Knowledge, perceptions and practices regarding brucellosis in pastoral communities of Kagera Region in Tanzania

Ntirandekura, Jean‐Bosco; Matemba, Lucas E.; Ngowi, Helena; Kimera, Sharadhuli I.; Karimuribo, Esron D.

doi:10.5455/javar.2018.e285

Cited by 15 publications

(29 citation statements)

References 36 publications

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“…We detected Brucella species in cow milk, which is similar to previous reports in Egypt (Wareth, Melzer, Elschner, Neubauer, & Roesler, 2014), in Uganda (Hoffman et al., 2016) and in Tanzania (Assenga et al., 2015; Mathew et al., 2015). This calls for public health attention since some residents in the study area drink unboiled milk as it was reported previously (Ntirandekura, Matemba, Ngowi, Kimera, & Karimuribo, 2018a). We are also reporting the presence of Brucella in goat milk.…”

Section: Discussionmentioning

confidence: 54%

“…The failure to detect Brucella species in aborted materials could have been associated to the transport medium used (liquid nitrogen), long time for conservation of samples before analysis (9 months) and the low DNA concentration among other factors. However, other factors associated with reproductive failures need to be ruled out for a causal relationship between brucellosis and abortions to hold (Ntirandekura, Matemba, Ngowi, et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of Brucella species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania

Ntirandekura

Makene

Kasanga

et al. 2020

Veterinary Medicine & Sci

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Brucellosis is a zoonotic disease of importance to both public health and the livestock industry. The disease is likely to be endemic in Tanzania and little is reported on molecular characterization of Brucella species in pastoral settings. This study aimed at characterizing Brucella species (targeting genus Brucella) infecting humans, cattle and goat in Kagera region (Ngara and Karagwe districts) using real‐time PCR, PCR amplification of 16S rRNA genes and Sanger sequencing. Brucella spp. were detected in 47 samples (19 sera and 28 milk) out of 125 samples (77 sera, 35 milk and 13 aborted materials) using real‐time PCR. All aborted materials (13 samples) were negative to real‐time PCR. Out of the 47 real‐time PCR positive samples (28 milk and 19 sera), 20 samples (10 milk and 10 sera) showed an expected 16S rRNA gene PCR product. Sequence analysis and blasting confirmed the presence of Brucella spp. in pastoral areas of Kagera region. The Brucella spp. from Kagera were phylogenetically grouped in two clades and three branches all closer to B. melitensis, B. abortus and B. suis from USA, Sudan and Iran. However, they were distinct from other species isolated also in USA, New Zealand, Germany and Egypt. This was expected based on the distance between the geographical regions from which the data (nucleotides sequences from 16S gene sequencing) for the phylogeny reconstruction were obtained. This is the first study to report Brucella species identified using 16S rRNA gene sequencing in East and Central Africa. A livestock vaccination program re‐inforced with a high index of Brucella diagnosis is needed to eradicate brucellosis in animals and minimize suffering from Brucella infections in humans in Tanzania.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Brucella species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania

Ntirandekura

Makene

Kasanga

et al. 2020

Veterinary Medicine & Sci

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“…This emergency pig sell contributes to the spread of the virus in resource-poor settings, including between countries (50-52). However, considering the proximity of the Rutana region to Ruvubu National Park, where warthogs are present (20), and the reported uncontrolled movement of wild animal species between Ruvubu National Park in Burundi, Akagera National Park in Rwanda, and the Kimisi and Burigi game reserves in Tanzania (49), the virus spillover from the sylvatic to the domestic cycle cannot be excluded based on the results of this study.…”

Section: Discussionmentioning

confidence: 90%

“…Kagera region on the Tanzanian side and the Rutana region in Burundi share borders, and uncontrolled animal movement, including that of domestic pigs, are more likely to happen between these two regions. For instance, movement of refugees together with their livestock, reported in the area (49), can contribute to the spread of animal diseases, including ASF. It has been reported that in order to reduce the economic loss due to ASF outbreaks, some farmers sell their pigs before they show clinical signs as soon as ASF is suspected.…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of African Swine Fever Virus From the 2018 Outbreak in South-Eastern Burundi

et al. 2020

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African swine fever (ASF) is a contagious viral disease that causes high mortality, approaching 100%, in domestic pigs and wild boars. The disease has neither a cure nor a vaccine, and it is caused by an ASF virus (ASFV), the only member of the family Asfarviridae, genus Asfivirus, and the only known DNA arbovirus. Twenty-four genotypes of ASFV have been described to date, and all of them have been described in Africa. ASF is endemic in Burundi, and several outbreaks have been reported in the country; the disease continues to economically impact on small-scale farmers. This study aimed at genetic characterization of ASFV that caused an ASF outbreak in the Rutana region, Burundi, in the year 2018. Tissue samples from domestic pigs that died as a result of a severe hemorrhagic disease were collected in order to confirm the disease using polymerase chain reaction (PCR) and to conduct partial genome sequencing. Nucleotide sequences were obtained for the B646L (p72) gene, the intergenic fragment between the I73R and I329L genes, and the central variable region (CVR) of the B602L gene. Phylogenetic analysis of the Burundian 2018 ASFV grouped the virus within B646L (p72) genotype X and clustered together with those reported during the 1984 and 1990 outbreaks in Burundi with high nucleotide identity to some ASFV strains previously reported in neighboring East African countries, indicating a regional distribution of this ASFV genotype. Analysis of the intergenic fragment between I73R and I329L genes showed that the Burundian 2018 ASFV described in this study lacked a 32-base pair (bp) fragment present in the reference genotype X strain, Kenya 1950. In addition, the strain described in this study had the signature AAABNAABA at the CVR (B602L) gene and showed 100% amino acid sequence identity to viruses responsible for recent ASF outbreaks in the region. The virus described in this study showed high genetic similarities with ASFV strains previously described in domestic pigs, wild suids, and soft ticks in East African countries, indicating a possible common wild source and continuous circulation in domestic pigs in the region.

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“…Despite the emergence of a signi cant number of new antibiotics, the treatment of intracellular pathogens still represents a challenge in pharmacy [6]. Nowadays, with the preparation of pharmaceutical nanoparticles, unique properties can be achieved that will increase the performance and variety of its drug forms.…”

Section: Serum Level Of Vitamin D Crp and Biochemical Parameter In Amentioning

confidence: 99%

Serum level of vitamin D, CRP and biochemical parameter in acute and chronic brucellosis treated with doxycycline-loaded solid lipid nanoparticles

et al. 2020

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Knowledge, perceptions and practices regarding brucellosis in pastoral communities of Kagera Region in Tanzania

Cited by 15 publications

References 36 publications

Molecular characterization of Brucella species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania

Molecular characterization of Brucella species detected in humans and domestic ruminants of pastoral areas in Kagera ecosystem, Tanzania

Genetic Analysis of African Swine Fever Virus From the 2018 Outbreak in South-Eastern Burundi

Serum level of vitamin D, CRP and biochemical parameter in acute and chronic brucellosis treated with doxycycline-loaded solid lipid nanoparticles

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