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Bigham, Shelli R.; Coffer, Jeffery L.

doi:10.1023/a:1009049823345

Cited by 18 publications

(11 citation statements)

References 35 publications

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“…[53][54][55][56][57] The photoluminescence of semiconductor quantum dots was used to probe the effect of DNA structure on its binding to the nanoparticles. [53][54][55]58] It was found that "kinked" oligonucleotides, which exhibit a curvature of % 3 nm from crystallographic and biochemical experiments, bind more tightly and faster to NPs that display curvatures of similar dimensions relative to straight or bent DNA.…”

Section: Functionalization Of Nanoparticles With Biomolecules Throughmentioning

confidence: 99%

Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications

2004

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Nanomaterials, such as metal or semiconductor nanoparticles and nanorods, exhibit similar dimensions to those of biomolecules, such as proteins (enzymes, antigens, antibodies) or DNA. The integration of nanoparticles, which exhibit unique electronic, photonic, and catalytic properties, with biomaterials, which display unique recognition, catalytic, and inhibition properties, yields novel hybrid nanobiomaterials of synergetic properties and functions. This review describes recent advances in the synthesis of biomolecule-nanoparticle/nanorod hybrid systems and the application of such assemblies in the generation of 2D and 3D ordered structures in solutions and on surfaces. Particular emphasis is directed to the use of biomolecule-nanoparticle (metallic or semiconductive) assemblies for bioanalytical applications and for the fabrication of bioelectronic devices.

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Section: Functionalization Of Nanoparticles With Biomolecules Throughmentioning

confidence: 99%

Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications

2004

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“…Semiconductor nanocrystals composed of a variety of materials can be directly grown in aqueous solutions, such as CdS [63,[102][103][104][105][106][107][108][109] and CdTe [63,110,111]. However, the size distribution and quantum yield of these particles in general is worse than that of particles grown in organic surfactants like TOPO.…”

Section: Water-soluble Colloidal Nanocrystalsmentioning

confidence: 99%

Biological applications of colloidal nanocrystals

et al. 2003

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Due to their interesting properties, research on colloidal nanocrystals has moved in the last few years from fundamental research to first applications in materials science and life sciences. In this review some recent biological applications of colloidal nanocrystals are discussed, without going into biological or chemical details. First, the properties of colloidal nanocrystals and how they can be synthesized are described. Second, the conjugation of nanocrystals with biological molecules is discussed. And third, three different biological applications are introduced: (i) the arrangement of nanocrystal-oligonucleotide conjugates using molecular scaffolds such as single-stranded DNA, (ii) the use of nanocrystal-protein conjugates as fluorescent probes for cellular imaging, and (iii) a motility assay based on the uptake of nanocrystals by living cells.

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“…[11] The only previous report of DNA-templated growth on PbS has yielded materials with no detectable luminescence in the infrared. [12] We worked instead at a synthesis temperature at which chemical interaction was possible between the metal cations used in PbS growth and at least two classes of sites on DNA: the phosphate backbone, and also DNA's purine and pyrimidine bases.…”

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confidence: 99%

Efficient Infrared‐Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood Plasma

et al. 2005

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Quantum-dot nanocrystals have been used to label single molecules during living-cell assays [1] and provide direct visual guidance and real-time confirmation of complete resection during cancer surgery in an animal model. [2] The use of quantum dots for deep-tissue imaging accompanied by low autofluorescence in vivo requires emission in the second infrared biological window of 1000±1200 nm combined with stability in biological media. Surface chemistry determines the chemical and optical stability of quantum dots. A stabilizing outer shell minimizes diffusion of oxygen to the surface of the core of the nanoparticle, as demonstrated using a high-bandgap semiconductor shell [3] and a dielectric shell.[4] Silanized nanoparticles have been shown to be water soluble and to retain the absorption and emission spectra of the original particles; however, the nanoparticles lost 60±80 % of their original quantum efficiency in this process. Recently, oligomeric phosphines [5] have been employed to form three thin concentric sublayers around quantum dots: an inner phosphine layer for dot-surface passivation, a linking layer for protection, and an outer functionalized layer for miscibility and subsequent chemical modification or conjugation to biomolecules. In the infrared, the application of this multistep synthetic method to type-II core±shell nanoparticles has resulted in quantum dots that show modest degradation in 37 C plasma over the course of half an hour.[3]Here we adopt an entirely different strategy: we report the first growth of efficient infrared photoluminescent quantum dots directly on a DNA template. Our infrared-emitting quantum dots grown on the biomolecular template are efficient and stable in water, serum, and blood plasma.DNA has previously been decorated with metal nanoparticles 5±10 nm in diameter through the use of thiol linkages. [6] DNA has also been used as a long-term stabilizer and template in the growth of CdS nanocrystals, but with no reports of a photoluminescence quantum efficiency.[7±10] Related progress has also been made in synthesizing CdS nanoparticles in which growth was carried out at room temperature followed by annealing at 80 C to improve photoluminescent properties; quantum efficiencies of 10 ±4 were estimated.[11] The only previous report of DNA-templated growth on PbS has yielded materials with no detectable luminescence in the infrared.[12]We worked instead at a synthesis temperature at which chemical interaction was possible between the metal cations used in PbS growth and at least two classes of sites on DNA: the phosphate backbone, and also DNA's purine and pyrimidine bases. The bases provide an additional opportunity for control over the growth of nanoparticles and the passivation of their surface states. The synthesis reported herein is simple, reproducible, and yields PbS nanoparticles with exceptional stability and photoluminescence quantum efficiency. Energyfiltered transmission electron microscopy (EFTEM) reveals cubic-latticed PbS quantum dots 4 nm in diameter on a network...

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Cited by 18 publications

References 35 publications

Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications

Integrated Nanoparticle–Biomolecule Hybrid Systems: Synthesis, Properties, and Applications

Biological applications of colloidal nanocrystals

Efficient Infrared‐Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood Plasma

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