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Ducos, J; Alenton, R.; Reano, J.F.; Kanchanomai, Chaorai; Deshayes, Alain; Pétiard, Vincent

doi:10.1023/a:1023915613158

Cited by 32 publications

(15 citation statements)

References 18 publications

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“…At every 14-day subculture, 0.5 g FEC displayed a two-to fourfold biomass increase. This fact, also observed by Ducos et al (2003), indicated that the FEC had a high rate of cell division.…”

Section: Cytogenetic Analysissupporting

confidence: 54%

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Cytogenetic and flow cytometry data expand knowledge of genome evolution in three Coffea species

2012

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Karyotype and nuclear 2C-value data are considered important in taxonomic and evolutionary approaches in Coffea. Still, new methods are needed to further support such studies, especially to determine the progenitors of Coffea arabica. In this work, new cytogenetic and flow cytometry data were used to compare Coffea arabica, Coffea canephora and Coffea congensis. These data corroborate the hypothesis that C. canephora and C. congensis originated from a single ancestor, whose basic chromosome number was x = 11. In agreement with the observations of other authors, the karyotype and mean 2C-values confirm that C. arabica is a true allotetraploid originating from two diploid Coffea species with similar genomes. Although C. canephora and C. congensis have been considered potential progenitors of C. arabica, karyotype comparison revealed that only one of these species may be parental to C. arabica. These accurate cytogenetic and flow cytometry data contribute to expand our knowledge of the Coffea genome, as well as of possible progenitors of C. arabica.

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Section: Cytogenetic Analysissupporting

confidence: 54%

“…2). Ducos et al (2003) warned to restrict the embryogenic proliferation period in Coffea to less than 5-7 months. Jain and de Klerk (1998) reported that somaclonal variation frequencies could increase by prolonging the callus proliferation phase.…”

Section: Cytogenetic Analysismentioning

confidence: 99%

Cytogenetic and flow cytometry data expand knowledge of genome evolution in three Coffea species

2012

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“…Ducos et al (2003) made a trial in the field with more than 5,000 plants. The study of morphological characteristics and yield did not produce any significant differences between the plants derived from embryogenic tissues and microcuttings.…”

Section: Scale-upmentioning

confidence: 99%

“…The most productive lines showed a cumulative yield of green coffee of more than 3,000 kg ha −1 . Somaclonal variation at the DNA level or the occurrence of phenotypic variants that could appear later during the development of the plants cannot be excluded (Ducos et al 2003). In our laboratory, we used amplified fragment length polymorphism (AFLP) to evaluate the variability in regenerated plantlets of C. arabica obtained by direct or indirect SE.…”

Section: Scale-upmentioning

confidence: 99%

Somatic Embryogenesis in Coffea spp.

Loyola‐Vargas

Avilez-Montalvo

Aviles-Montalvo

et al. 2016

Somatic Embryogenesis: Fundamental Aspects and Applications

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Coffee is a very important commodity in the world. It is a perennial plant and as a consequence its genetic improvement had been difficult. The biotechnology, in particular somatic embryogenesis (SE) has helped to the propagation of commercial coffee cultivars. Along almost 50 years, different research groups around the world have developed uncountable SE processes to produce an efficient, reproducible, and reliable SE process. Many of the factors that affect the embryogenic response have been studied. It has been demonstrated that the genetic modification of coffee is possible. This transformation can lead to the improvement of varieties with very attractive agronomic traits, such as low caffeine content, resistant to diseases and pests. IntroductionCoffee is one of the two major commodities in the world. The cultivation of coffee gives employment to more than 80 million people, and every year, more than 11 million hectares are cultivated around the world.All coffee species are trees or perennial woody bushes. The coffee plants differ among themselves in different aspects such as morphology, size, and ecological adaptation. Although there are more than 124 species of the Coffea genus, only two are used to produce coffee commercially, Coffea arabica and C. canephora, which accounts for 60 and 40 % of world coffee production, respectively. C. canephora (2n = 2x = 22) is an outcrossing diploid, allogamous, consisting of polymorphic populations of strongly heterozygous individuals and with high caffeine content. C. arabica is an allotetraploid (2n = 4x = 44) derived from a spontaneous

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“…It therefore depends on both genetic and environmental (culture conditions) factors. Conformity studies, conducted in the field on robusta clones have shown that SE techniques result in plants that are true to type (Ducos et al 2003a). Recently, the use of SE has been applied for large scale production of C. canephora elite clones (Ducos et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and vegetative cutting capacity are under distinct genetic control in Coffea canephora Pierre

et al. 2010

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The purpose of the study was to evaluate the possible genetic effect on vegetative propagation of Coffea canephora. Diversity for somatic embryogenesis (SE) ability was observed not only among two groups of C. canephora Pierre (Congolese and Guinean), but also within these different genetic groups. The results therefore showed that, under given experimental conditions, SE ability is depending on genotype. Furthermore the detection of quantitative trait loci (QTLs) controlling the SE and cutting abilities of C. canephora was performed on a large number of clones including accessions from a core collection, three parental clones and their segregating progenies. On the one hand we detected eight QTLs determining SE. Six positive QTLs for SE ability, whatever the criteria used to quantify this ability, were localized on one single chromosome region of the consensus genetic map. Two negative QTLs for SE ability (frequency of micro calli without somatic embryo) were detected on another linkage group. Deep analysis of the six QTLs detected for SE ability came to the conclusion that they can be assimilated to one single QTL explaining 8.6–12.2% of the observed variation. On the other hand, two QTLs for average length of roots and length of the longest sprouts of cuttings were detected in two linkage groups. These QTLs detected for cutting ability are explaining 12–27% of the observed variation. These observations led to conclude that SE and cutting abilities of C. canephora Pierre appeared to be genetic dependent but through independent mechanisms.

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Cited by 32 publications

References 18 publications

Cytogenetic and flow cytometry data expand knowledge of genome evolution in three Coffea species

Cytogenetic and flow cytometry data expand knowledge of genome evolution in three Coffea species

Somatic Embryogenesis in Coffea spp.

Somatic embryogenesis and vegetative cutting capacity are under distinct genetic control in Coffea canephora Pierre

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