Knockdown of eIF4E suppresses cell proliferation, invasion and enhances cisplatin cytotoxicity in human ovarian cancer cells

Wan, Jing; Shi, Fang; Xu, Zhanzhan; Zhao, Min

doi:10.3892/ijo.2015.3201

Cited by 23 publications

(14 citation statements)

References 38 publications

(41 reference statements)

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“…It also has been demonstrated that a subset of mRNAs encoding cancer-associated proteins, such as c-MYC and cyclin D1, were identified to be sensitive to the activity of the eIF4F complex (15) and may serve as a convergence point for hyperactive signaling pathways to promote tumorigenesis (16). The present study confirmed that knockdown of eIF4E in GBC cells inhibited cell proliferation, oncogenic potential and cell cycle arrest, which is consistent with the previously described data (17,18).…”

Section: Discussionsupporting

confidence: 93%

Upregulation of eukaryotic translation initiation factor 4E associates with a poor prognosis in gallbladder cancer and promotes cell proliferation in vitro and in vivo

et al. 2019

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Eukaryotic translation initiation factor 4 (eIF4E) has been demonstrated to promote tumorigenesis in different types of cancer; however, whether eIF4E is involved in the development of GBC is unclear. The present study aimed to explore the biological function of eIF4E in gallbladder cancer (GBC) and identified that the expression level of eIF4E was significantly increased in GBC tissues compared with that in normal gallbladder tissues. The overall survival (OS) was also shorter in the group of patients with GBC with increased eIF4E expression. Increased eIF4E was correlated with advanced stage and higher histologic grade. Knockdown of eIF4E significantly inhibited cell proliferation, colony formation and cell cycle-associated protein expression levels in 2 GBC cell lines. The weight of the tumors in the eIF4E knockdown group was remarkably decreased compared with the control group. It also was revealed that knockdown of eIF4E is associated with upregulating cyclin-dependent kinase inhibitor 1B and down-regulating the expression levels of cyclin E1 and cyclin D1 in vitro and in vivo . These data demonstrated that eIF4E is a novel prognostic marker in GBC and may serve a critical role in the regulation of cell proliferation.

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Section: Discussionsupporting

confidence: 93%

Upregulation of eukaryotic translation initiation factor 4E associates with a poor prognosis in gallbladder cancer and promotes cell proliferation in vitro and in vivo

et al. 2019

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“…GO analysis revealed that the OCC-1-repressed genes are mainly enriched for the regulators of gene expression that function at post-transcriptional and translational level ( 25 ). Meanwhile, many of these OCC-1-repressed genes have been found to directly involve in cancer cell growth, such as HNRNPA1 ( 47 ) and HNRNPK ( 48 ), which encode splicing factors, EIF3M ( 49 ) and EIF4E ( 50 ), which encode eukaryotic translation initiation factors, and mitotic division-associated genes MAD2L1 ( 51 ) and NEK2 ( 52 ). We reasoned that the upregulation of these genes is directly responsible for the increased cell growth in OCC-1 knockdown cells.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer

Yang

Xiao

et al. 2018

Nucleic Acids Research

162

146

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Overexpressed in colon carcinoma-1 (OCC-1) is one of the earliest annotated long noncoding RNAs (lncRNAs) in colorectal cancer (CRC); however, its function remains largely unknown. Here, we revealed that OCC-1 plays a tumor suppressive role in CRC. OCC-1 knockdown by RNA interference promotes cell growth both in vitro and in vivo, which is largely due to its ability to inhibit G0 to G1 and G1 to S phase cell cycle transitions. In addition, overexpression of OCC-1 can suppress cell growth in OCC-1 knockdown cells. OCC-1 exerts its function by binding to and destabilizing HuR (ELAVL1), a cancer-associated RNA binding protein (RBP) which can bind to and stabilize thousands of mRNAs. OCC-1 enhances the binding of ubiquitin E3 ligase β-TrCP1 to HuR and renders HuR susceptible to ubiquitination and degradation, thereby reducing the levels of HuR and its target mRNAs, including the mRNAs directly associated with cancer cell growth. These findings reveal that lncRNA OCC-1 can regulate the levels of a large number of mRNAs at post-transcriptional level through modulating RBP HuR stability.

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“…According to Choi et al, eIF4e-positivity was associated with advanced tumour stage (III and IV) and reduced patient prognosis [21]. Other gynaecological malignancies, including cervical cancer [22][23][24][25], ovarian carcinoma [19,[26][27][28][29] and breast cancer [30][31][32], have been investigated more thoroughly regarding the prognostic, predictive and therapeutic relevance of eIFs. In cervical cancer, overexpression of eIF5a2 as detected by immunohistochemistry correlates with poor patient prognosis [25].…”

Section: Discussionmentioning

confidence: 99%

The Prognostic Significance of Eukaryotic Translation Initiation Factors (eIFs) in Endometrial Cancer

Smolle

Czapiewski

Łapińska‐Szumczyk

et al. 2019

IJMS

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Whilst the role of eukaryotic translation initiation factors (eIFs) has already been investigated in several human cancers, their role in endometrial cancer (EC) is relatively unknown. In the present retrospective study, 279 patients with EC (1180 samples) were included (mean age: 63.0 years, mean follow-up: 6.1 years). Samples were analysed for expression of 7 eIFs subunits (eIF2α, eIF3c, eIF3h, eIF4e, eIF4g, eIF5, eIF6) through immunohistochemistry and western blotting. Fifteen samples of healthy endometrium served as controls. Density and intensity were assessed and mean combined scores (CS) calculated for each patient. Upon immunohistochemistry, median eIF5 CS were significantly higher in EC as compared with non-neoplastic tissue (NNT, p < 0.001), whilst median eIF6 CS were significantly lower in EC (p < 0.001). Moreover, eIF5 (p = 0.002), eIF6 (p = 0.032) and eIF4g CS (p = 0.014) were significantly different when comparing NNT with EC grading types. Median eIF4g CS was higher in type II EC (p = 0.034). Upon western blot analysis, eIF4g (p < 0.001), peIF2α (p < 0.001) and eIF3h (p < 0.05) were significantly overexpressed in EC, while expression of eIF3c was significantly reduced in EC as compared with NNT (p < 0.001). The remaining eIFs were non-significant. Besides tumour stage (p < 0.001) and patient’s age (p < 0.001), high eIF4g CS-levels were independently associated with poor prognosis (HR: 1.604, 95%CI: 1.037–2.483, p = 0.034). The other eIFs had no prognostic significance. Notably, the independent prognostic significance of eIF4g was lost when adding tumour type. Considering the difficulties in differentiating EC type I and II, eIF4g may serve as a novel prognostic marker indicating patient outcome.

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Knockdown of eIF4E suppresses cell proliferation, invasion and enhances cisplatin cytotoxicity in human ovarian cancer cells

Cited by 23 publications

References 38 publications

Upregulation of eukaryotic translation initiation factor 4E associates with a poor prognosis in gallbladder cancer and promotes cell proliferation in vitro and in vivo

Upregulation of eukaryotic translation initiation factor 4E associates with a poor prognosis in gallbladder cancer and promotes cell proliferation in vitro and in vivo

Long noncoding RNA OCC-1 suppresses cell growth through destabilizing HuR protein in colorectal cancer

The Prognostic Significance of Eukaryotic Translation Initiation Factors (eIFs) in Endometrial Cancer

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