Knockdown of RAI14 suppresses the progression of gastric cancer

Chen, Cheng; Aihemaiti, Maimaiti; Zhang, Xin; Qü, Hui; Sun, Qi-Long; He, Qingsi; Yu, Wenbin

doi:10.2147/ott.s175502

Cited by 22 publications

(21 citation statements)

References 27 publications

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“…Recently, high expression of RAI14 in gastric cancer tissues, compared to matched normal tissues, was related to unfavourable patients' prognosis [251]. Moreover, RAI14 knockdown inhibited proliferation, migration, and invasion, and promoted apoptosis, through the down-regulation of Akt pathway and its downstream Cyclin D1, where the downstream target gene was RAB31 [252].…”

Section: Retinoid-inducible Gene 1 (Rig1) Hypermethylationmentioning

confidence: 99%

Retinoic Acids in the Treatment of Most Lethal Solid Cancers

Costantini

Molinari

Farinon

et al. 2020

JCM

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Although the use of oral administration of pharmacological all-trans retinoic acid (ATRA) concentration in acute promyelocytic leukaemia (APL) patients was approved for over 20 years and used as standard therapy still to date, the same use in solid cancers is still controversial. In the present review the literature about the top five lethal solid cancers (lung, stomach, liver, breast, and colon cancer), as defined by The Global Cancer Observatory of World Health Organization, and retinoic acids (ATRA, 9-cis retinoic acid, and 13-cis retinoic acid, RA) was compared. The action of retinoic acids in inhibiting the cell proliferation was found in several cell pathways and compartments: from membrane and cytoplasmic signaling, to metabolic enzymes, to gene expression. However, in parallel in the most aggressive phenotypes several escape routes have evolved conferring retinoic acids-resistance. The comparison between different solid cancer types pointed out that for some cancer types several information are still lacking. Moreover, even though some pathways and escape routes are the same between the cancer types, sometimes they can differently respond to retinoic acid therapy, so that generalization cannot be made. Further studies on molecular pathways are needed to perform combinatorial trials that allow overcoming retinoic acids resistance.

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Section: Retinoid-inducible Gene 1 (Rig1) Hypermethylationmentioning

confidence: 99%

Retinoic Acids in the Treatment of Most Lethal Solid Cancers

Costantini

Molinari

Farinon

et al. 2020

JCM

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“…RAI14 promotes mTOR-mediated inflammation under inflammatory stress and chemical hypoxia in U87 glioblastoma cell line [33]. Knockdown of RAI14 suppresses the progression of gastric cancer [34]. High expression of RAI14 in gastric cancer is revealed and such expression pattern could be an independent molecular predictor of poor prognosis in gastric cancer patients [35].…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p/RAI14 axis

Liu

Pei

et al. 2020

BMC Cancer

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Background: Melanoma is the most aggressive skin cancer that derived from pigment cells, accounting for the majority of the skin-cancer-related deaths. Despite great development and evolution have been made in surgery, radiotherapy and adjuvant chemotherapy, the prognosis of melanoma patients exhibited no significant improvement. Long noncoding RNAs (lncRNAs) are frequently dysregulated and involved in the development of cancers. LncRNA AFAP1-AS1 has been explored in various cancers, whereas its role and regulatory mechanism in melanoma are not well understood. Methods: The expression of AFAP1-AS1 was detected by qRT-PCR. CCK-8, colony formation, transwell and western blot assays were performed to investigate the biological role of AFAP1-AS1 in melanoma. Male BALB/c nude mice were applied for in vivo experiments. The interaction among AFAP1-AS1, miR-653-5p and RAI14 was investigated by RNA pull down, RIP and luciferase reporter assays. Results: AFAP1-AS1 was highly expressed in melanoma cell lines. Suppression of AFAP1-AS1 impaired cell proliferation, migration, invasion and EMT in melanoma. Moreover, AFAP1-AS1 was a ceRNA of RAI14 by competitively binding with miR-653-5p. Besides, miR-653-5p overexpression or RAI14 inhibition could repress tumor growth. Eventually, rescue assays indicated that the function of AFAP1-AS1 in the cellular process of melanoma was dependent on miR-653-5p and RAI14. Conclusions: AFAP1-AS1 exerts its oncogenic function in melanoma by targeting miR-653-5p/RAI14 axis.

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“…Retinoic acid induced 14 (RAI14), also known as NORPEG, is an actin-binding protein initially observed to be a regulatory protein at the ectoplasmic specialization. Recent studies highlight that RAI14 was up-regulated in gastric cancer associated with the patient's prognosis and RAI14 knockdown by siRNA interference reduced proliferation and migration, promoted apoptosis through inhibiting the activation of Akt signaling pathway in gastric cancer [34,35]. Wang et al [36] proved that NRP1 was a hypomethylated-upregulated gene in GC patients, which was significantly correlated with tumor malignant phenotypes.…”

Section: Discussionmentioning

confidence: 99%

A methylation‐based mRNA signature predicts survival in patients with gastric cancer

Sun

Zhang

et al. 2020

Cancer Cell Int

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Background: Evidence suggests that altered DNA methylation plays a causative role in the occurrence, progression and prognosis of gastric cancer (GC). Thus, methylated-differentially expressed genes (MDEGs) could potentially serve as biomarkers and therapeutic targets in GC. Methods: Four genomics profiling datasets were used to identify MDEGs. Gene Ontology enrichment and Kyoto Encyclopaedia of Genes and Genomes pathway enrichment analysis were used to explore the biological roles of MDEGs in GC. Univariate Cox and LASSO analysis were used to identify survival-related MDEGs and to construct a MDEGs-based signature. The prognostic performance was evaluated in two independent cohorts. Results: We identified a total of 255 MDEGs, including 192 hypermethylation-low expression and 63 Hypomethylation-high expression genes. The univariate Cox regression analysis showed that 83 MDEGs were associated with overall survival. Further we constructed an eight-MDEGs signature that was independent predictive of prognosis in the training cohort. By applying the eight-MDEGs signature, patients in the training cohort could be categorized into high-risk or low-risk subgroup with significantly different overall survival (HR = 2.62, 95% CI 1.71-4.02, P < 0.0001). The prognostic value of the eight-MDEGs signature was confirmed in another independent GEO cohort (HR = 1.35, 95% CI 1.03-1.78, P = 0.0302) and TCGA-GC cohort (HR = 1.85, 95% CI 1.16-2.94, P = 0.0084). Multivariate cox regression analysis proved the eight-MDEGs signature was an independent prognostic factor for GC. Conclusion: We have thus established an innovative eight-MDEGs signature that is predictive of overall survival and could be a potentially useful guide for personalized treatment of GC patients.

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Knockdown of RAI14 suppresses the progression of gastric cancer

Cited by 22 publications

References 27 publications

Retinoic Acids in the Treatment of Most Lethal Solid Cancers

Retinoic Acids in the Treatment of Most Lethal Solid Cancers

Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p/RAI14 axis

A methylation‐based mRNA signature predicts survival in patients with gastric cancer

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