Knockdown of SAR1B suppresses proliferation and induces apoptosis of RKO colorectal cancer cells

Abstract

Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide. SAR1 gene homolog B (SAR1B) is a GTPase that has been reported to have a central role in the regulation of lipid homeostasis and is associated with numerous diseases. However, its role in cancer, particularly in CRC, remains unclear. The present study revealed that SAR1B was overexpressed in CRC samples and this was associated with shorter overall survival time in patients with CRC. Colony formation, cell proliferation and flow cytometry assays were conducted to evaluate the functions of SAR1B in CRC. It was reported that SAR1B may be associated with tumorigenesis of CRC. Knockdown of SAR1B suppressed cell proliferation and induced significant apoptosis of RKO cells. Furthermore, microarray analysis was performed to identify the potential targets of SAR1B in CRC. Bioinformatics analysis revealed that SAR1B was significantly involved in regulating 'TGF-β signaling', 'paxillin signaling', 'cell cycle regulation by BTG family proteins' and 'IGF-1 signaling'. These results suggested that SAR1B may be considered a potential prognostic biomarker and therapeutic target for CRC.

Sar1 Interacts with Sec23/Sec24 and Sec13/Sec31 Complexes: Insight into Its Involvement in the Assembly of Coat Protein Complex II in the Microsporidian Nosema bombycis

“…After the knockdown of NbSar1 , we found that the proliferation of N. bombycis was significantly inhibited ( Fig. 8 ), which is consistent with previous research showing that the knockdown of Sar1B suppressed cell proliferation and induced significant apoptosis of RKO (colorectal cancer) cells ( 45 ). In Colaphellus bowringi , the knockdown of each gene of COPII considerably inhibited yolk deposition and ovarian growth.…”

SAR1B contributes to progression of lung cancer via enhancing cell proliferation and migration

“…However, the function of SAR1B in tumors has not been fully explored and was only reported recently to have a correlation with the progression of colorectal cancer. More speci cally, SAR1B knockdown has been shown to hinder the proliferation of colorectal cancer cells in vitro and trigger apoptosis [12,13]. In an agreement, we found that SA1R1B was also essential for in vitro cell proliferation capacity in lung cancer.…”

“…Lipid metabolism is an important cellular life process, and abnormalities in its metabolism are also closely related to the development of tumors [11]. Recently, SAR1B is reported to promote proliferation of colorectal cancer cells and induces apoptosis [12], and plays important role in colorectal cancer metastasis [13]. Importantly, Chen et al revealed that SAR1A promoted an mTORC1-dependent growth of lung cancer cells [14].…”

Using period analysis to timely assess and predict 5‐year relative survival for colorectal cancer patients in Taizhou, eastern China

“… 16 Recently, it has been reported that some biomarkers are closely related to the prognosis of CRC. High expressions of SAR1B (secretion associated Ras related GTPase 1B), 17 HOXA11‐AS (homeobox A11 antisense RNA), 18 or TRIM24 (tripartite motif containing 24) 19 in CRC samples had the correlation with poor prognosis and shorter overall survival. Meanwhile, low expression of ENO2 and ENO3 were correlated with longer overall survival.…”