Knocking down a putativeΔ¹-pyrroline-5-carboxylate dehydrogenasegene by RNA interference inhibits flight and causes adult lethality in the Colorado potato beetleLeptinotarsa decemlineata(Say)

Abstract

It seems that identified Ldp5cdh encodes a functional P5CDh enzyme, and Ldp5cdh may serve as a potential target for dsRNA-based pesticide for controlling the damage and dispersal of L. decemlineata adults. © 2014 Society of Chemical Industry.

RNAi‐mediated plant protection against aphids

“…2,7,8 RNA interference (RNAi) refers to double-stranded RNA (dsRNA)-mediated gene silencing, which occurs widely among plants, animals and microorganisms. 9,10 Since its first discovery in Caenorhabditis elegans, 11 targeted gene knockdown through dsRNA-mediated RNAi has been widely used for genetic research in several insect orders, including Diptera, 12 Coleoptera, 13,14 Acari, 15 Orthoptera, 16 Blattodea, 17 Lepidoptera, 18,19 Isoptera 20 and hemipteran pests such as planthopper 21,22 psyllid, 23,24 whitefly 25,26 and aphid. 27,28 Similarly, transgenic plants expressing dsRNA designed against insect target genes gain protection against insect herbivory, and this is considered to be the next-generation pest management strategy, with benefits over chemical-based pesticides or traditional biological control because the sequence-specific nature of RNAi allows for individual species and potentially specific orders of pests to be selectively targeted.…”

Double‐stranded RNA delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the Asian citrus psyllid, Diaphorina citri

“…RNA interference (RNAi) refers to double-stranded RNA (dsRNA)-mediated target-gene silencing. 11,12 Since its first discovery in Caenorhabditis elegans, 13 gene knockdown through dsRNA-mediated RNAi has been widely applied for genetic research that includes the economically important insect orders Lepidoptera, 14,15 Coleoptera 16,17 and hemipteran pests such as aphids, 12,18,19 whitefly 20,21 and psyllids. 22,23 Owing to its high sequence-dependent specificity and wide range of gene targets for exploitation, RNAi-mediated insect control is considered as the next-generation pest management strategy with benefits over chemical-based pesticides or traditional biological control.…”

Amplification and quantification of cold‐associated microRNAs in the Colorado potato beetle (Leptinotarsa decemlineata) agricultural pest

“…A better understanding of the molecular and biochemical changes underlying cold adaptation in Colorado potato beetles might reveal novel insights for the development of innovative pest management strategies against this insect. Studies have highlighted the potential of RNA interference (RNAi)-based approaches involving dsRNAs that target essential genes in various insect models including the Colorado potato beetle (Wan et al, 2015;Zhang et al, 2015).…”