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Cerović, Zoran G.; Langrand, Emmanuelle; Latouche, Gwendal; Morales, Fermı́n; Moya, I.

doi:10.1023/a:1006081209887

Cited by 21 publications

(5 citation statements)

References 42 publications

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“…Endogenous fluorescence was evaluated in two spectral regions: the green (peaking between 540 and 560 nm) and the red (peaking at 680 nm). Previously, the NADPH dependent blue-green fluorescence (BGF) was described in isolated chloroplasts [16,17]. In our experiments, green/yellow fluorescence stimulated by excitation at 450 nm was most pronounced between 510 and 560 nm and this fluorescence inside the cells is most likely derived from NADPH-related flavins and flavonoids.…”

Section: Discussionsupporting

confidence: 58%

“…Chlorophyll a is a well-established fluorescing pigment employed in photosynthesis [15]. In addition to the chlorophyll fluorescence, NADPH dependent blue-green fluorescence (BGF) was also described in isolated chloroplasts [16,17], while most of the green fluorescence in the intercellular space was associated with lignins of the intermediate lamella [18]. We previously demonstrated a sensitivity of endogenous fluorescence to various environmental stressors employing the spectrally and time-resolved microscopy in algae [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Biosensing the Presence of Metal Nanoparticles by Spectrally- and Time-Resolved Endogenous Fluorescence in Water Moss Fontinalis antipyretica

2021

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Pollution by heavy metals represents a significant environmental burden. We employed confocal microscopy with spectral detection and fluorescence lifetime imaging (FLIM) to evaluate the effect of nanoparticles (NPs) from various metals (Zinc, Nickel, Cobalt, Copper) on endogenous fluorescence of Fontinalis antipyretica moss. Short term (3–5 day) exposure to NPs, designed and fabricated by direct synthesis using femtosecond laser ablation in water, was studied. The green flavonoid and/or lignin endogenous fluorescence peaking between 500 and 560 nm was found to be increased by Zn and significantly reduced by Cu. The overall red chlorophyll fluorescence intensity with a maximum of 680 nm remained largely unchanged after exposure to Ni and Zn, but was decreased in the presence of Co and completely abolished by Cu. All NPs but Zn induced changes in the fluorescence lifetimes, demonstrating increased sensitivity of this parameter to environmental pollution. Gathered data indicate fast responsiveness of the endogenous fluorescence in the Fontinalis antipyretica moss to the presence of heavy metals that can thus potentially serve as a biosensing tool for monitoring environmental pollution in the moss natural environment.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Biosensing the Presence of Metal Nanoparticles by Spectrally- and Time-Resolved Endogenous Fluorescence in Water Moss Fontinalis antipyretica

2021

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“…In addition to the chlorophyll fluorescence, we observed also the presence of much lower, but sustained green/yellow fluorescence. Previously, the blue-green fluorescence (BGF) was described in isolated chloroplasts, where it showed a reversible increase of BGF when illuminated with light, and it has been proposed that this light-induced variation of chloroplast BGF is completely NADPH dependent [48,49]. Energy transfer from proteins to NADPH in chloroplasts was proposed to affect fluorescence lifetimes [50,51].…”

Section: Discussionmentioning

confidence: 99%

Time-resolved endogenous chlorophyll fluorescence sensitivity to pH: study on Chlorella sp. algae

Chorvatova

Uherek

Mateášik

et al. 2020

Methods Appl. Fluoresc.

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To better understand pH-dependence of endogenous fluorescence of algae, we employed spectroscopy and microscopy methods, including advanced time-resolved fluorescence imaging microscopy (FLIM), using green algae Chlorella sp. as a model system. Absorption spectra confirmed two peaks, at 400–420 nm and 670 nm. Emission was maximal at 680 nm, with smaller peaks between 520 and 540 nm. Acidification led to a gradual decrease in the red fluorescence intensity with the maximum at 680 nm when excited by 450 nm laser. FLIM measurements, performed using 475 nm picoseconds excitation, uncovered that this effect is accompanied by a shortening of the tau1 fluorescence lifetime. Under severe acidification, we also noted an increase in the green fluorescence with a maximum between 520–540 nm and a shift toward 690–700 nm of the red fluorescence, accompanied by prolongation of the tau2 fluorescence lifetime. Gathered data increase our knowledge on the responsiveness of algae to acidification and indicate that endogenous fluorescence derived from chlorophylls can potentially serve as a biosensing tool for monitoring pH change in its natural environment.

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“…All these fluorescence emission bands were also identified by Atta et al [17] while studying the bacterial leaf blight (BLB) disease in rice; Atta et al designated the molecular assignments to these bands. The bands at 500-600 nm represent several phenolic compounds including the cinnamic acids [18], nicotinamide adenine dinucleotide phosphate [28], alkaloids (berberine and quercetin) [29] and hydrocarbon carotenes (violaxanthin and antheraxanthin) and β-carotene [15]. The prominent emission bands at 680 and 730 nm wavelengths represent chlorophyll a [15,18,29,30].…”

Section: Fluorescence Spectroscopy Of Grapefruitmentioning

confidence: 99%

Synchronous fluorescence spectroscopy for early diagnosis of citrus canker in citrus species

Atta¹,

Saleem²,

Ali³

et al. 2019

Laser Phys.

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Citrus canker is among the most destructive diseases of citrus crops that cause huge economic losses owing to a reduction in citrus production and at the same time threaten the marketability of citrus [1,2]. This disease is prevalent in most citrus-growing countries around the globe [3]. Citrus canker is caused by the bacterium Xanthomonas axonopodis pv. citri (Syn. X. citri pv. citri). However, the bacteria associated with the disease have been divided into different pathotypes based on a host range and symptomology. These include pathotypes; A (X. axonopodis pv. citri), B and C (X. axonopodis pv. aurantifolii), and E (X. axonopodis pv. citrumelo) [4]. Citrus canker symptoms on leaves, stems and fruits consist of Laser Physics

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Untitled

Cited by 21 publications

References 42 publications

Biosensing the Presence of Metal Nanoparticles by Spectrally- and Time-Resolved Endogenous Fluorescence in Water Moss Fontinalis antipyretica

Biosensing the Presence of Metal Nanoparticles by Spectrally- and Time-Resolved Endogenous Fluorescence in Water Moss Fontinalis antipyretica

Time-resolved endogenous chlorophyll fluorescence sensitivity to pH: study on Chlorella sp. algae

Synchronous fluorescence spectroscopy for early diagnosis of citrus canker in citrus species

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