2008
DOI: 10.1016/j.bbamcr.2008.03.018
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Knockdown of cytosolic 5′-nucleotidase II (cN-II) reveals that its activity is essential for survival in astrocytoma cells

Abstract: IMP preferring cytosolic 5'-nucleotidase (cN-II) is an ubiquitous nucleotide hydrolysing enzyme. The enzyme is widely distributed and its amino acid sequence is highly conserved among vertebrates. Fluctuations of cN-II activity have been associated with the pathogenesis of neurological disorders. The enzyme appears to be involved in the regulation of the intracellular availability of the purine precursor IMP and also of GMP and AMP, but the contribution of this activity and of its regulation to cell metabolism… Show more

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Cited by 41 publications
(40 citation statements)
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“…1), is likely the result of these and other still unknown regulatory mechanisms. In contrast to SAMHD1, the 5′-nucleotidases contributing to the balance of dNTP pools are constitutively expressed proteins whose experimental down-regulation does not induce significant alterations of dNTP pool sizes (33,42). SAMHD1 is clearly a major regulator of dNTP pool turnover, whose loss has large effects on the composition of the cellular dNTP pool both during quiescence and proliferation.…”
Section: Discussionmentioning
confidence: 99%
“…1), is likely the result of these and other still unknown regulatory mechanisms. In contrast to SAMHD1, the 5′-nucleotidases contributing to the balance of dNTP pools are constitutively expressed proteins whose experimental down-regulation does not induce significant alterations of dNTP pool sizes (33,42). SAMHD1 is clearly a major regulator of dNTP pool turnover, whose loss has large effects on the composition of the cellular dNTP pool both during quiescence and proliferation.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, a knockdown study in human astrocytoma cells revealed that cN-II activity was essential for their survival. 19 Thus, additional studies are necessary to identify the underlying molecular mechanism involved in the potential association of purine dysmetabolism with SCZ risk.…”
Section: Tablementioning
confidence: 99%
“…Inhibitors of e5'NT, such as APCP, 3,3 ,4 ,5,7-pentahydroxyflavon (quercetin), 1-amino-4-[4-fluoro-2-carboxyphenylamino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (PSB-0952), sodium nitroprusside (SNP), and 1-amino-4-[2-anthracenylamino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (PSB-0963) as well as anti-CD73 antibodies may potentially treat several diseases in the CNS [62,124,144,254,280,[363][364][365][366][367][368]. Indeed, APCP and quercetin reduced the proliferation of human glioma cells (U138MG cell line) by inhibition of e5'NT activity and, as a consequence, decreased the extracellular levels of the glioma cell proliferation/adhesion stimulator Ado [62,367,369,370].…”
Section: Modulation Of 5'-nucleotidase Activity: a New Promising Thermentioning
confidence: 99%
“…The activity of cN-II may be enhanced by (deoxy)ATP, GTP and ADP [82,[139][140][141]. Cytosolic 5'-nucleotidase II has a phosphotransferase activity from 6-hydroxypurine monophosphates (phosphate donors) to phosphate acceptors, such as Guo, (deoxy)Ino and nucleoside analogs, to form monophosphate derivatives [85,133,[140][141][142][143], and plays a role in (i) purine nucleotide interconversion, (ii) the maintenance/modulation of intracellular 5-phosphoribosyl-1-pyrophosphate (PRPP) and purine nucleotide pools and (iii) the survival of cultured astrocytoma cells [81,133,144].…”
Section: General Properties and Functions Of 5'ntsmentioning
confidence: 99%