Kinetics of Lymphocytic Subsets in Chicken Tracheal Lesions Infected with Infectious Bronchitis Virus.

Abstract

ABSTRACT. The kinetics of T-cells (CD3 positive (+), CD4+ and CD8+ cells) and B-cells (IgG+, IgM+ and IgA+ cells) in chicken trachea were studied immunohistochemically and histopathologically following an intratracheal inoculation of infectious bronchitis virus (IBV). Viral antigen was detected in the cytoplasm of tracheal epithelium from 16 hr to 6 days post-inoculation (p.i.) with a peak on 4 days p.i. A few IgG+, IgM+ and IgA+ cells were detected in the submucosa from 8 hr p.i. Thereafter IgG+ and IgM+ cells were gradually increased in number, and dramatically increased from 3 days p.i., peaked on 4 days p.i., and gradually decreased after 5 days p.i. IgA+ cells were detected in a small number than IgG+ and IgM+ cells during the all experimental period. These B cells mainly existed in the lamina propria, and some cells were recognized in the interepithelial space. After 14 days p.i., small number of IgG+ and IgM+ cells were detected in the germinal center of lymph follicles in the lamina propria. From 24 to 60 hr p.i., a few number of CD3+, CD4+ and CD8+ cells were detected at the perivascular area in the lamina propria. After 3 or 4 days p.i., each positive T-cells increased rapidly in number, and reached on the peak at 5 days p.i. CD3+, CD4+ and CD8+ cells tend to distribute diffusely, perivascular area, and surrounding area of CD4+ cells, respectively. CD4+ cells were dramatically decreased from 7 days p.i., and CD3+ and CD8+ cells were decreased from 14 days p.i. No T-cells were detected in the lymph follicles in the lamina propria.-KEY WORDS: B lymphocyte, chicken, infectious bronchitis virus, T lymphocyte, trachea.

Multivalent DNA Vaccine Enhanced Protection Efficacy against Infectious Bronchitis Virus in Chickens

“…The CD8 + CTL have been reported to be critical in the control of infectious bronchitis in poultry [9,27], while the CD4 + T-cell responses may increase the proliferation, maturation, and functional activity of CD8 + CTL, and directly produce the antiviral cytokines [17,19]. Our results support an important role of N protein in protecting against IBV.…”

Proventriculitis in Broiler Chickens: Immunohistochemical Characterization of the Lymphocytes Infiltrating the Proventricular Glands

“…activity, recognition of target antigens in conjunction with the major histocompatibility complex, and production of lymphokines such as interferon-␥, interleukin-2, and tumor necrosis factor-␤. 13,26 Cells mediating these different activities can be identified by cell-surface antigens, CD4ϩ for helper T cells, CD8ϩ for cytotoxic and suppressor T cells, and CD3ϩ as a shared T-cell antigen. The activation of B cells and their differentiation into antibody-secreting plasma cells is triggered by antigen and requires helper T cells.…”

Induction of respiratory immune responses in the chicken; implications for development of mucosal avian influenza virus vaccines

“…However, the tracheal mucosa is highly responsive to infection and reacts with extensive lymphocyte infiltration followed by proliferation (Gaunson et al 2000;Gaunson et al 2006;Matthijs et al 2009). Tracheal lesions characteristic for Mycoplasma infections predominantly consist of proliferating B cells (Gaunson et al 2006) and this is similar to IBV infection (Kotani et al 2000). The cells are mainly located in the lamina propria and submucosa (Kotani et al 2000).…”

“…Tracheal lesions characteristic for Mycoplasma infections predominantly consist of proliferating B cells (Gaunson et al 2006) and this is similar to IBV infection (Kotani et al 2000). The cells are mainly located in the lamina propria and submucosa (Kotani et al 2000). Responses in trachea are agedependent as B cells were not detected in the trachea of Mycoplasma gallisepticum-infected SPF layer-type chicken until 3 weeks post infection (Gaunson et al 2006).…”