Kinetics of protein tyrosine phosphorylation in sperm selected by binding to homologous and heterologous oviductal explants: how specific is the regulation by the oviduct?

Petrunkina, A. M.; Simon, Katrin; Günzel‐Apel, A.‐R.; Töpfer‐Petersen, Edda

doi:10.1016/j.theriogenology.2003.09.011

Cited by 39 publications

(31 citation statements)

References 31 publications

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“…By contrast, the nearest source of porcine oviducts was from an abattoir 130 km away. While the number of canine spermatozoa that bound to canine and porcine oviducts was similar [48], the number of ejaculated boar spermatozoa in our study that bound to the isthmus (but not ampulla) was significantly less in cows than gilts. This result suggests that binding is preferentially species-specific because carbohydrate-binding lectins and glycoconjugates present on the plasma membrane of the sperm head and surface of oviductal epithelium may vary considerably among species [29,50].…”

Section: Discussioncontrasting

confidence: 57%

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Oviduct binding ability of porcine spermatozoa develops in the epididymis and can be advanced by incubation with caudal fluid

et al. 2015

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Abstract.The sperm reservoir is formed when spermatozoa bind to the epithelium of the utero-tubal junction and caudal isthmus of the oviduct. It is an important mechanism that helps synchronize the meeting of gametes by regulating untimely capacitation and polyspermic fertilisation. This study investigated the influence of epididymal maturation and caudal fluid on the ability of spermatozoa to bind to oviduct epithelium using a model porcine oviduct explant assay. Spermatozoa from the rete testis, middle caput (E2-E3), middle corpus (E6) and cauda (E8) of Large White or Large White x Landrace boars at 10-14 months of age were diluted in modified Androhep solution and incubated with porcine oviduct explants. Results reported in this study support our hypothesis that testicular spermatozoa need to pass through the regions of the epididymis in order to acquire the ability to bind to the oviduct. There was a sequential increase in the number of spermatozoa that bound to oviduct explants from the rete testis to caudal epididymis. Binding of caudal spermatozoa to isthmic explants was the highest (15.0 ± 1.2 spermatozoa per 1.25 mm 2 ; mean ± standard error of the mean; P ≤ 0.05) and lowest by spermatozoa from the rete testis (2.0 ± 0.3 per 1.25 mm 2 ), and higher to isthmus from sows compared to gilts (35.8 ± 6.7 per 1.25 mm 2 vs. 14.8 ± 3.0 per 1.25 mm 2 ; P ≤ 0.05). Binding of ejaculated spermatozoa to porcine isthmus was higher than for caudal spermatozoa (26.3 ± 1.4 per 1.25 mm 2 vs. 15.0 ± 0.8 per 1.25 mm 2 ; P ≤ 0.05), and higher to porcine than to bovine isthmus (26.3 ± 2.3 per 1.25 mm 2 vs. 18.8 ± 1.9 per 1.25 mm 2 ; P ≤ 0.05). Incubation of spermatozoa from the caput and corpus in caudal fluid 2 increased the ability of spermatozoa to bind to oviduct epithelium (P ≤ 0.05). In conclusion, the capacity of testicular spermatozoa to bind to oviduct epithelium increases during their maturation in the epididymis, and can be advanced by components of the caudal fluid.

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Section: Discussioncontrasting

confidence: 57%

“…The use of a heterologous system for studying sperm binding to oviductal epithelium has been examined in several species including the binding of human spermatozoa to the oviducts of cows and macaques [47], canine spermatozoa to porcine oviducts [48] and stallion spermatozoa to bovine oviductal cells [49].…”

Section: Discussionmentioning

confidence: 99%

Oviduct binding ability of porcine spermatozoa develops in the epididymis and can be advanced by incubation with caudal fluid

et al. 2015

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“…The lyophilized and conjugated secondary antibody Cy3 was diluted to 1 mg/mL in distilled H 2 O and stored at 4 °C until further use [29,30].…”

Section: Ethidium Homodimer and Tyrosine Phosphorylation (Tp-eh) Staimentioning

confidence: 99%

“…Anti-phospho-tyrosine antibodies can be used to immune-fluorescently detect phosphorylation of tyrosine residues in the tail of capacitated sperm [6,7,[24][25][26][27][28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Validation of merocyanine 540 staining as a technique for assessing capacitation-related membrane destabilization of fresh dog sperm

Steckler¹,

Stout

Durandt

et al. 2015

Theriogenology

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The aim of this study was to determine whether flow cytometric evaluation of combined merocyanine 540 and Yo-Pro 1 staining (M540-YP) would identify viable dog sperm that had undergone membrane stabilization known to be associated with capacitation in other species, and whether such destabilization is detected earlier than when using the tyrosine phosphorylation and ethidium homodimer stain combination (TP-EH) with epifluorescence microscopy. Semen from nine dogs was collected and incubated in parallel in bicarbonate-free modified Tyrode"s medium (-BIC), in medium containing 15 mM bicarbonate (+BIC), in dog prostatic fluid (PF), and in phosphate buffered saline (PBS). Aliquots for staining were removed at various time points during incubation of up to 6 hours. Staining with M540-YP allowed the classification of dog sperm as viable without destabilized membranes, viable with 2 destabilized membranes, non-viable without destabilized membranes or non-viable with destabilized membranes. The percentage of viable sperm detected using EH (83.5 ± 1.37%; mean ± SEM) was higher than when using YP (66.7 ± 1.37%: P < 0.05; n=54 semen samples). On the other hand, M540-YP identified a higher percentage of viable sperm with destabilized membranes than TP-EH (75 ± 1.76% vs. capacitation was recorded during incubation in PBS. We conclude that YP identifies sperm committed to cell death earlier than EH, and that the M540-YP stain combination identifies membrane destabilization known to be associated with capacitation in other species earlier than the TP-EH stain combination.

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Dynamic quantification of the tyrosine phosphorylation of the sperm surface proteins during capacitation

Piehler¹,

Petrunkina

Ekhlasi-Hundrieser³

et al. 2006

Cytometry Pt A

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Background: Spermatozoa acquire active fertilizing competence only after deposition in the female tract and subsequent capacitation. Recent studies on the cellular location of major sperm phosphoproteins suggest that capacitation is associated with tyrosine phosphorylation of proteins exposed on the sperm surface. However, these changes have not yet been quantified objectively. A calcium influx seems to be required for the completion of tyrosine phosphorylation in some species; however, the exact temporal coordination between these processes is still poorly understood. Methods: Flow cytometry was used to quantify the degree of phosphorylation of the sperm surface proteins by probing with fluorescein isothiocyanate-conjugated antiphosphotyrosine (pY) antibody raised in mouse. Dynamic changes in other sperm parameters (calcium influx, membrane integrity, and spontaneous acrosome reaction) were assessed to analyze their temporal coordination. Results: The changes in specific phosphotyrosine (pY) fluorescence signal detected in live, nonpermeabilized boar cell suspensions were biphasic during incubation under capacitating conditions. After 120 min of incubation, the degree of pY fluorescence increased threefold,

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Kinetics of protein tyrosine phosphorylation in sperm selected by binding to homologous and heterologous oviductal explants: how specific is the regulation by the oviduct?

Cited by 39 publications

References 31 publications

Oviduct binding ability of porcine spermatozoa develops in the epididymis and can be advanced by incubation with caudal fluid

Oviduct binding ability of porcine spermatozoa develops in the epididymis and can be advanced by incubation with caudal fluid

Validation of merocyanine 540 staining as a technique for assessing capacitation-related membrane destabilization of fresh dog sperm

Dynamic quantification of the tyrosine phosphorylation of the sperm surface proteins during capacitation

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