Klebsiella pneumoniae harbouring OXA-48 carbapenemase in a Libyan refugee in Italy

Kocsis, Erika; Savio, C.; Piccoli, M.; Cornaglia, Giuseppe; Mazzariol, Annarita

doi:10.1111/1469-0691.12232

Cited by 31 publications

(21 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, carbapenemase genes different from bla KPC have so far been considered less of a problem, whose magnitude has perhaps not been fully assessed, with only sporadic reports being published (Gaibani et al, 2013;Kocsis et al, 2013). As an example, although VIM carbapenemase was firstly recognized in Italy (Lauretti et al, 1999), the exact magnitude of its distribution in our country is still difficult to establish.…”

Section: Discussionmentioning

confidence: 98%

Evaluation of carbapenem-resistant Enterobacteriaceae in an Italian setting: Report from the trench

Barbarini

Russello

Brovarone

et al. 2015

Infection, Genetics and Evolution

View full text Add to dashboard Cite

The spread of carbapenem resistant Enterobacteriaceae (CRE) has recently become a matter of concern in public health, mainly due to the wide distribution of carbapenemase genes. Italy is a country considered endemic for the spread of blaKPC Klebsiella pneumoniae (KP). The aim of this study was to depict the epidemiological trend of CRE in one Italian hospital over a long period (3 years surveillance, from May 2011 to April 2014). Based on defined MIC cut-off for specific carbapenems, 164 strains isolated from 146 different patients were analyzed both phenotypically and genotypically to establish the resistance genes. Molecular typing was performed using the RAPD technique. 77 strains were demonstrated to harbor the blaKPC gene (73 KP, 4 Escherichia coli - EC), 51 strains the blaVIM gene (44 KP, 3 EC, 2 Enterobacter cloacae and 2 Klebsiella oxytoca), 8 the blaNDM gene (3 KP, 4 EC and one Providencia stuartii), 3 the blaOXA-48 gene (2 KP, 1 EC), whereas 25 out of the 164 isolates (of different genera and species) had a negative multiplex-PCR amplification for all the targets tested. 39 out of the 164 strains analyzed (23.8%) revealed discrepancies between the MICs obtained with automated instrument and gradient MICs of more than two logs of difference; the broth microdilution provided a better agreement with the results obtained with the gradient MIC. The use of RAPD allowed to distinguish different clusters, closely related, both for blaKPC and for blaVIM KP.

show abstract

Section: Discussionmentioning

confidence: 98%

Evaluation of carbapenem-resistant Enterobacteriaceae in an Italian setting: Report from the trench

Barbarini

Russello

Brovarone

et al. 2015

Infection, Genetics and Evolution

View full text Add to dashboard Cite

show abstract

“…3). In Africa, they have been mainly identified in the northern countries (Morocco, Algeria, Tunisia, Egypt, and Libya) [101,[115][116][117][118][119][120][121][122][123][124][125][126], but OXA-48 producers have also been identified in Senegal and South Africa [127,128] (Fig. 3).…”

Section: Thementioning

confidence: 99%

The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide

Nordmann

Poirel

2014

Clinical Microbiology and Infection

620

497

View full text Add to dashboard Cite

The spread of carbapenemase producers in Enterobacteriaceae has now been identified worldwide. Three main carbapenemases have been reported; they belong to three classes of β-lactamases, which are KPC, NDM, and OXA-48. The main reservoirs of KPC are Klebsiella pneumoniae in the USA, Israel, Greece, and Italy, those of NDM are K. pneumoniae and Escherichia coli in the Indian subcontinent, and those of OXA-48 are K. pneumoniae and Escherichia coli in North Africa and Turkey. KPC producers have been mostly identified among nosocomial isolates, whereas NDM and OXA-48 producers are both nosocomial and community-acquired pathogens. Control of their spread is still possible in hospital settings, and relies on the use of rapid diagnostic techniques and the strict implemention of hygiene measures.

show abstract

“…However, three studies reported the presence of OXA-48-producing K. pneumoniae isolated from Libyan patients transferred to Europe for treatment. Indeed, Kocsis et al reported that a carbapenem-resistant K. pneumoniae carrying the blaOXA-48 gene was recovered from the blood culture of a Libyan patient hospitalized in the ICU of the Sacro Cuore-Don Calabria Hospital in Negrar, Italy [38]. Italy is the country with the highest incidence of OXA-48-producing K. pneumoniae isolates, and has been considered to be the epicenter of the spread of this enzyme in Mediterranean basin [39].…”

Section: Indeed Mamlouk Et Al Detected the Blactx-m-15 Gene Inmentioning

confidence: 99%

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Mathlouthi

Al-Bayssari

Salabi

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: The aim of the study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among clinical isolates of Enterobacteriaceae recovered from Tunisian and Libyan hospitals. Methodology: Bacterial isolates were recovered from patients in intensive care units and identified by biochemical tests and MALDI-TOF. Antibiotic susceptibility testing was performed by disk diffusion and the E-test method. ESBL and carbapenemase activities were detected using standard microbiological tests. Antibiotic resistance-encoding genes were screened by PCR and sequencing. Clonal relationships between Klebsiella pneumoniae strains were carried out using multi-locus sequence typing (MLST). Results: A total of 87 isolates were characterized, with 51 and 36, respectively, identified as E. coli and K. pneumoniae. Overall the resistance prevalence was high for aminoglycosides (> 60%), fluoroquinolones (> 80%), and extended-spectrum cephalosporins (> 94%), and was low for imipenem (11.4%). Among this collection, 58 strains (66.6%) were ESBL producers and 10 K. pneumoniae strains (11.4%) were carbapenemase producers. The antibiotic resistance-encoding genes detected were blaCTX-M-15 (51.7%), blaTEM-1 (35.6%), several variants of blaSHV (21.8%), and blaOXA-48 (11.4%). The MLST typing of K. pneumoniae isolates revealed the presence of multiple clones and three novel sequence types. Also, close relationships between the OXA-48-producing strains from Tunisia and Libya were demonstrated. Conclusions: This study is the first paper describing the emergence of carbapenemase-and ESBL-producing Enterobacteriaceae, sensitive to colistin, isolated in Tunisia and Libya. Active surveillance and testing for susceptibility to colistin should be implementing because resistance to colistin, mainly in Klebsiella, has been recently reported worldwide.

show abstract

Klebsiella pneumoniae harbouring OXA-48 carbapenemase in a Libyan refugee in Italy

Cited by 31 publications

References 16 publications

Evaluation of carbapenem-resistant Enterobacteriaceae in an Italian setting: Report from the trench

Evaluation of carbapenem-resistant Enterobacteriaceae in an Italian setting: Report from the trench

The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Contact Info

Product

Resources

About