Kombucha tea ameliorates experimental autoimmune encephalomyelitis in mouse model of multiple sclerosis

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a mouse model for multiple sclerosis (MS), in which an inflammatory demyelination and axonal damage occurs. Kombucha tea is a fermented beverage made from kombucha mushroom, brewed tea, and sugar. In recent years kombucha tea has attracted interest due to its pharmacological properties like antioxidant effects. The aim of the present research was to test the therapeutic effect of kombucha tea in EAE. We induced EAE model in 18 female C57BL/6 mice by inoculation of myelin oligodendrocyte glycoprotein- ) in complete Freund's adjuvant emulsion. Then, in order to ameliorate EAE symptoms, we used kombucha tea. During the course of study clinical evaluation was assessed, and on the day 21 post-immunization, for evaluation of nitric oxide (NO), total antioxidants capacity and tumor necrosis factor-alpha (TNF-α), blood samples were taken from the heart of mice. The mice were sacrificed and brains and cerebellums of mice were removed for histological analysis. Our findings demonstrated that kombucha tea had beneficial effects on EAE by lower incidence, attenuation in the severity, and also a delay in the onset of disease. Histological analysis showed that inflammatory criteria including the number of infiltrated immune cells and plaques as well as demyelination in kombucha tea dosed mice were significantly lower than the control group. Also, in comparison with control mice, the serum levels of NO and TNF-α in kombucha teatreated mice were significantly decreased. Kombucha tea with its potential therapeutic effects and immunomodulatory properties might be proposed, after additional necessary tests and trials, for treatment of MS.

The protective effect of the Vacha rhizome extract on chronic stress-induced immunodeficiency in rat

“…Similarly Kombucha tea amelioratesd the autoimmune encephalomyelitis in mouse model of multiple sclerosis (Marzban et al. 2015 ). Few herbs viz.…”

Pre-treatment Effect of Kombucha Tea on Analgesia and Inflammation in Male Rat

“…These compounds help the nervous system in headaches and anxiety and prevent epileptic seizures (17). In animal models, it is demonstrated that the anti-inflammatory and antioxidant effects provided by the Kombucha tea can ameliorate the inflammation and oxidative stress in the pancreatic, hepatic, renal, and cardiac tissues of diabetic animals (18). An in vitro study showed that Kombucha tea reduced the ROS-induced apoptosis in murine hepatocytes probably due to its antioxidant activity (19).…”

Comparison of Phenolic Contents and Scavenging Activities of Miang Extracts Derived from Filamentous and Non-Filamentous Fungi-Based Fermentation Processes

“…Black teas, Oolong tea, and microbial fermented teas such as Kombucha and Pu-erh have also been reported to inhibit inflammation and related disease pathways, viz. atherosclerosis and cancer models via inhibition of NO production through iNOS, as well as inhibit other proinflammatory mediators such as interleukin-12, monocyte chemoattractant protein (MCP), and tumor necrosis factor-α (TNF-α) [31,40,85]. The result from this study corroborate positive impacts that have been reported for other traditional fermented teas such as Pu-erh, Oolong, Zijuan, and Kombucha, among others, considering the fermentation of both Miang and these teas' is mediated by microbes [6,86].…”