Kinetics of in vitro renin and angiotensin converting enzyme inhibition by chicken skin protein hydrolysates and their blood pressure lowering effects in spontaneously hypertensive rats

Onuh, John O.; Girgih, Abraham T.; Malomo, Sunday A.; Aluko, Rotimi E.; Aliani, Michel

doi:10.1016/j.jff.2015.01.031

Cited by 52 publications

(45 citation statements)

References 31 publications

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“…A previous report has suggested the importance of branched-chain amino acids and aromatic amino acids in potentiating renin-inhibitory properties of food protein-derived peptides [ 45 ]. Generally, renin inhibitory activity of the hydrolysates was higher than ACE-inhibitory activity, which is different from other reports that showed a reverse trend [ 9 , 12 , 22 , 46 ]. Overall, these results confirm previously published literature data that indicate peptide-dependent ACE-inhibitory potency are more likely to be different from that renin inhibition.…”

Section: Discussioncontrasting

confidence: 99%

Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates

et al. 2015

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The aim of this work was to produce antihypertensive protein hydrolysates through different forms of enzymatic hydrolysis (2% pepsin, 4% pepsin, 1% alcalase, 2% alcalase, 2% papain, and 2% pepsin + pancreatin) of hemp seed proteins (HSP). The hemp seed protein hydrolysates (HPHs) were tested for in vitro inhibitions of renin and angiotensin-converting enzyme (ACE), two of the enzymes that regulate human blood pressure. The HPHs were then administered orally (200 mg/kg body weight) to spontaneously hypertensive rats and systolic blood pressure (SBP)-lowering effects measured over a 24 h period. Size exclusion chromatography mainly showed a 300–9560 Da peptide size range for the HPHs, while amino acid composition data had the 2% pepsin HPH with the highest cysteine content. Fluorescence spectroscopy revealed higher fluorescence intensities for the peptides when compared to the unhydrolyzed hemp seed protein. Overall, the 1% alcalase HPH was the most effective (p < 0.05) SBP-reducing agent (−32.5 ± 0.7 mmHg after 4 h), while the pepsin HPHs produced longer-lasting effects (−23.0 ± 1.4 mmHg after 24 h). We conclude that an optimized combination of the fast-acting HPH (1% alcalase) with the longer-lasting HPHs (2% and 4% pepsin) could provide daily effective SBP reductions.

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Section: Discussioncontrasting

confidence: 99%

Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates

et al. 2015

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“…Las enfermedades cardiovasculares son la principal causa de muerte a nivel mundial 12 . Un tercio de la población mundial padece hipertensión arterial 13 .…”

Section: Sistema Cardiovascularunclassified

Efectos fisiológicos de los péptidos bioactivos derivados de las proteínas del lactosuero en la salud: Una revisión

et al. 2019

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proteínas del lactosuero las convierte en un sustrato ideal para la generación de péptidos con actividad biológica. La presente revisión tiene por objetivo analizar y discutir los efectos fisiológicos de los péptidos bioactivos derivados de las proteínas del lactosuero (PBDL) en la salud. Asimismo, este trabajo muestra detalladamente las estructuras químicas de las secuencias de los PBDL capaces de ejercer efectos favorables in vitro e in vivo e influir positivamente en los sistemas cardiovascular, endócrino e inmunológico. Sin embargo, las metodologías para generar PBDL de manera controlada, la dosificación y las concentraciones óptimas han sido poco exploradas. Por lo que es importante llevar a cabo investigación de frontera que permita avanzar el umbral del conocimiento vislumbrando la posibilidad de utilizar los PBDL como coadyuvantes en la prevención y tratamiento de enfermedades. Palabras clave: Beneficios a la salud; Péptidos bioactivos; Proteínas derivadas del lactosuero.

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“…The results suggest lack of strong synergistic effects in the protein hydrolyzate, hence membrane fractionation led to isolation of peptide groups with higher renin‐inhibitory activity than the hydrolyzate. Renin inhibition kinetics revealed that the chicken skin peptides inhibited enzyme activity through the mixed‐type mode, which indicates binding mainly to the enzyme‐substrate complex to prevent catalysis (Onuh, Girgih, Malomo, Aluko, & Aliani, ). Unlike the in vitro , oral administration to SHRs showed that the hydrolyzate was more effective at reducing SBP (up to −33 mm Hg) when compared to −21, −18, and −16 mm Hg for the 5, 3, and 1 kDa peptides, respectively (Onuh et al, ).…”

Section: Animal Protein Hydrolyzatesmentioning

confidence: 99%

“…Renin inhibition kinetics revealed that the chicken skin peptides inhibited enzyme activity through the mixed‐type mode, which indicates binding mainly to the enzyme‐substrate complex to prevent catalysis (Onuh, Girgih, Malomo, Aluko, & Aliani, ). Unlike the in vitro , oral administration to SHRs showed that the hydrolyzate was more effective at reducing SBP (up to −33 mm Hg) when compared to −21, −18, and −16 mm Hg for the 5, 3, and 1 kDa peptides, respectively (Onuh et al, ). However, plasma analysis showed no effect on renin level, which suggests that the observed SBP‐lowering effects of the chicken skin peptides may have been due to ACE inhibition (plasma level was decreased) or other unknown effects on the cardiovascular system (Onuh et al, ).…”

Section: Animal Protein Hydrolyzatesmentioning

confidence: 99%

Food protein-derived renin-inhibitory peptides:in vitroandin vivoproperties

Aluko

2018

J Food Biochem

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Renin catalyzes the rate-determining step in the renin-angiotensin-aldosterone system that regulates mammalian blood pressure by converting angiotensinogen to angiotensin I (Ang I). Excessive plasma levels of Ang I is a causative factor in hypertension development. Therefore, inhibition of renin activity can lower blood pressure and provide relief from clinical symptoms associated with hypertension. Synthetic compounds are currently the most used group of renin inhibitors; however, only aliskiren is approved as a drug for hypertension treatment. But some negative side effects are associated with aliskiren therapy, which have necessitated the search for alternative natural compounds such as food protein-derived renin-inhibitory peptides with blood pressure-reducing effects. This paper is a concise review of the currently known sources and methods of production of renin-inhibitory peptides including their potential in vitro and in vivo extent of renin inhibition. Practical applicationsHypertension is a major human chronic disease that leads to severe cardiovascular impairment and ultimately death if not managed properly. Current therapeutic approach to hypertension management involves the use of drugs that inhibit excessive activities of renin and angiotensin converting enzyme (ACE), the two main enzymes that control mammalian blood pressure. Since renin catalyzes a single reaction that is the rate-determining step in the renin-angiotensin system, inhibition of this enzyme activity could be a highly effective strategy for controlling blood pressure without severe negative side effects. However, therapeutic control of renin activity remains difficult with only one approved drug. Some food protein-derived peptides have been found to inhibit renin activity inhibition, which could offer a drug-free treatment for hypertension. Therefore, this review provides a summary of recent developments in the advances and efficacy testing of renin-inhibitory peptides. K E Y W O R D Sbioactive peptides, blood pressure, food proteins, protein hydrolyzates, renin, renin-angiotensin system, spontaneously hypertensive rats How to cite this article: Aluko RE. Food protein-derived renin-inhibitory peptides: in vitro and in vivo properties.

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Kinetics of in vitro renin and angiotensin converting enzyme inhibition by chicken skin protein hydrolysates and their blood pressure lowering effects in spontaneously hypertensive rats

Cited by 52 publications

References 31 publications

Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates

Structural and Antihypertensive Properties of Enzymatic Hemp Seed Protein Hydrolysates

Efectos fisiológicos de los péptidos bioactivos derivados de las proteínas del lactosuero en la salud: Una revisión

Food protein-derived renin-inhibitory peptides:in vitroandin vivoproperties

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