2021
DOI: 10.3389/fagro.2021.715823
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Knockdown of Genes Involved in Transcription and Splicing Reveals Novel RNAi Targets for Pest Control

Abstract: RNA interference (RNAi) is a promising next generation technology for the development of species-specific pest management. The key to successful RNAi based-plant protection is dependent in part on data-driven target gene selection, a challenging task due to the absence of laboratory strains and the seasonality of most pest species. In this study, we aimed to identify novel target genes by performing a knowledge-based approach in order to expand the spectrum of known potent RNAi targets. Recently, the protein-c… Show more

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Cited by 5 publications
(3 citation statements)
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References 89 publications
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“…Counterintuitively, the proteasome is known to be a stable complex, at least in the rat liver, with a half-life of up to 15 days (Tanaka & Ichihara, 1989;Pack et al, 2014;Marshall & Vierstra, 2019). This is in contrast to the general assumption that the protein of a good RNAi target should be unstable for it to be sufficiently susceptible to mRNA depletion (Knorr et al, 2021). Hence, other factors such as mRNA turnover and the proteasome's vital function might be making the proteasome a very effective RNAi target.…”
Section: The Proteasome Is An Excellent Target For Rnai-based Pest Co...mentioning
confidence: 97%
“…Counterintuitively, the proteasome is known to be a stable complex, at least in the rat liver, with a half-life of up to 15 days (Tanaka & Ichihara, 1989;Pack et al, 2014;Marshall & Vierstra, 2019). This is in contrast to the general assumption that the protein of a good RNAi target should be unstable for it to be sufficiently susceptible to mRNA depletion (Knorr et al, 2021). Hence, other factors such as mRNA turnover and the proteasome's vital function might be making the proteasome a very effective RNAi target.…”
Section: The Proteasome Is An Excellent Target For Rnai-based Pest Co...mentioning
confidence: 97%
“…The potential of RNAi as a control strategy against insect pests was first shown in beetles [ 21 ] and moths [ 22 ]. Prominent targets include nucampholin (NCM), Ras opposite (ROP), RNA polymerase II subunit-140 (RPII-140), and dre4, which were effective for the control of the red flour beetle Tribolium castaneum [ 23 , 24 ]. In Aedes albopictus , potential target genes for RNAi-based control in mosquitoes include chitin synthase, β-tubulin, and the inhibitor of apoptosis (see [ 25 ] for a review).…”
Section: Introductionmentioning
confidence: 99%
“…Since these two landmark studies, researchers have continued to demonstrate potential applications for RNAi technology in crop protection, through targeted downregulation of genes in other agricultural pest insects. While some studies have examined the use of species as screening models for identifying sensitive RNAi targets across crop pest taxa (Knorr et al, 2018(Knorr et al, , 2021Mehlhorn et al, 2021), sensitivity of a target gene in one species may not necessarily apply to another species, given the physiological and ecological differences between species, especially between distantly related taxa.…”
Section: Introductionmentioning
confidence: 99%