Kinins and peritoneal exudates induced by carrageenin and zymosan in rats

Abstract

1 Kinins were measured by a radioimmunoassay in the inflammatory exudates induced by carrageenin or zymosan in the peritoneal cavity of normal Wistar rats and of kininogen-deficient Brown Norway rats. 2 After administration of carrageenin to normal rats, levels of immunoreactive kinins showed a single peak during the first two hours and then decreased. The presence of kinins preceded and accompanied the exudation of '25I-labelled albumin. Kinins were identified as bradykinin by chromatography. 2 Captopril, an inhibitor of kininase 2, increased the level of kinins and the volume of the exudates after carrageenin treatment. In Brown Norway rats, the volume of the exudates was small and contained little or undetectable amounts of immunoreactive kinins. 4 During zymosan-induced peritonitis, the exudates were devoid of immunoreactive kinins in both species. The volume of the exudates was larger in kininogen-deficient rats than in normal rats. 5 We conclude that in rats, the kinin system is a major factor responsible for the development of the inflammatory reactions induced by carrageenin, but is not involved in the reactions induced by zymosan.

Comparison with Various Parts ofBroussonetia papyriferaas to the Antinociceptive and Anti-Inflammatory Activities in Rodents

“…The third phase (2.5 h after carrageenan treatment) is related to prostaglandins and leukotriens. [8][9][10][11] Based on the our above results, the antinociceptive and anti-inflammatory mechanisms of BP might be related mainly to kinins and autocrines, because BP radix and fruit mainly inhibited the latephase of the formalin-induced licking response and the first two phase of carrageenan-induced paw edema.…”

Involvement of B1 and B2 receptors in bradykinin‐induced rat paw oedema

“…Kinins are recognized as potent vasoactive peptides which promote venular dilatation, increased vascular permeability, enhanced fluid secretion from epithelia and produced pain and hyperalgesia (Lewis, 1970;Garcia Leme, 1978;Marceau et al, 1983;Proud & Kaplan, 1988;Steranka & Burch, 1991;Dray & Perkins, 1993). In addition, inflammation is associated with increased levels of BK and its metabolites des-Arg9-BK and des-Arg'0-Lys-BK (Regoli & Barabe, 1980;Hargreaves et al 1988;Damas et al, 1990). 'Author for correspondence.…”

Anti-Inflammatory Activity of the Total Flavonoid Fraction from Broussonetia papyrifera in Combination with Lonicera japonica

“…On the other hand, our preparation (EBP and BL) interfered with the pathways of eicosanoid and nitric oxide production. Indeed, 5-LOX products such as leukotrienes and COX products such as PGE2 are major players that lead to the production of edematic response in AA-induced ear edema and 5 h CGN-paw edema models (Vinegar et al, 1969;Holsapple and Yim, 1984;Inoue et al, 1988;Damas et al, 1990). Accordingly, the strong anti-inflammatory activity of EBP and BL in these animal models of inflammation may be associated with their inhibitory effects on 5-LOX, COX and iNOS.…”