Komposisi Kimia, Kadar Albumin Dan Bioaktivitas Ekstrak Protein Ikan Gabus (Channa Striata) Alam Dan Hasil Budidaya

Chasanah, Ekowati; Nurilmala, Mala; Purnamasari, Ayu Ratih; Fithriani, Diini

doi:10.15578/jpbkp.v10i2.364

Cited by 44 publications

(49 citation statements)

References 14 publications

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“…For each fractions of aquadest and ethanol 50% extractions at 10 mg/mL, fraction 5-10 kDa had the highest activity, followed by fractions of >10 kDa, 3-5 kDa and finally <3 kDa. Chasanah et al (2015) reported that captopril with a concentration of 1 mg/ml had ACE inhibitory activity of 90.32%. The concentration of the snakehead fish fillet extract used for ACE inhibitory activity analysis in this research (10 mg/ml) was tenfold compared with the concentration of captopril (1 mg/ml) used by Chasanah et al (2015).…”

Section: Ace Inhibitory Activity In the Crude Extract And Its Fractionsmentioning

confidence: 99%

“…The properties of an antipyretic (Zakaria, Kumar, Mat Jais, Sulaiman, & Somchit, 2008), antiinflammatory (Zakaria et al, 2008) and antiosteoarthritic (Michelle, Shanti, & Loqman, 2004) were reported to be found in snakehead fish. Recently, Chasanah, Nurilmala, Purnamasari, and Fithriani, (2015) reported that the aquadest crude extract of snakehead fish fillet (50 mg/ml) was potential as an ACE inhibitor with activity equivalent to 1/10 of captopril, the commercial antihypertensi drug (5 mg/ ml). Those reported health benefits and nutrition showed that snakehead fish is a very potential source for discovering new bioactive compounds, particularly endogenous protein or peptide with ACE inhibitory activity.…”

Section: Introductionmentioning

confidence: 99%

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ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITORY ACTIVITY OF CRUDE AND FRACTIONATED SNAKEHEAD (Channa striata) MEAT EXTRACT

Budiari¹,

Chasanah

Suhartono³

et al. 2018

Squalen Bull. Marine Fisheries Postharvest Biotech.

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The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead meat is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous bioactive protein or peptide from snakehead meat and to fractionate the active compounds using ultrafiltration. The extraction in this research employs two kinds of solvents, e.i aquadest and 50% ethanol. Then, the extract is fractionated with the ultrafiltration using the 10,000; 5,000 and 3,000 MWCO membranes to separate the protein or peptide of with the sizes of >10 kDa, 5 – 10 kDa, 3 – 5 kDa and <3 kDa. The parameters being observed from the crude extracts and its fractions included the protein and peptide content, the ACE inhibitory activity (in vitro), and the protein and peptide profiles determined by using the SDS PAGE. The result of this research revealed that the snakehead meat contained ACE inhibitory endogenous bioactive protein or peptide. The 50% ethanol was more effective in extracting peptide sized <10 kDa than the aquadest. Yet, the aquadest was better in extracting higher molecular weight protein of >10 kDa than the 50% ethanol was. The bioactivity of 50% ethanol extraction was higher than the aquadest extraction. The highest bioactivity of 95.18% was gained from the fraction 5 – 10 kDa of 50% ethanol extract

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Section: Ace Inhibitory Activity In the Crude Extract And Its Fractionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITORY ACTIVITY OF CRUDE AND FRACTIONATED SNAKEHEAD (Channa striata) MEAT EXTRACT

Budiari¹,

Chasanah

Suhartono³

et al. 2018

Squalen Bull. Marine Fisheries Postharvest Biotech.

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“…Kandungan albumin ikan gabus dari sumber populasi berbeda memiliki kecenderungan berbeda pula. Kandungan albumin ikan gabus yang dikoleksi dari perairan umum lebih tinggi dibandingkan ikan gabus hasil budidaya (Chasanah et al, 2015). Dinyatakan pula bahwa ikan gabus asal Jawa Barat memiliki kandungan albumin lebih tinggi dibandingkan ikan gabus asal Jawa Timur dan Jawa Tengah (Susilowati et al, 2015).…”

Section: Pendahuluanunclassified

“…Sementara itu, sejumlah studi komparatif terhadap kandungan albumin ikan gabus telah dilakukan di Indonesia dan cenderung mengarah pada adanya perbedaan atau variasi kandungan albumin antar populasi. Dinyatakan oleh Susilowati et al (2015) dan Chasanah et al (2015) bahwa kandungan albumin C. striata dari Jawa Barat lebih tinggi (107,28 ± 3,19 mg/g) dibandingkan albumin C. striata dari Jawa Timur (91,10 24,08 mg / g) dan Jawa Tengah (75,79 ± 9,33 mg/g). Lebih lanjut, Fuadi et al (2017) melaporkan bahwa kandungan albumin ikan gabus asal Kabupaten Lamongan (daerah air payau) secara signifikan lebih tinggi (4,1 ± 0,96 mg / g) dibandingkan dengan kandungan albumin ikan gabus dari kabupaten Lumajang yang merupakan daerah air tawar (2,9 ± 0,87 mg/g).…”

Section: Sd Kk unclassified

KERAGAMAN DAN KORELASI KANDUNGAN ALBUMIN DENGAN KARAKTER PERTUMBUHAN PADA TIGA POPULASI IKAN GABUS (Chana striata)

Khasani

Astuti

2020

Jurnal Riset Akuakultur

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Albumin ikan gabus merupakan bahan farmasi bernilai tinggi, banyak dimanfaatkan untuk mengobati pasien pascaoperasi dan luka bakar. Penelitian ini bertujuan untuk menganalisis keragaman dan korelasi kandungan albumin terhadap karakter pertumbuhan pada tiga populasi ikan gabus (Chana striata) dari Sumatera (SM), Jawa (JW) dan Kalimantan (KL). Tahap awal dari penelitian ini adalah pemeliharaan larva-benih, karakterisasi dan analisis kandungan albumin ikan gabus dari tiga populasi. Kandungan albumin pada sampel daging (20 ekor ikan per populasi) dianalisis menggunakan reagen bromocresol green dan diukur dengan spektrofotometer pada 578 nm. Benih ikan gabus dari populasi SM, JW, dan KL dengan ukuran panjang total 28,9 ± 5,7 mm dan bobot badan 0,27 ± 0,09 g dari 12 famili, terdiri dari 4 famili untuk setiap populasi dipelihara secara acak dalam 12 buah kolam beton ukuran 25 m2 selama 75 hari. Kandungan albumin rata-rata ketiga populasi tidak berbeda nyata (P>0,05), yaitu 2,6 ± 0,4 g dL-1 (KL), 2,4 ± 0,3 g dL-1 (SM) dan 2,2 ± 0,4 g dL-1 (JV); dengan koefisien keragaman sebesar 12,60% (SM), 18,13% (JT) dan 17,0% (KL). Nilai korelasi antara panjang total, panjang standar, dan bobot badan dengan kandungan albumin tergolong rendah hingga sedang, yaitu secara berurutan sebesar -0,05; -0,03 dan -0,43 (SM), 0,42; 0,475 dan 0,34 (JW) dan -0,28; -0,35 dan -0,275 (KL). Hasil analisis tersebut menunjukkan bahwa keragaman kandungan albumin tidak berbeda antar populasi, sehingga pendekatan seleksi kurang efektif. Nilai korelasi antara karakter pertumbuhan terhadap kandungan albumin pada ikan gabus tergolong rendah. The snakehead fish (SHF) albumin is a highly valued pharmaceutical material widely used to treat postsurgery wounds and skin burns. This study’s purpose was to analyze the coefficient of variance and correlation of albumin trait to growth characters of three SHF populations: Sumatera (SM), Java (JV) and Kalimantan (KL). The first step of this study consisted of larval and seed rearing of three SHF populations followed up by characterization and analysis of their albumin content. The albumin contents of the SHF meat (20 fish for each population) were analyzed using bromocresol green reagent and measured using a spectrophotometer at 578 nm. The SHF seeds (28.9 ± 5.7 mm of total length and 0.27 ± 0.09 g of body weight) from 12 families consisted of four families for each population were reared in 12 concrete ponds measuring 25 m2 for 75 days. The averages of albumin content of the three populations were 2.6 ± 0.4 g dL-1 (KL) 2.4 ± 0.3 g dL-1 (SM) and 2.2 ± 0.4 g dL-1 (JV) and considered not significantly different (P> 0.05) with coefficients of variance of 12.60% (SM), 18.13% (JV) and 17.0% (KL). The correlation values of total length (TL), standard length (SL) and body weight (BW) with albumin content of the SHF were -0.05, -0.03, and -0.43 (SM); 0.42, 0.475, and 0.34 (JV); and -0.28, -0.35, dan -0.275 (KL) and classified as low to moderate. The results suggested that the variances of albumin content among the populations were not significantly different and the individual selection method was not effective.

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“…Hal ini sejalan dengan hasil penelitian yang dilakukan oleh Mukhlisul et al (2017) bahwa kadar protein tertinggi C. striata sebesar 0,389 mg/mL dan terendah sebesar 0,138 mg/ mL dari Kabupaten Lamongan, sedangkan Kabupaten Lumajang memiliki kadar protein tertinggi sebesar 0,155 mg/mL dan terendah sebesar 0,063 mg/mL. Chasanah et al (2015) menunjukkan bahwa kadar protein ikan gabus alam lebih tinggi dibandingkan dengan ikan gabus budidaya. Ikan gabus alam dengan bibit satu kilogram memiliki kadar protein sebesar 20,14%.…”

Section: Hasil Dan Pembahasanunclassified

Kadar Protein Terlarut dalam Albumin Ikan Gabus (Channa striata dan Channa micropeltes) Asal Bogor SOLUBLE ROTEIN CONCENTRATION IN SNAKEHEAD FISH ALBUMIN BOGOR ORIGIN (CHANNA STRIATA AND CHANNA MICROPELTES)

Alviodinasyari

Pribadi

Soejoedono

2019

JVet

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Snakehead fish is currently used by the public as a health treatment because it has a higher content of albumin protein than others. The Research aimed was determined the protein quality and comparison levels in two species of snakehead fish, Channa striata and Channa micropeltes, derived from Bogor Regency. Protein concentration level of C. micropeltes and C. striata after drying for five minutes as much as 0.830 mg/mL and 0.803 mg/mL and these concentration were higher than dried for 10 and 15 minutes. Protein concentration levels of the two species were not significantly different. The molecular weight of albumin protein from both species were 65.56 kDa to dried for five minutes, 65.08 kDa to dried for 10 minutes, and 64.91 kDa to dried for 15 minutes. The Results concluded that there were no differences in protein levels in the fish meat and skin of the two species. Drying treatment affects the molecular weight of snakehead fish albumin protein.

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Komposisi Kimia, Kadar Albumin Dan Bioaktivitas Ekstrak Protein Ikan Gabus (Channa Striata) Alam Dan Hasil Budidaya

Cited by 44 publications

References 14 publications

ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITORY ACTIVITY OF CRUDE AND FRACTIONATED SNAKEHEAD (Channa striata) MEAT EXTRACT

ANGIOTENSIN CONVERTING ENZYME (ACE) INHIBITORY ACTIVITY OF CRUDE AND FRACTIONATED SNAKEHEAD (Channa striata) MEAT EXTRACT

KERAGAMAN DAN KORELASI KANDUNGAN ALBUMIN DENGAN KARAKTER PERTUMBUHAN PADA TIGA POPULASI IKAN GABUS (Chana striata)

Kadar Protein Terlarut dalam Albumin Ikan Gabus (Channa striata dan Channa micropeltes) Asal Bogor SOLUBLE ROTEIN CONCENTRATION IN SNAKEHEAD FISH ALBUMIN BOGOR ORIGIN (CHANNA STRIATA AND CHANNA MICROPELTES)

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