2023
DOI: 10.1101/2023.03.23.534015
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Kinetochore dynein is sufficient to biorient chromosomes and remodel the outer kinetochore

Abstract: Multiple microtubule-directed activities concentrate on chromosomes during mitosis to ensure their accurate distribution to daughter cells. These activities include couplers and dynamics regulators localized at the kinetochore, the specialized microtubule interface built on centromeric chromatin, as well as motor proteins recruited to kinetochores and to mitotic chromatin. Here, we describe an in vivo reconstruction approach in which the effect of removing the major microtubule-directed activities on mitotic c… Show more

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