Knowledge-driven approaches for engineering complex metabolic pathways in plants

Farré, Gemma; Twyman, Richard M.; Christou, Paul; Capell, Teresa; Zhu, Changfu

doi:10.1016/j.copbio.2014.11.004

Cited by 45 publications

(17 citation statements)

References 58 publications

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“…The impact of metabolic engineering and molecular farming in the pharmaceutical industry is going to increase with the introduction of innovative methods throughout the development chain, from discovery [53] through to the introduction of precise genome modifications and targeted interventions using the latest genome editing tools based on the CRISPR/Cas9 system [54], the integration of quality in the design process to develop optimized production processes that can be scaled up to process scale without loss of yield or quality, and the implementation of the latest regulatory guidelines to ensure that processes are robust, reproducible, safe and efficient to encourage regulatory approval. This study showed that BY-2 cells can be cultivated in disposable bioreactors that are more versatile than conventional bioreactors, and cell growth and the yield of a recombinant antibody (20 mg/l) were comparable to standard shake flask cultivation despite the 200-fold increase in scale.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

High-value products from plants: the challenges of process optimization

Fischer¹,

Vasil'ev²,

Twyman³

et al. 2015

Current Opinion in Biotechnology

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Section: Conclusion and Future Prospectsmentioning

confidence: 99%

High-value products from plants: the challenges of process optimization

Fischer¹,

Vasil'ev²,

Twyman³

et al. 2015

Current Opinion in Biotechnology

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“…A number of recent reviews have focused on the engineering of complex metabolic pathways in plant hosts. 13,14 In this section, we focus on strategies and Benjamin J. Kotopka obtained a Bachelor's degree in molecular biology from Princeton University in 2013. He is currently a PhD student in Prof. Christina Smolke's group at Stanford University.…”

Section: Heterologous Phytochemical Production In Plant Systemsmentioning

confidence: 99%

Synthetic biology strategies toward heterologous phytochemical production

Kotopka

Smolke

2018

Nat. Prod. Rep.

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Covering: 2006 to 2018 Phytochemicals are important sources for the discovery and development of agricultural and pharmaceutical compounds, such as pesticides and medicines. However, these compounds are typically present in low abundance in nature, and the biosynthetic pathways for most phytochemicals are not fully elucidated. Heterologous production of phytochemicals in plant, bacterial, and yeast hosts has been pursued as a potential approach to address sourcing issues associated with many valuable phytochemicals, and more recently has been utilized as a tool to aid in the elucidation of plant biosynthetic pathways. Due to the structural complexity of certain phytochemicals and the associated biosynthetic pathways, reconstitution of plant pathways in heterologous hosts can encounter numerous challenges. Synthetic biology approaches have been developed to address these challenges in areas such as precise control over heterologous gene expression, improving functional expression of heterologous enzymes, and modifying central metabolism to increase the supply of precursor compounds into the pathway. These strategies have been applied to advance plant pathway reconstitution and phytochemical production in a wide variety of heterologous hosts. Here, we review synthetic biology strategies that have been recently applied to advance complex phytochemical production in heterologous hosts.

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“…Although here we focus primarily on the engineering of plant parts drawn from signalling and cell biology, there is significant research activity in synthetic metabolic engineering 6,7 , with many recent headlines stemming from successful porting of opiate 8 and cannabinoid 9 production into yeast.…”

Section: Identification and Remediation Of Toxinsmentioning

confidence: 99%

Plant synthetic biology for molecular engineering of signalling and development

Nemhauser

Torii

2016

Nature Plants

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Molecular genetic studies of model plants in the past few decades have identified many key genes and pathways controlling development, metabolism and environmental responses. Recent technological and informatics advances have led to unprecedented volumes of data that may uncover underlying principles of plants as biological systems. The newly emerged discipline of synthetic biology and related molecular engineering approaches is built on this strong foundation. Today, plant regulatory pathways can be reconstituted in heterologous organisms to identify and manipulate parameters influencing signalling outputs. Moreover, regulatory circuits that include receptors, ligands, signal transduction components, epigenetic machinery and molecular motors can be engineered and introduced into plants to create novel traits in a predictive manner. Here, we provide a brief history of plant synthetic biology and significant recent examples of this approach, focusing on how knowledge generated by the reference plant Arabidopsis thaliana has contributed to the rapid rise of this new discipline, and discuss potential future directions.

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Knowledge-driven approaches for engineering complex metabolic pathways in plants

Cited by 45 publications

References 58 publications

High-value products from plants: the challenges of process optimization

High-value products from plants: the challenges of process optimization

Synthetic biology strategies toward heterologous phytochemical production

Plant synthetic biology for molecular engineering of signalling and development

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