Abstract. In this paper we consider a control problem for an uncertain chemostat model with a general growth function and cell mortality. This uncertainty affects the model (growth function) as well as the outputs (measurements of substrate). Despite this lack of information, an upper bound and a lower bound for those uncertainties are assumed to be known a priori. We build a family of feedback control laws on the dilution rate, giving a guaranteed estimation on the unmeasured variable (biomass), and stabilizing the two variables in a rectangular set, around a reference value of the substrate. We give two realistic applications of this control law to a depollution process and to phytoplankton culture.