Serum level of vitamin D, CRP and biochemical parameter in acute and chronic brucellosis treated with doxycycline-loaded solid lipid nanoparticles

Hosseini, Seyed Mostafa; Abbasalipourkabir, Roghayyeh; Jalilian, Farid Azizi; Asl, Sara Soleimani; Farmani, Abbas; Roshanaei, Ghodratollah; Arabestani, Mohammad Reza

doi:10.1016/j.genrep.2020.100940

Cited by 3 publications

(3 citation statements)

References 29 publications

(26 reference statements)

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“…Under the influence of Brucella, chronic body consumption and hypersplenism often accompany an abnormal haemogram (33,34). Creatinine is a measure of glomerular filtration function and the risk of chronicity increases when SCr increases.…”

Section: Discussionmentioning

confidence: 99%

A follow-up study of 100 patients with acute brucellosis for its prognosis and prevention

Bai,

Ta,

Zhao

et al. 2023

Front. Med.

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ObjectiveTo prevent chronic brucellosis, this study analysed the changes in patient antibody titers, and the trajectories of biochemical indicators at different stages of brucellosis, identified relevant biomarkers, and explored risk factors affecting the prognosis of brucellosis patients.MethodsA prospective cohort study was conducted to follow 100 patients with acute brucellosis. Laboratory serological test results [taken with a serum (tube) agglutination test (SAT)] and biochemical parameters (liver function, renal function, and hematological system) were measured repeatedly at four-time points: 0 weeks—baseline survey, 6 weeks after the first treatment, 12 weeks after the second treatment, and 3 months after the third treatment. The changes in the antibody titres and biochemical parameters at each time point were analysed for trend changes.ResultsOne hundred patients with acute brucellosis were enrolled in this follow-up study, with 100% retention in follow-up. By the third follow-up, 21 patients had turned subacute and 11 had turned chronic. One-way repeated measures analysis of variance results showed statistically significant differences (p < 0.01) across the time points for the following five indicators: alanine aminotransferase, aspartate aminotransferase, total bilirubin, serum creatinine (SCr) and platelet count. The clinical symptoms of patients in the acute stage were mainly joint pain, fatigue, and fever, while those in the chronic stage complained primarily of joint pain and fatigue. The results of multivariate logistic analysis showed that joint pain [odds ratio (OR) = 3.652, 95% confidence interval (CI) =1.379–9.672], monoarticular pain (OR = 6.356, 95% CI = 4.660–8.669), elevated SCr (OR = 15.804, 95% CI = 1.644–151.966) and elevated haemoglobin (Hb) (OR = 1.219, 95% CI = 1.065–1.736) were risk factors for poor prognosis (not cured or chronic) in patients with brucellosis.ConclusionThe trajectory of changes in patient SAT posirates and antibody titers can be used to distinguish patients with chronic brucellosis. The brucellosis is preventable and treatable, and the standard treatment can be effective in reducing the clinical symptoms of affected patients. If patients are not treated in a timely manner, joint pain, monoarticular pain, and elevated SCr are risk factors for patients who are not cured. Therefore, the treatment cycle for these patients should be extended.

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Section: Discussionmentioning

confidence: 99%

A follow-up study of 100 patients with acute brucellosis for its prognosis and prevention

Bai,

Ta,

Zhao

et al. 2023

Front. Med.

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“…CRP measurement provides information for the diagnosis and evaluation of infections, inflammatory disorders, and related diseases. To measure the vitamin D levels in different study groups, the Elisa kit (Arka Fanazma-Iran) was used according to the kit instructions 18 .…”

Section: Methodsmentioning

confidence: 99%

“…Vitamin D is another important trace element because it helps maintain calcium homeostasis, which is critical for both the innate and acquired immune systems 18 . According to studies, a healthy level of vitamin D in the host's serum increases the strength of the immune system against infections.…”

Section: Introductionmentioning

confidence: 99%

Co-delivery of streptomycin and hydroxychloroquine by labeled solid lipid nanoparticles to treat brucellosis: an animal study

Moez,

Hosseini,

kalhori

et al. 2023

Sci Rep

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Can brucellosis-related biochemical and immunological parameters be used as diagnostic and treatment indicators? The goal of this project was to look at biochemical parameters, trace elements, and inflammatory factors in the acute and chronic stages of brucellosis after treatment with streptomycin and hydroxychloroquine-loaded solid lipid nanoparticles (STR-HCQ-SLN). The double emulsion method was used for the synthesis of nanoparticles. Serum levels of trace elements, vitamin D, CRP, and biochemical parameters were measured in rats involved in brucellosis. The therapeutic effect of STR-HCQ-SLN was compared with that of free drugs. In both healthy and infected rats, serum concentrations of copper, zinc, iron, magnesium, potassium, and biochemical parameters of the liver were significantly different. By altering the serum levels of the aforementioned factors, treatment with STR-HCQ-SLN had a positive therapeutic effect on chronic brucellosis. Vitamin D levels declined (46.4%) and CRP levels rose (from 7.5 mg to less than 1 mg) throughout the acute and chronic stages of brucellosis. This study showed that by comparing the biochemical parameters and the levels of trace elements in the serum of healthy and diseased mice in the acute and chronic stages of brucellosis, it is possible to get help from other routine methods for diagnosis.

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Codelivery of Doxycycline and Hydroxychloroquine to Treatment of Brucellosis: An Animal Study

Hosseini

Farmany

Alikhani

et al. 2022

Journal of Nanomaterials

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Evaluation of various biochemical and immunological parameters in infectious diseases is one of the best indicators for a diagnosis and treatment process. The main goal of this project is to determine the effect of hydroxychloroquine and doxycycline loading into solid-lipid-nanoparticles (DOX-HCQ-SLN) on the both acute and chronic phases of brucellosis. In addition, evaluate some biochemical factors, trace elements, and inflammatory elements. Blood serum levels of Zn, Fe, Na, and K and hepatic biochemical parameters (AST, ALT, ALP, and TBil) were remarkably different between infected and healthy rats. Vitamin D was decreased, and CRP was increased in chronic and acute brucellosis. Quantitative evaluation of these mentioned parameters can be useful to diagnose brucellosis in advance. Due to the good effect of the synchronized use of hydroxychloroquine and doxycycline in the form of nanoparticles, the manipulation of these nanoparticles can help for better treatment and also reduction in brucellosis reinfection.

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Serum level of vitamin D, CRP and biochemical parameter in acute and chronic brucellosis treated with doxycycline-loaded solid lipid nanoparticles

Cited by 3 publications

References 29 publications

A follow-up study of 100 patients with acute brucellosis for its prognosis and prevention

A follow-up study of 100 patients with acute brucellosis for its prognosis and prevention

Co-delivery of streptomycin and hydroxychloroquine by labeled solid lipid nanoparticles to treat brucellosis: an animal study

Codelivery of Doxycycline and Hydroxychloroquine to Treatment of Brucellosis: An Animal Study

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