Klebsiella oxytoca and Emerging Nosocomial Infections

Nakul, Neog; Phukan, Upasana; Puzari, Minakshi; Sharma, Mohan; Chetia, Pankaj

doi:10.1007/s00284-021-02402-2

Cited by 60 publications

(45 citation statements)

References 92 publications

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“…In this scenario, the carbapenemase-producing (CP) K. oxytoca isolates are also emerging as potential life-threatening pathogens. In the nosocomial setting, sporadic CP K. oxytoca can be encountered and two outbreaks due to the KPC or OXA-48 producers have also been described (5)(6)(7). In contrast, only one mcr-1-possessing K. oxytoca strain has been isolated so far from humans (8).…”

Section: Introductionmentioning

confidence: 99%

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Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla _VIM-1 and mcr-9

Campos-Madueno

Moser

Risch

et al. 2021

Antimicrob Agents Chemother

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The spread of plasmid-mediated carbapenemases within Klebsiella oxytoca is well-documented. In contrast, data concerning the closely-related species Klebsiella grimontii are scarce. In fact, despite the recent report of the first bla KPC-2 -producing K. grimontii , nothing is known about its clonality and antibiotic resistance patterns. In a retrospective search in our collection, we identified 2 bla VIM -positive K. oxytoca strains. Whole-genome sequencing with both Illumina and Nanopore indicated that our strains actually belonged to K. grimontii and were of sequence type (ST) 172 and ST189. Moreover, the two strains were associated with 297kb IncHI2/HI2A-pST1 and 90.6kb IncFII(Yp) plasmids carrying bla VIM-1 together with mcr-9 and bla VIM-1 , respectively. In the IncHI2/HI2A plasmid, bla VIM-1 was located in a class 1 integron (In 110 ), while mcr-9 was associated to the qseC - qseB- like regulatory elements. Overall, this plasmid showed to be very similar to those carried by other Enterobacterales isolated from food and animal sources (e.g., Salmonella and Enterobacter spp. detected in Germany and Egypt). The IncFII(Yp) plasmid was unique and its bla VIM-1 region was associated to a rare integron (In 1373 ). Mapping of In 1373 indicated a possible origin in Austria from an Enterobacter hormaechei carrying a highly similar plasmid. Core-genome phylogenies indicated that the ST172 K. grimontii belonged to a clone of identical Swedish and Swiss strains (≤15 SNVs to each other), whereas the ST189 strain was sporadic. Surveillance of carbapenemase-producing K. oxytoca strains should be reinforced to detect and prevent the dissemination of new species belonging to the Klebsiella genus.

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Section: Introductionmentioning

confidence: 99%

“…5 McFarland, and both microbroth (cation-adjusted Mueller Hinton Broth II) and disk-diffusion (Mueller-Hinton agar) susceptibility tests were performed in parallel and interpreted as described above. The mgrB gene was analyzed by that were designed with Geneious Prime (v2021.1.1).…”

mentioning

confidence: 99%

Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla _VIM-1 and mcr-9

Campos-Madueno

Moser

Risch

et al. 2021

Antimicrob Agents Chemother

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“…The gram-negative bacterium Klebsiella , which exists in the normal flora of the oral cavity, skin, and intestine, is an opportunistic pathogen that can lead to nosocomial infection ( Podschun and Ullmann, 1998 ). K. oxytoca is the second most prevalent Klebsiella in the clinic after K. pneumoniae ( Neog et al, 2021 ). Opportunistic K. oxytoca in hospitals mainly infects infants, the elderly, and patients with low immune function, and is the main cause of colitis, endocarditis, sepsis, and urinary and respiratory tract infections ( Beaugerie et al, 2003 ; Ménard et al, 2010 ; Egger et al, 2017 ; Tsubouchi et al, 2019 ; Soto-Hernández et al, 2020 ; Surani et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia

Zhang

Yan

et al. 2021

Front. Microbiol.

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Klebsiella oxytoca is an important nosocomial and community-acquired opportunistic pathogenic Klebsiella and has become the second most prevalent strain in the clinic after K. pneumoniae. However, there have been few reports of bacteriophages used for treating K. oxytoca. In this study, a novel bacteriophage, vB_Kox_ZX8, which specifically infects K. oxytoca AD3, was isolated for the first time from human fecal samples. The biological characteristics of vB_Kox_ZX8 showed an incubation period of 10 min, a burst size of 74 PFU/cell, and a stable pH range of 3–11. Genomic bioinformatics studies of vB_Kox_ZX8 showed that it belongs to the genus Przondovirus, subfamily Studiervirinae, family Autographiviridae. The genome of vB_Kox_ZX8 is 39,398 bp in length and contains 46 putative open reading frames encoding functional proteins, such as DNA degradation, packaging, structural, lysin-holin, and hypothetical proteins. We further investigated the efficacy of vB_Kox_ZX8 phage in the treatment of mice with bacteremia caused by K. oxytoca infection. The results showed that vB_Kox_ZX8 (5 × 109 PFU/mouse) injected intraperitoneally alone was metabolized rapidly in BALB/c mice, and no significant side effects were observed in the control and treatment groups. Importantly, intraperitoneal injection with a single dose of phage vB_Kox_ZX8 (5 × 107 PFU/mouse) for 1 h post-infection saved 100% of BALB/c mice from bacteremia induced by intraperitoneal challenge with a minimum lethal dose of K. oxytoca AD3. However, all negative control mice injected with PBS alone died. Owing to its good safety, narrow host infectivity, high lysis efficiency in vitro, and good in vivo therapeutic effect, phage vB_Kox_ZX8 has the potential to be an excellent antibacterial agent for clinical K. oxytoca-caused infections.

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“…Klebsiella oxytoca is an opportunistic pathogen of antibioticassociated hemorrhagic colitis (33); hence, we investigated the impact of these strains on the colon (Supplementary Figure 12). The results of gene expression and H&E staining showed that these strains did not have an additional burden on the colon.…”

Section: Effects Of Strains On Alcohol-induced Liver Injury Lipid Acc...mentioning

confidence: 99%

Intestinal Microbiota Contributes to the Improvement of Alcoholic Hepatitis in Mice Treated With Schisandra chinensis Extract

et al. 2022

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Alcoholic hepatitis (AH) has a high short-term mortality rate. Schisandra chinensis has the potential to ameliorate liver damage and be a source of prebiotics. We aimed to investigate whether Schisandra chinensis extract (SCE) can improve AH and the role of the small intestinal and cecal microbiota and their metabolites. UHPLC-QE-MS was used to analyze the chemical components of SCE. The chronic-plus-binge ethanol feeding model was used to induce AH in mice. 1H NMR was used to analyze intestinal metabolites. 16S rRNA-based high throughput sequencing was used to evaluate the effects of SCE on intestinal microbiota (IM). Intestinal microbiota transplantation was used to explore the role of IM in SCE treatment of AH. SCE ameliorated AH non-dose-dependently. SCE effectively improved liver inflammation and oxidative/nitrosative stress, strengthened intestinal barrier function, and regulated the composition of IM and the content of short-chain fatty acids (SCFAs) in AH mice. Samples from in vivo and in vitro SCE-altered IM improved liver status and regulated the IM. The administration of Lactobacillus plantarum and Bifidobacterium breve ameliorated AH to some extent. The administration of Enterococcus faecalis and Klebsiella oxytoca had partial beneficial effects on AH. Collectively, IM and metabolites were closely associated with the improvement of SCE on AH. The possible microbe targets were the growth inhibition of Escherichia-Shigella and the expansion of SCFA producers, such as Lactobacillus and Bifidobacterium. Schisandra chinensis can be considered as a safe and effective dietary supplement for the prevention and improvement of AH.

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Klebsiella oxytoca and Emerging Nosocomial Infections

Cited by 60 publications

References 92 publications

Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla _VIM-1 and mcr-9

Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla _VIM-1 and mcr-9

Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia

Intestinal Microbiota Contributes to the Improvement of Alcoholic Hepatitis in Mice Treated With Schisandra chinensis Extract

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Klebsiella oxytoca and Emerging Nosocomial Infections

Cited by 60 publications

References 92 publications

Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla VIM-1 and mcr-9

Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla VIM-1 and mcr-9

Characteristics of a Bacteriophage, vB_Kox_ZX8, Isolated From Clinical Klebsiella oxytoca and Its Therapeutic Effect on Mice Bacteremia

Intestinal Microbiota Contributes to the Improvement of Alcoholic Hepatitis in Mice Treated With Schisandra chinensis Extract

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Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla _VIM-1 and mcr-9

Exploring the Global Spread of Klebsiella grimontii Isolates Possessing bla _VIM-1 and mcr-9