Kinetics of molecular transport across a liposome bilayer

Srivastava, Abneesh; Eisenthal, Kenneth B.

doi:10.1016/s0009-2614(98)00662-9

Cited by 150 publications

(217 citation statements)

References 15 publications

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“…Therefore, SHG can be generated from this kind of material provided the size of the microparticle is comparable to the coherence length of the SHG, i.e., roughly the wavelength of the SH light. [22][23][24][25][26][27][28][29][30][31][32][33] One of the promising applications of this method is to investigate transport kinetics of molecules across liposome bilayers. 28,31,33 Liposomes are spherical self-assembled bilayer structures that separate two aqueous phases.…”

Section: Methodsmentioning

confidence: 99%

Effects of Counterions on Molecular Transport Across Liposome Bilayer: Probed by Second Harmonic Generation

et al. 2001

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The transport rate of an organic cation, malachite green (MG), across a unilamellar bilayer (∼105 nm) of the dioleoylphosphatidylglycerol (DOPG) liposome has been studied by the SHG technique. This is the first time to our knowledge that the effects of anions on molecular cation transport have been observed. Our studies show four results. First, in the presence of sodium chloride (NaCl) or sodium bromide (NaBr), the time constant for transport of MG across the DOPG bilayer increases with the increase in the concentration of the counterion (i.e., Cl -or Br -). Second, with the organic electrolytes, sodium citrate (NaCitrate) and sodium ethanesulfonate (NaEtSO 3 ), the transport rate is independent of the concentration of the counterion (i.e., Citrateand EtSO 3 -). Third, at the same counterion concentration, the transport rate depends on the species of the counterion used. The rate of MG transport is the slowest with Cl -, faster with Br -, and the fastest with the two organic counterions, Citrate -and EtSO 3 -. Last, at the low counterion concentration of 1mM, the transport rates of MG were found to approach the same value for the four anions. A brief discussion of a possible mechanism is presented.

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Section: Methodsmentioning

confidence: 99%

Effects of Counterions on Molecular Transport Across Liposome Bilayer: Probed by Second Harmonic Generation

et al. 2001

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“…In addition to probing adsorption and transport across living cell membranes [17,18,20], SHG has been applied to other biologically relevant systems including liposomes [21][22][23][24][25], neurons [26], and collagen networks [27,28].…”

Section: Introductionmentioning

confidence: 99%

Adsorption and transport of charged vs. neutral hydrophobic molecules at the membrane of murine erythroleukemia (MEL) cells

Zeng

Eckenrode

Dai

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…The SHG method, on the other hand, is capable of measuring molecular transport across model lipid bilayers (liposomes) in real time and without the need for direct compound detection [7,8]. According to the conditions of the SHG signal detection, it allows differentiating molecules present in the bulk solution from those molecules in the region of interest, namely the interface of bilayer and water.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

A fluorescence method for determining transport of charged compounds across lipid bilayer

Przybyło¹,

Olżyńska²,

Han³

et al. 2007

Biophysical Chemistry

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT3 AbstractThere is a constant need for simple, economical and time-efficient methods which allow evaluating a compound's ability to penetrate the biological membrane, one of the key parameters needed to characterize biologically active compounds. In the paper we propose a new method of a permeability determination. Instead of detecting the compound's concentration directly, we employ an approach in which the membrane interface is labeled with a fluorescein lipid probe; the probe is sensitive to the presence of charged compounds. The fluorescence intensity changes of the dye permanently attached to both sides of a model lipid bilayer are measured. Specifically, the time course of the fluorescence intensity changes following a rapid induction of a nonequilibrium state of the sample allows the evaluation of the membrane permeability for the compound. The method was validated by the determination of the phenyltin compound's transport through the model phosphatidylcholine unilamellar liposome bilayer.

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Kinetics of molecular transport across a liposome bilayer

Cited by 150 publications

References 15 publications

Effects of Counterions on Molecular Transport Across Liposome Bilayer: Probed by Second Harmonic Generation

Effects of Counterions on Molecular Transport Across Liposome Bilayer: Probed by Second Harmonic Generation

Adsorption and transport of charged vs. neutral hydrophobic molecules at the membrane of murine erythroleukemia (MEL) cells

A fluorescence method for determining transport of charged compounds across lipid bilayer

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