Kinetics of uptake of 2‐deoxy‐d‐glucose and 2‐aminoisobutyric acid in chemically transformed cells

Kuroki, Toshio; Yamakawa, Shigeyo

doi:10.1002/ijc.2910130211

Intl Journal of Cancer

1974

DOI: 10.1002/ijc.2910130211

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Kinetics of uptake of 2‐deoxy‐d‐glucose and 2‐aminoisobutyric acid in chemically transformed cells

Toshio Kuroki

Shigeyo Yamakawa

Abstract: Kinetics of 2-deoxy-D-glucose and 2-aminoisobutyric acid uptake in chemically transformed hamster embryo cells and BA LB 3T3 cells were investigated. Maximum velocity of the glucose uptake in chemically transformed cells increased 1.5 to 2.8-fold over controls in Vmax without a detectable change in apparent Km. In uptake of the amino acid there was a slight change in Vmax and unchanged Km with chemically transformed cells, as compared to untransformed cells. Hexokinase activity showed a slight change after che… Show more

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Cited by 9 publications

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“…Furthermore, a direct correlation has been established between the increased sugar transport and the transformed morphology of cells Martin et al, 1971; Stephenson et al, 1973). Fibroblastic cells transformed in vitro by chemical carcinogens also show a higher uptake of 2-deoxyglucose as compared to normal cells (Kuroki and Yamakawa, 1974; Oshiro and DiPaolo, 1974).…”

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Studies on the uptake of 2‐deoxy‐d‐glucose in normal and malignant rat epithelial liver cells in culture

Siddiqi

Iype

1975

Intl Journal of Cancer

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The rate of uptake of 2-deoxyglucose in normal rat hepatic cells and chemically induced hepatoma cells in culture was studied. The hepatoma cells possessed a higher permeability to the sugar at all stages of culture. However, a decrease in the uptake of 2-deoxyglucose at confluency was observed in cells which exhibited density-dependent inhibition of growth, whether normal or malignant. The normal cells in mitosis showed an increased permeability to the sugar, whereas no such change was observed in the hepatoma cells. The kinetic studies of 2-deoxyglucose transport in normal and transformed rat liver showed a positive correlation between the increase in Vmax and the transformed state of the cells, whether they were transformed in vitro or in vivo. No changes in the apparent Km were found, indicating that there are no qualitative changes in the transport sites. The results suggest that an increase in the number of sites involved in glucose transport is a characteristic of chemically transformed rat liver epithelial cells.

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confidence: 96%

Studies on the uptake of 2‐deoxy‐d‐glucose in normal and malignant rat epithelial liver cells in culture

Siddiqi

Iype

1975

Intl Journal of Cancer

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Effects of dibutyryl cyclic adenosine 3′: 5′ monophosphate and theophylline on 2‐deoxy‐D‐glucose and 2‐aminoisobutyric acid uptake by hamster embryo cells

Kuroki¹,

Yamakawa²

1974

Intl Journal of Cancer

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The uptake of 2-deoxy-D-glucose by hamster embryo cells was stimulated by preincubation with I mM dibutyryl cyclic adenosine 3':S monophosphate (dibutyryl CAMP) and theophylline for 24 h. The increase in uptake depended on the concentration of dibutyryl cAMP and was accelerated by u combination o j dibutyryl cAMP and theophylline. The increased uptake of 2-deoxy-D-ghcose was reversed by removal of these chemicals. Lineweaver-Burk plots of the uptake indicated that these chemicals caused an increase in the maximum velocity oj2-deoxy-D-glucose uptake but no detectable change in the apparent Km. Hexokinase was not activated by preincubation of cells with I m M dibutyryl CAMP and theophylline. The uptake of glucose in nine other cell lines, including those from rat and mouw embryos, was not stimulated by these chemicals. In contrast to the uptake of 2-deoxy-D-glucose, that of 2-aminoisobutyric acid was inhibited by these compounds.

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The stimulation of 2‐deoxy‐D‐glucose transport in control and virus‐transformed cells by ethidium bromide

Soslau

Nass

1975

Journal Cellular Physiology

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Recently we demonstrated that ethidium bromide altered the plasma and subcellular membrane glycoproteins in control and virus transformed cells. It is reported here that ethidium bromide also stimulated the membrane associated process of sugar transport. The KM of the virus transformed cells and the ethidium bromide treated cells is the same as that of the control cells while the maximum velocity as compared to the control cells is significantly increased. The transport of 2-deoxyl-D-glucose was inhibited by glucose, cytochalasin B and neuraminidase but was unaffected by variations in cell density or pH of the incubation medium.

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Kinetics of uptake of 2‐deoxy‐d‐glucose and 2‐aminoisobutyric acid in chemically transformed cells

Cited by 9 publications

References 20 publications

Studies on the uptake of 2‐deoxy‐d‐glucose in normal and malignant rat epithelial liver cells in culture

Studies on the uptake of 2‐deoxy‐d‐glucose in normal and malignant rat epithelial liver cells in culture

Effects of dibutyryl cyclic adenosine 3′: 5′ monophosphate and theophylline on 2‐deoxy‐D‐glucose and 2‐aminoisobutyric acid uptake by hamster embryo cells

The stimulation of 2‐deoxy‐D‐glucose transport in control and virus‐transformed cells by ethidium bromide

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