, while no inactivation could be observed with varying conductivity. Measles virus incubation at an oxygen partial pressure of 100% did not lead to any loss of infectivity. The results show which parameters should be considered and controlled strongly in the production process to further raise measles virus yields for the high amount needed in cancer therapy approaches.

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“…1), which is also reported for tobacco mosaic virus (Ginoza, 1958), Sindbis virus (Barnes et al, 1969) and baculovirus (Gotoh et al, 2008). The corresponding Arrhenius plot (Fig.…”

Section: Ajbbsupporting

confidence: 76%

Influence of Process Conditions on Measles Virus Stability

Weiss

Salzig

Röder

et al. 2013

American Journal of Biochemistry and Biotechnology

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“…9 The different behavior of the two kinds of nucleic acid is presumed to reflect the great difference in their configurations, DNA possessing in its native state a rigid, secondarily bonded, double-stranded helical structure,'0 while in RNA, the secondary structure, if any, is certainly less extensive.9 11 The original aim of this investigation was to study in detail the loss of activity of the biologically active DNA, or transforming principle, from Diplococcus pneumoniae in order to see to what extent the reportedly complex behavior of this DNA could be related to the simpler behavior characteristic of the ribonucleic acid that one of us had previously studied. 9 To this end, data were taken consisting of the results of quantitative measurements of the transforming activity of the DNA after various heat treatments in aqueous solution. The resulting curves of activity versus time of heating showed so many interesting features that the following rather extensive analysis was undertaken.…”

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confidence: 99%

Mechanisms of Inactivation of Deoxyribonucleic Acids by Heat

Ginoza

Zimm

1961

Proc. Natl. Acad. Sci. U.S.A.

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BIOCHEMISTRY: GINOZA AND ZIMM 639 small effects here. We find no difference in the sensitivity of four markers at subcritical temperatures, whereas the Tm's are different, as first reported by Roger and Hotchkiss4 and by Marmur and Doty.3 No striking effect has yet been found for dependence of subcritical sensitivity on molecular weight, but there is a dependence of the Tm's on molecular weight, which varies for different markers. This will be the subject of a future report. Summary.-The sensitivity of the transforming activity of a DNA preparation to heating at temperatures below the critical melting temperature depends strongly on the method of preparing the DNA. DNA deproteinized by a few cycles of shaking with chloroform-isoamyl alcohol showed concave-downward, concentration-dependent, survival curves. The same DNA, treated further with a single phenol extraction, exhibited exponential survival curves, which were concentrationindependent and more stable and were very similar to the previously observed curves for a phenol preparation from a different strain of pneumococcus. A fraction of the first preparation recovered from a CsCl gradient showed intermediate stability.

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“…Ginoza (4) showed that for TMV-RNA, the half-life (t1,2) during incubation at 55 C in 0.1 M phosphate buffer (pH 7.4) was 39 min as determined by the retained infectivity of the RNA and concluded that inactivation was caused by breaking one phosphodiester bond. Assuming that there are 5900 bonds per TMV-RNA molecule, the value of kTMV at 50 C in buffer E is 4.6 x 10-5 sec-1 which would predict a t1,2 of 25 min, very similar to that reported by Ginoza (4).…”

Section: Resultsmentioning

confidence: 99%

Molecular Integrity of Plant Ribosomal Ribonucleic Acid

Hepburn¹,

Ingle²

1976

Plant Physiol.

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Thermal denaturation of plant ribosomal RNA followed by gel fractionation shows that although a large percentage of molecules contain breaks in the polynucleotide chain, 25S and 18S RNAs do exist as unique molecular species. Values for the rate constant of hydrolysis under routine denaturing conditions are of the order of 10(-7) to 10(-8) sec(- 1) and these are shown not to be a result of ribonuclease activity. This high rate of hydrolysis and the use of insensitive fractionation procedures may account for the reported absence of a 25S rRNA molecule and its apparent conversion to a molecule similar in size to 18S RNA.

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Kinetics of Heat Inactivation of Ribonucleic Acid of Tobacco Mosaic Virus

Cited by 75 publications

References 27 publications

Influence of Process Conditions on Measles Virus Stability

Influence of Process Conditions on Measles Virus Stability

Mechanisms of Inactivation of Deoxyribonucleic Acids by Heat

Molecular Integrity of Plant Ribosomal Ribonucleic Acid

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