Introduction: Organ transplantation has been found to be the only possible treatment in most of the chronic illnesses. It would be possible to save thousands of lives by organ donation. It has been found that the issue of organ donation involves a lot of medical, legal, ethical, organizational and social factors. Although the shortage of organs has been found to be a global problem, the situation in Asia is much worse. The main reasons behind the low percentage of organ donation in India are lack of awareness, improper infrastructure facilities, myths and misconceptions regarding organ donation. Objective: To assess the knowledge and attitude of the people seeking health care, regarding organ donation in rural Kerala. Materials and Methods: A questionnaire was distributed amongst all willing patients and their relatives attending Medical College Hospital, Urban Health training centre and Rural Health training centre, after obtaining informed consent. A sample size of 360 subjects fulfilling inclusion and exclusion criteria was selected by simple random sampling. Results: The mean age of the study population was 53.6±11.8 with slight female preponderance. Majority of the participants had knowledge about donation of eye (97%) and kidney (91%).81% of participants had obtained knowledge regarding organ donation from television and social media. 64.2% had good attitude and thought that organ donation should be promoted. Conclusion: Although most of the participants had knowledge about organ donation, willingness to donate was found to be limited. Healthcare professionals need to take an active role in clarifying any myths and doubts of people regarding organ donation, which cannot be accomplished through electronic media alone.