Kitasatospora sampliensis sp. nov., a novel actinobacterium isolated from soil of a sugar-cane field in India

Abstract

Polyphasic characterization of an actinomycete strain VT-36 T isolated from a sugar-cane field soil sample collected in Punjab State, India, revealed that the strain belongs to the genus Kitasatospora. The strain's chemotaxonomic characters and G+C content of DNA (76?5 mol%) were typical of members of the genus. Analysis of the 16S rRNA gene sequence supported the generic affiliation of the strain and showed that its closest phylogenetic relative was Kitasatospora putterlickiae F18-98 T (=DSM 44665 T ) (98?3 % 16S rRNA gene sequence similarity). The similarities with type strains of all other Kitasatospora species were in the range 95?1-97?0 %. The results of DNA-DNA hybridization showed 54 % relatedness of the isolate and K. putterlickiae F18-98 T . Based on the above data and the phenotypic differences from K. putterlickiae and other Kitasatospora species, it is proposed that the isolate should be classified as the type strain of a novel species, Kitasatospora sampliensis sp. nov., with strain VT-36 T (=MTCC 6546 T = DSM 44898 T =JCM 13010 T ) as the type strain. Strain VT-36 T was isolated from a sugar-cane field soil sample collected at Sampli village, Punjab state, India, by using Streptomyces agar (1?0 % malt extract, 0?4 % yeast extract, 0?4 % glucose, 0?2 % calcium carbonate and 1?2 % agar) complemented with cycloheximide (25 ml ml 21 ) and amphotericin B (10 ml ml 21 ) at 28 u C. The pure culture was maintained as a glycerol suspension (10 % v/v) of spores and mycelial fragments at 270 uC. The reference strain K. putterlickiae MTCC 6643 T was obtained from the Microbial Type Culture Collection (MTCC), Chandigarh, India.Strain VT-36 T was grown on Streptomyces agar for morphological and physiological characteristics. Morphological properties were examined according to the methods reported by Williams et al. (1983). Growth and sporulation were observed on standard media-like actinomycetes isolation agar (0?2 % sodium caseinate, 0?01 % asparagine, 0?4 % sodium propionate, 0?05 % dipotassium phosphate, 0?01 % magnesium sulphate, 0?0001 % ferrous sulphate and 1?5 % agar, pH

Untitled

“…Actinobacteria represents a large fraction of microbiomes in the root systems and is well established that they are dominant fraction of the microbial community in soils of wild and agricultural plant species [47][48][49][50][51]. Together with other phyla, the members of actinobacteria account for a large proportion in the rhizosphere of numerous plants including wheat (Triticum aestivum) [9,17,44,52,53]; rice (Oryza sativa) [48,54,55]; maize (Zea mays) [50,56,57]; Sugarcane (Saccharum officinarum) [58][59][60]; soybean (Glycine max) [51,[61][62][63]; pea (Pisum sativum) [51,64,65]; sunflower (Helianthus annuus) [49,66,67] and chickpea (Cicer arietinum) [68,69].…”

Kitasatospora cathayae sp. nov., a novel endophytic actinomycete isolated from the leaves of Cathaya argyrophylla

“…The members of the genus Kitasatospora have ability to produce bioactive compounds [3][4][5]. With the gradual exploitation of biodiversity, in order to find more organisms with the strong potential of novel bioactive substances, more attention is being paid to Kitasatospora strains from unusual and unexplored environments, such as rhizosphere soil [6][7][8][9], tubers of yam beam [10], pine grove soil [11], soil humus [12], tropical peat swamp forest soil [13] and sugar-cane field soil [14]. During the process of investigating the diversity of rhizosphere and endophytic actinomycetes from Cathaya argyrophylla, more than 200 strains were isolated by the dilutionplate method on different culture media.…”

Kitasatospora saccharophila sp. nov. and Kitasatospora kazusanensis sp. nov., isolated from soil and transfer of Streptomyces atroaurantiacus to the genus Kitasatospora as Kitasatospora atroaurantiaca comb. nov.

“…55 LI et al was re-established by phylogenetic study based on 16S rRNA gene sequence data (Zhang et al, 1997). The twenty species of Kitasatospora have been validly published at the time of writing with recently described species K. sampliensis (Mayilraj et al, 2006), K. viridis (Liu et al, 2005), and K. putterlickiae, K. arboriphila, K. gansuensis, K. nipponensis, K. paranensis, andK.…”